Fig 1: Interference of ITGB3 expression decreased the phosphorylation of FAK-MAPK pathway in Jurkat cells. (a) The total protein expression level and phosphorylation level of FAK, cRAF, MEK and ERK1/2 in Jurkat (ctrl), sh-NC and sh-ITGB3 cells detected by immunoblot. The expression of actin was applied as internal control. (b) The column graph of relative expression level of phosphorylation of FAK, cRAF, MEK and ERK1/2 in Jurkat (ctrl), sh-NC and sh-ITGB3 cells detected by immunoblot. The relative expression levels were calculated by the ratio of density of phosphorylation band and total protein band. (c) The total protein expression level and phosphorylation level of FAK, cRAF, MEK and ERK1/2 in control (ctrl) and Jurkat cells treated with ITGB3 antibodies (anti-ITGB3). The expression level was detected by immunoblot. The expression of actin was applied as internal control. (d) The column graph of relative expression level of phosphorylation of FAK, cRAF, MEK and ERK1/2 in control (ctrl) and Jurkat cells treated with integrinavß3 antibodies (anti-ITGB3) detected by immunoblot. The relative expression levels were calculated by the ratio of density of phosphorylation band and total protein band. ***P<.05, ***P<.01, **P<.001.
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