VEGF 165 Antibody AB-293-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemVEGF 165 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAB-293-NA
ApplicationsBioassay,Complex Application,ELISA Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization
TargetVEGF
ReactivityHuman,Mouse,Primate - Macaca mulatta (Rhesus Macaque),Rabbit,Rat,Xenograft
IsotypeIgG
HostGoat
Quantity1 mg
Conjugate/TagUnconjugated
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165, Ala27-Arg191, Accession # AAV38412
ConcentrationLYOPH
PurityProtein A or G purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namevascular endothelial growth factor A
NCBI Gene AliasesMVCD1, VEGF, VPF

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human VEGF165 and human VEGF121 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse VEGF and recombinant rat VEGF is observed.

Sizes Available: 1 mg

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) VEGF-A165 is the predominant VEGF-A isoform in platelets, while VEGF-A121 is abundant in serum and plasma from healthy individualsPloS oneJanuary 1, 2023Yamakuchi M, Okawa M, Takenouchi K, Bibek A, Yamada S, Inoue K, Higurashi K, Tabaru A, Tanoue K, Oyama Y, et al.

(2) Attenuating Adaptive VEGF-A and IL8 Signaling Restores Durable Tumor Control in AR Antagonist-Treated Prostate CancersMolecular cancer research : MCRJune 3, 2022Maxwell PJ, McKechnie M, Armstrong CW, Manley JM, Ong CW, Worthington J, Mills IG, Longley DB, Quigley JP, Zoubeidi A, et al.described (11). Human IL8 monoclonal antibody (RRID:AB_2249110; #MAB208), human VEGF mAb (RRID:AB_354341; #AB293NA) and isotype-matched IgG (RRID:AB_357344; #MAB002) were obtained from R&D Systems. MDV3100 (#S1250)

(3) Retinopathy of prematurity shows alterations in Vegfa(164) isoform expressionPediatric researchJune 1, 2022Mezu-Ndubuisi OJ, Song YS, Macke E, Johnson H, Nwaba G, Ikeda A, Sheibani N.incubated overnight with the primary antibodies against the molecule of interest. Vegf (R&D Systems, AB-293-NA) and protein kinase C-alpha (PKCalpha, Sigma, P4334) staining were done to detect total Vegf expression

(4) High methylation of lysine acetyltransferase 6B is associated with the Cobb angle in patients with congenital scoliosisJournal of translational medicineMay 24, 2020Wu Y, Zhang H, Tang M, Guo C, Deng A, Li J, Wang Y, Xiao L, Yang G.anti-c-Myc antibody, 1:1000 dilution, cat no. ab32072, Abcam; anti-VEGF antibody, 1:1000 dilution, cat no. AB-293-NA, Bio-Techne; anti-GAPDH antibody, 1:3000 dilution, cat no. sc-47724, Santa Cruz Biotechnology. After

(5) Vasculogenic mimicry formation in EBV-associated epithelial malignanciesNature communicationsNovember 27, 2018Xiang T, Lin YX, Ma W, Zhang HJ, Chen KM, He GP, Zhang X, Xu M, Feng QS, Chen MY, et al.Protein-tech, 1:5000 for WB), β-Tubulin (2128, Cell Signaling Technology, 1:2000 for WB), and VEGF (AB-293-NA, R&D Systems. 1 μg mL−1 for neutralization). Horseradish peroxidase-conjugated goat anti-mouse/rabbit

(6) Platelet-rich plasma decreases fibroblastic activity and woven bone formation with no significant immunohistochemical effect on long-bone healing: An experimental animal study with radiological outcomesJournal of orthopaedic surgery (Hong Kong)May 1, 2018Canbeyli İD, Akgun RC, Sahin O, Terzi A, Tuncay İC.antihuman Pro-BMP-2 antibody, (MAB2260, R & D Systems, Inc., Minneapolis, Minnesota, USA) and VEGF (AB-293-NA, R & D Systems, Inc.) antibody staining were measured.10 They were graded immunohistochemically as none

(7) High Plasma Lipid Levels Reduce Efficacy of Adenovirus-Mediated Gene TherapyScientific reportsMarch 24, 2017Kivelä AM, Huusko J, Gurzeler E, Laine A, Dijkstra MH, Dragneva G, Andersen CB, Moestrup SK, Ylä-Herttuala S.sections fixed with 4% paraformaldehyde in 7.5% sucrose for 4 h. hVEGF-A protein was immunostained with AB-293-NA (1:500, R&D Systems), endothelium with lectin antibody (Biotonylated Griffonia (Bandeiraea) Simplicifolia

(8) The truncated somatostatin receptor sst5TMD4 stimulates the angiogenic process and is associated to lymphatic metastasis and disease-free survival in breast cancer patientsOncotargetSeptember 13, 2016Gahete MD, Rincón-Fernández D, Durán-Prado M, Hergueta-Redondo M, Ibáñez-Costa A, Rojo-Sebastián A, Gracia-Navarro F, Culler MD, Casanovas O, Moreno-Bueno G, et al.against human sst5TMD4 previously described [6, 10] or the specific goat anti-human VEGF antibody (AB-293-NA; R&D systems, Minneapolis, MN, USA) and the appropriate secondary antibodies (anti-rabbit or anti-goat

(9) Epithelial-mesenchymal transition, proliferation, and angiogenesis in locally advanced cervical cancer treated with chemoradiotherapyCancer medicineAugust 1, 2016Rojas-Puentes L, Cardona AF, Carranza H, Vargas C, Jaramillo LF, Zea D, Cetina L, Wills B, Ruiz-Garcia E, Arrieta O.saline (PBS) to reduce unspecific staining, and incubated for 60 min with polyclonal anti‐human VEGF (AB‐293‐NA, R&D Systems AG, Oxford, UK) diluted 1:100 in PBS. After three washes with PBS, samples were incubated

(10) Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies-Synthesis and CharacterizationNanomaterials (Basel, Switzerland)August 7, 2015Tan G, Kantner K, Zhang Q, Soliman MG, Del Pino P, Parak WJ, Onur MA, Valdeperez D, Rejman J, Pelaz B.case, antibodies against HRP (anti-peroxidase, Sigma Aldrich) or against VEGF (anti-VEGF, R&D systems, AB-293-NA) where they are linked to the NPs. As described above, the antibodies were optionally tagged with FITC.

(11) A phase Ib/II translational study of sunitinib with neoadjuvant radiotherapy in soft-tissue sarcomaBritish journal of cancerDecember 9, 2014Lewin J, Khamly KK, Young RJ, Mitchell C, Hicks RJ, Toner GC, Ngan SY, Chander S, Powell GJ, Herschtal A, et al.Immunohistochemistry for VEGF-A was with polyclonal antibodies to human VEGF165 (R&D Systems catalogue number AB-293-NA) according to the manufacturer’s instructions; for VEGF-C was with affinity-purified polyclonal antibody

(12) Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylationBiotechnology lettersSeptember 1, 2014Litvinov J, Hagström AE, Lopez Y, Adhikari M, Kourentzi K, Strych U, Monzon FA, Foster W, Cagle PT, Willson RC.mixed with 240 μl polyclonal anti-human Vascular Endothelial Growth Factor (VEGF) antibody (1 mg/ml, AB-293-NA, R&D Systems) and 240 μl 3 M (NH4)2SO4. After 24 h incubation at 37 °C with slow tilt rotation, the

(13) Consumption of a Western-style diet during pregnancy impairs offspring islet vascularization in a Japanese macaque modelAmerican journal of physiology. Endocrinology and metabolismJuly 1, 2014Pound LD, Comstock SM, Grove KL.

(14) Extensive podocyte loss triggers a rapid parietal epithelial cell responseJournal of the American Society of Nephrology : JASNMay 1, 2014Hakroush S, Cebulla A, Schaldecker T, Behr D, Mundel P, Weins A.

(15) Lysophosphatidic acid protects human mesenchymal stromal cells from differentiation-dependent vulnerability to apoptosisTissue engineering. Part AApril 1, 2014Binder BY, Genetos DC, Leach JK.GM were exposed to SD/H with 25 mM LPA and the saturating addition of 10 mg/mL VEGF165/121 antibody (AB293-NA; R&D Systems, Minneapolis, MN)4 or 5 ng/mL recombinant VEGF (Lonza) with 25 mM LPA and 10 mM Ki16425

(16) Differential expression of vascular endothelial growth factor in high- and low-metastasis cell lines of salivary gland adenoid cystic carcinomaAnticancer researchFebruary 1, 2014Kondo S, Mukudai Y, Soga D, Nishida T, Takigawa M, Shirota T.

(17) Bioceramic-mediated trophic factor secretion by mesenchymal stem cells enhances in vitro endothelial cell persistence and in vivo angiogenesisTissue engineering. Part AJuly 1, 2012He J, Decaris ML, Leach JK.survival and persistence by inhibiting the effect of VEGF secretion by MSCs with a human VEGF antibody (AB-293-NA, R&D Systems, Minneapolis, MN). Per manufacturer’s instructions, 3–6 mg/mL of antibody is necessary

(18) Differentiation-dependent secretion of proangiogenic factors by mesenchymal stem cellsPloS oneJanuary 1, 2012Hoch AI, Binder BY, Genetos DC, Leach JK.conditioned media was neutralized by the saturating addition of 10 µg/mL anti-human VEGF16512/antibody (AB-293-NA, R&D Systems) [31] diluted in PBS. Control groups were administered an equivalent volume of PBS. ECFC

(19) Exogenous recombinant dimeric neuropilin-1 is sufficient to drive angiogenesisThe Journal of biological chemistryJanuary 7, 2011Uniewicz KA, Cross MJ, Fernig DG.

(20) Mimicking nature by codelivery of stimulant and inhibitor to create temporally stable and spatially restricted angiogenic zonesProceedings of the National Academy of Sciences of the United States of AmericaOctober 19, 2010Yuen WW, Du NR, Chan CH, Silva EA, Mooney DJ.layer containing 4 μg of VEGF and two surrounding 1-mm layers each incorporating 20 μg of anti-VEGF (R&D Systems AB-293-NA) (AVA for anti-VEGF-VEGFanti-VEGF). Quantification of Protein Release Kinetics. The release kinetics

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Human VEGF165 Antibody

VEGF 165 Antibody

VEGF 165 Antibody

VEGF 165 Antibody

VEGF 165 Antibody

VEGF 165 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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VEGF 165 Antibody from R&D Systems, a Bio-Techne Brand

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