IL-6 Antibody AF-206-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-6 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-206-NA
NCBI Gene AliasesCDF, HSF, BSF-2, IL-6, IFNB2, HGF, BSF2, IFN-beta-2
ApplicationsAffinity Assay,Bioassay,ELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Proximity Ligation Assay,Neutralization,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Functional Assay,ELISA Capture,ELISA Detection
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetIL-6
HostGoat
ReactivityHuman,Mouse,Primate - Macaca mulatta (Rhesus Macaque)
IsotypeIgG
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
NCBI Full Gene Nameinterleukin 6
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human IL-6, Pro29-Met212, Accession # P05231
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Mitochondrial Transplantation Suppresses mtDNA-cGAS/STING-Mediated Innate Immunity by Enhancing PINK1/Parkin-Dependent Mitophagy to Attenuate Keloid FibrosisAntioxidants (Basel)September 4, 2026Wenjing Wang, Yuanbo Liu, Jipeng Song, Zouzou Yu, Zixiang Chen, Hu Jiaoevaluate the contribution of fibroblast-derived cytokines, neutralizing antibodies against human IL-6 (AF-206-NA; R&D Systems, Minneapolis, MN, USA) and CCL-2 (AF-279-NA; R&D Systems) were added to the co-culture

(2) Interleukin 4-driven loss of stromal LIF signaling affects immune responses and cell-cell adhesion in atopic dermatitisiScienceAugust 21, 2026Yarden Feller, Kiril Malovitski, Sari Assaf, Yan Stein, Sivan Friedman, Dafna Tussia-Cohen, Lubna Khair, Rawaa Ishtewy, Tzachi Hagai, Avraham Unterman, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-LIF,"Abcam, UK",Cat# ab138002; RRID: AB_3083551 Anti-IL-6,"R&D Systems, USA",Cat# AF-206-NA; RRID: AB_354392 Anti-DSG1,"Santa Cruz Biotechnology, USA",Cat# sc-137164; RRID: AB_2093310 Anti-α-Tubulin,"Abcam,

(3) Monocytes and neutrophils promote cardiac fibroblast pro-fibrotic phenotypes through IL-6 and MIFFront Cell Dev BiolMay 12, 2026Zhongxiao Cong, Aleksandar Ivetic, Maddy Parsonsμg/mL, Novus 1A11-4G0) and anti-HSC70 (0.2 μg/mL, Santa-Cruz Biotech SC-7298). Anti-IL6 (Bio-techne, AF-206-NA) and anti-MIF (Merck, MABF111) neutralizing antibodies were used at 500 ng/mL. 2.1.1 Cell culture Primary

(4) Loss of sirtuin 3 disrupts cellular senescence signaling pathwaysGeroScienceMay 6, 2026Niharika Kura, Bronwyn A. Mogck, Samantha T. Jezak, Michael C. Velarde, Christopher D. Wileytriton-X-100 for 30 min. Cells were washed again and incubated overnight in IL6 antibody (R&D Systems AF-206-NA), washed, and incubated in donkey-anti-Goat secondary antibody conjugated to ALEXA-488. Cells were counterstained

(5) Macrophage-derived IL-6 reprograms lipid metabolism to promote colorectal cancer development through USP14-mediated FASN deubiquitinationJournal of Translational MedicineFebruary 26, 2026Liu Yunxin, Xian Xirui, Zhu Xinyi, Wang Chenjing, Zhang Ting, Fang Kanghui, Yu Haixia, Chang Zude, Huang Wan, Zhu Yubing, et al.C0105S, Beyotime). For immunohistochemistry, the slices were incubated with antibodies against IL-6 (Cat#AF-206-NA, R&D Systems, Minneapolis, Minnesota, USA), p-STAT3 (Cell Signaling Technology, Danvers, Massachusetts,

(6) EGFR-mutant transformed small cell lung cancer harbors intratumoral heterogeneity targetable with MEK inhibitor combination therapyJCI insightJanuary 23, 2026Ogino A, Vajdi A, Mu XJ, Mahadevan NR, Ngo K, Booker MA, Cejas P, Okoro JJ, Xu M, Springer BF, et al.Mycoplasma Plus PCR Primer set (Agilent). LIF neutralizing antibody (AF-250-NA), IL-6 neutralizing antibody (AF-206-NA), and control antibody (AB-108-C) were purchased from R&D Systems. Human EGF recombinant protein was

(7) FGF4 drives tumor progression in triple-negative breast cancer via IL6/STAT3-mediated macrophage M2 polarization and immune suppressionCell divisionOctober 2, 2025Zhang X, Zhang S, Han Y.signaling pathway in FGF4-mediated macrophage polarization, the IL6-neutralizing antibody (1 µg/mL, AF-206-NA, R&D Systems, USA) or the STAT3 activator Colivelin (10 µM, 3945, Tocris Bioscience, UK) was added to

(8) Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte functionNature microbiologyDecember 1, 2024Sinclair JE, Vedelago C, Ryan FJ, Carney M, Redd MA, Lynn MA, Grubor-Bauk B, Cao Y, Henders AK, Chew KY, et al.

(9) IL-33-dependent NF-κB activation inhibits apoptosis and drives chemoresistance in acute myeloid leukemiaCytokineAugust 1, 2024Yan M, Chen X, Ye Q, Li H, Zhang L, Wang Y.

(10) Unravelling heterogeneous effects of cancer‑associated fibroblasts on poor prognosis markers in breast cancer EM‑G3 cell line: In vitro‑targeted treatment (anti‑IL-6, anti‑VEGF-A, anti‑MFGE8) based on transcriptomic profilingOncology reportsJanuary 1, 2024Urban L, Novák Š, Čoma M, Dvořánková B, Lacina L, Šáchová J, Hradilová M, Svatoňová P, Kolář M, Strnad H, et al.

(11) Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia PathogenesisInternational journal of molecular sciencesDecember 21, 2023Ozmen A, Nwabuobi C, Tang Z, Guo X, Larsen K, Guller S, Blas J, Moore M, Kayisli UA, Lockwood CJ, et al.

(12) Suppression of TGFβ-Induced Interleukin-6 Secretion by Sinulariolide from Soft Corals through Attenuation of the p38-NF-kB Pathway in Carcinoma CellsInternational journal of molecular sciencesJuly 19, 2023Yang JL, Lin WL, Tai SB, Ciou YS, Chung CL, Chen JJ, Liu PF, Lin MW, Chen CL.

(13) Extending the dynamic range of biomarker quantification through molecular equalizationNature communicationsJuly 13, 2023Newman SS, Wilson BD, Mamerow D, Wollant BC, Nyein H, Rosenberg-Hasson Y, Maecker HT, Eisenstein M, Soh HT.

(14) Effects of interleukin 1β on long noncoding RNA and mRNA expression profiles of human synovial fluid derived mesenchymal stem cellsScientific reportsMay 19, 2022Sun YP, Lu YY, Chen J, Bao JH, Zhang H, Sun JD, Liao WT.

(15) Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal CellsCancersMarch 9, 2022Pereira JFS, Bessa C, Matos P, Jordan P.

(16) Inhibition of relaxin autocrine signaling confers therapeutic vulnerability in ovarian cancerThe Journal of clinical investigationApril 1, 2021Burston HE, Kent OA, Communal L, Udaskin ML, Sun RX, Brown KR, Jung E, Francis KE, La Rose J, Lowitz J, et al.ascites for 72 hours in the absence or presence of αIL-6 neutralizing antibody (10 μg/mL, R&D Systems AF-206-NA). For STAT3 and NFKB inhibition, cells were treated with Stattic (1 μM, Selleckchem S7024) or QNZ (5nM,

(17) Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide AnalysisMolecular & cellular proteomics : MCPJanuary 1, 2021Wik L, Nordberg N, Broberg J, Björkesten J, Assarsson E, Henriksson S, Grundberg I, Pettersson E, Westerberg C, Liljeroth E, et al.conjugation reaction. Oligonucleotide performance assessment experiments were run using IL6 (MAB206 and AF-206-NA, R&D Systems) and HE4 (Agrisera) as a test system to determine the optimal design of the assay-specific

(18) Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formationNature communicationsMarch 5, 2020Ji Q, Zhou L, Sui H, Yang L, Wu X, Song Q, Jia R, Li R, Sun J, Wang Z, et al.

(19) A homogeneous bioluminescent immunoassay to probe cellular signaling pathway regulationCommunications biologyJanuary 1, 2020Hwang BB, Engel L, Goueli SA, Zegzouti H.purchased from LC Laboratories. FM-381 (#S8541) was purchased from Selleckchem. Anti-human IL-6 antibody (#AF-206-NA) was purchased from R&D Systems and was used in a titration from 0.76 ng/ml–20 µg/ml. ACTEMRA® (tocilizumab,

(20) Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to agingJCI insightJune 11, 2019Muñoz DP, Yannone SM, Daemen A, Sun Y, Vakar-Lopez F, Kawahara M, Freund AM, Rodier F, Wu JD, Desprez PY, et al.MS-889-P1 and MS-218-P1), ATM (Abcam Y-170), phospho-ATM (Upstate, 05-740), IL-6 (R&D Systems, MAB2061 and AF-206-NA), IL-8 (R&D Systems, MAB208), MICA/B (R&D Systems, MAB13001; Santa Cruz Biotechnology Inc., sc-5460),

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IL-6 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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IL-6 Antibody from R&D Systems, a Bio-Techne Brand

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