Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb 2478S from Cell Signaling Technology

Product Specs
ItemPhospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number2478S
Quantity100 µl
ApplicationsW
ReactivityH, M
TargetVEGFR2 (Tyr1175) phosphate
ModificationsVEGFR2 (Tyr1175) phosphate
HostRabbit
NCBI Gene AliasesCD309, FLK1, VEGFR, VEGFR2
ClonalityMonoclonal
NCBI Full Gene Namekinase insert domain receptor
Research AreaRTK
IsotypeIgG
Clone19A10
Molecular Weight230

Questions and Answers

Can this antibody be used to study the effects of various compounds on VEGFR-2 phosphorylation in endothelial cells?

Yes, the Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb has been utilized in studies investigating the effects of compounds such as salinomycin and proanthocyanidins on VEGFR-2 phosphorylation in human umbilical vein endothelial cells (HUVECs). These studies have shown that these compounds can inhibit VEGFR-2 phosphorylation, thereby affecting angiogenesis and endothelial cell behavior.

Is this antibody effective for detecting VEGFR-2 phosphorylation in mouse models?

The antibody has been shown to effectively detect phosphorylated VEGFR-2 in various studies, including those involving mouse models. For instance, it was used in research examining the role of VEGFR-2 in ischemic stroke, where increased expression of VEGFR-2 was noted in knockout mice, indicating its relevance in vivo.

What applications has this antibody been cited in, particularly regarding cancer research?

The Phospho-VEGF Receptor 2 (Tyr1175) antibody has been cited in numerous studies focusing on cancer, particularly in understanding the mechanisms of angiogenesis in tumors. For example, it has been used to assess the impact of different treatments on VEGFR-2 signaling pathways in various cancer types, including glioma and breast cancer.

Does this antibody cross-react with other VEGF receptors, and how might that affect experimental outcomes?

Yes, this antibody may cross-react with VEGFR1, which could potentially influence experimental outcomes. Researchers should consider this cross-reactivity when interpreting results, especially in experiments designed to specifically target VEGFR-2 signaling pathways.

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Kuanxiong aerosol protects against coronary microvascular dysfunction through improving angiogenesis via HIF-1α-ITGAX axisFront PharmacolAugust 13, 2026Yuanqing Jiang, Kai Qian, Beibo Li, Mengmeng Xu, Pengfei OuYang, Yangyang Zhu, Jiaxu Wu, Xiaoying Shi, Yi Luo, Yao Xie, et al.Normal rabbit IgG,CST,2729,IP: 1:100 Hydroxy-HIF-1α (pro564),CST,3434,WB: 1:1000 FAK,CST,3285,WB: 1:1000 p-VEGFR2,CST,2478,WB: 1:1000 VEGFR2,CST,2479,WB: 1:1000 ITGAX,CST,45581,WB: 1:1000 HIF-1α,CST,36169,WB: 1:1000IP: 1: 100

(2) Carboxymethyl Chitosan Promotes Osteogenesis Via p38/VEGFR2 and Shows Translational Potential in Sinus Floor ElevationInt Dent JAugust 8, 2026Xinda Li, Zhijia Liu, Xinzhu Liu, Jianing Wang, Jinlai Bao, Wuwei LiChina, catalogue no. 26415-1-AP), p-VEGFR2 (Tyr1175) (Cell Signalling Technology, USA, catalogue no. 2478), RUNX2 (Proteintech, China, catalogue no. 20700-1-AP), ALP/ALPL (Proteintech, China, catalogue no.

(3) VEGFR2 dimerization kinetics on the cell surface determined by microscopy and mathematical modelingBiophysical JournalJuly 21, 2026Jaime Guerrero, Zachariah Malik, Fnu Bilal, Soma Jana, Aparajita Dasgupta, Khuloud Jaqamanwas replaced with 1:100 anti-phospho-VEGFR2 antibody (19A10; Cell Signaling Technology, Danvers, MA, 2478) diluted in blocking buffer to detect phosphorylated VEGFR2. Cells were then washed three times for

(4) Endothelial VEGFR-2 Activation Precedes Severe Mucosal Injury in TNBS-Induced ColitisInternational Journal of Molecular SciencesJune 27, 2026Sabrina Ceccariglia, Diego Sibilia, Alice Scattolini, Valentina Saccone, Ornella Parolini, Alessandro Armuzzi, Alfredo Papa, Antonio Gasbarrini, Fabrizio PizzolanteUSA, Cat. #2479; 1:1000) and p-VEGFR-2 (Tyr1175) (Cell Signaling Technology, Danvers, MA, USA, Cat. #2478; 1:1000). Membranes then were incubated with secondary HRP-conjugated antibody for 1 h at room temperature. Signals

(5) Inhibition of miR-181c-5p Rescues Diabetes-Impaired Angiogenesis in Ischemia and Wound HealingDiabetesJune 1, 2026Emma L. Solly, Yingjun Luo, Khalia R. Primer, Jocelyne Mulangala, Belinda A. Di Bartolo, Stephen J. Nicholls, Peter J. Psaltis, Zhen Bouman Chen, Christina A. Bursill, Joanne T.M. TanCell Signaling Technology), VEGFA (1:1,000; ab46154, Abcam), phosphorylated (Tyr1175) VEGFR2 (1:1,000; 2478, Cell Signaling Technology), total VEGFR2 (1:1,000; 2479, Cell Signaling Technology), phosphorylated

(6) Epac1 deletion attenuates Müller glial pathological activation and mitigates retinal neurodegeneration in ischemia-induced retinopathyJournal of Advanced ResearchJune 1, 2026Shuizhen Shi, Fan Xia, Zhongqiao Lu, Erick Palacios, Fang Mei, Massoud Motamedi, Xiaodong Cheng, Hua Liu, Wenbo ZhangCell Signaling Technology, Beverly, MA), VEGFR2 (2479, Cell Signaling Technology), pVEGFR2 (Y1175) (2478, Cell Signaling Technology), ERK1/2 (9102, Cell Signaling Technology), pERK1/2 (4377, Cell Signaling Technology

(7) HK2 promotes angiogenesis through H3K18 lactylation in peripheral arterial diseaseCommunications BiologyMay 28, 2026Jing Chen, Yue Wang, Xuan Wang, Jin Li, Yu Liu, Jipeng Zhou, Chuanchang Li, Wanzhou Wu, Yongping Bai(#19987-1-AP; Proteintech), VEGFA (#19003-1-AP; Proteintech), VEGFR2 (#A5609; ABclonal), p-VEGFR2 (#2478S; Cell Signaling Technology), FAK (#A11131; ABclonal), p-FAK(8556S; Cell Signaling Technology), PI3K(#4257S;

(8) Tumor-like proliferation of CCM3 knockout endothelial cells: insights from semaxinib treatment and transcriptome profiling of co-culturesActa Neuropathol CommunApril 20, 2026Valeriia V. Saenko, Janne L. Feldmann, Dariush Skowronek, Debora Singer, Ole J. Schamuhn, Doreen Biedenweg, Sander Bekeschus, Ute Felbor, Matthias Rath, Robin A. Pilzantibodies against VEGFR2 (#2479S, 1:200, 55B11, Cell Signaling Technology) or p-VEGFR2 (Tyr1175) (#2478S, 1:100, Cell Signaling Technology). After overnight incubation at 4 °C, samples were washed and incubated

(9) FRZB-induced anti-angiogenic effect via Caveolin-1-mediated TGFβ signallingNature CommunicationsApril 7, 2026Ching-Jou Chen, Lei Zhou, Han-Tang Chen, Veluchamy Amutha Barathi, Beiying Qiu, Asfa Alli-Shaik, Rui Ning Chia, Seok Ting Lim, Chaemin Joo, Ning Cheung, et al.(rabbit monoclonal, CST, Cat# 2479S, Lot# 18), Phospho-VEGF Receptor 2 (rabbit monoclonal, CST, Cat# 2478S, Lot# 16), SMAD3 (rabbit monoclonal, CST, Cat# 9523S, Lot# 5), Phospho-SMAD3 (rabbit monoclonal, CST,

(10) Vascular Endothelial Growth Factor-D Improves Lung Vascular Integrity During Acute Lung InjuryCirc ResMarch 13, 2026Yongdae Yoon, Lokesh Sharma, Wenwen Tang, Shannon Kirk, Micha Sam Brickman Raredon, Farida Ahangari, Johad Khoury, Hong Qian, Yunbo Ke, Mohan Tulapurkar, et al.Light Chain 2 (#3674), VEGFR2 (#2479), Tyr951 Phospho-VEGFR2 (#2471), and Tyr1175 Phospho-VEGFR2 (#2478) from Cell Signaling Technology (Danvers); ICAM-1 (Santa Cruz Biotechnologies, # sc-8439); Beta-actin

(11) Vascular Endothelial Growth Factor-D Improves Lung Vascular Integrity During Acute Lung InjuryCirc ResMarch 13, 2026Yongdae Yoon, Lokesh Sharma, Wenwen Tang, Shannon Kirk, Micha Sam Brickman Raredon, Farida Ahangari, Johad Khoury, Qian Hong, Yunbo Ke, Mohan Tulapurkar, et al.Light Chain 2 (#3674), VEGFR2 (#2479), Tyr951 Phospho-VEGFR2 (#2471), and Tyr1175 Phospho-VEGFR2 (#2478) from Cell Signaling Technology (Danvers); ICAM-1 (Santa Cruz Biotechnologies, # sc-8439); Beta-actin

(12) SHP2 regulates VEGFR2 Y1175/PLCγ signaling to impair tumor endothelial barrier stabilityiScienceFebruary 20, 2026Polina Kremmyda, Sara Owad, Sagnik Pal, Elvira Wildheim, Catarina Chanoca, Cecilia Lindskog, Elin Sjöberganti-VE-Cadherin pY731,In-house,Jin et al.29 Rabbit anti-phospho-VEGFR2 (Tyr1175),Cell Signaling Technology,2478S; RRID: AB_331377 Rabbit anti-VEGFR2,Cell Signaling Technology,2479S; RRID: AB_2212507 Mouse anti-Yes,BD Biosciences

(13) Cytoplasmic LIM domain only 2 enhances tumor endothelial cell migration through integrin β1-mediated focal adhesion signalingActa Neuropathologica CommunicationsFebruary 4, 2026Min Ji Park, Junseok Jang, Cheol Gyu Park, Minseo Gwak, Jong-Whi Park, Hyunggee KimSanta Cruz, USA), anti-ITGB1 (1:2 000) (A19072; ABclonal, South Korea), anti-p-VEGFR2 (Y1175; 1:1 000) (2478S; Cell Signaling, Danvers, MA, USA), anti-VEGFR2 (1:500) (2479S; Cell Signaling Technology, USA), anti-HIF1α

(14) GlycoRNA complexed with heparan sulfate regulates VEGF-A signallingNatureJanuary 28, 2026Peiyuan Chai, Sina Kheiri, Andrew Kuo, Jessica Shah, Lauren Kageler, Ruiqi Ge, Jonathan Perr, Jennifer Porat, Charlotta G. Lebedenko, Joao M. L. Dias, et al.4696S, Cell Signaling Technology; immunoblot 1:500), rabbit monoclonal anti-phospho-VEGFR2(Tyr1175) (2478S, Cell Signaling Technology; immunoblot 1:500), rabbit polyclonal anti-VEGFR2 (26415-AP, Proteintech;

(15) Aberrant VEGFR2 supports tumor growth by extracellular matrix remodelingCell Death & DiseaseJanuary 15, 2026Michela Corsini, Cosetta Ravelli, Mattia Domenichini, Anna Ventura, Camilla Maggi, Elisa Moreschi, Mirko Tamma, Chiara Romani, Claudia Piccoli, Elisabetta Grillo, et al.antibody (pThr202/Thr204, #9101- Cell Signaling Technology, Danvers, MA, USA) antibody or pVEGFR2 (#2478.-Cell Signaling Technology) when indicated. Antigen retrieval was performed in Citrate Buffer solution

(16) Prickle4 Drives Microenvironmental Remodeling and Resistance to Parp Inhibition in IDH‐Mutant GliomaAdvanced ScienceJanuary 1, 2026Ju Yang, Hua Yang, Yifan Yuan, Chenyang Zhang, Ziwei Fu, Yanyan Chen, Yinghong Xiong, Shuyu Chen, Kexin Ling, Ying Liu, et al.1:1000); Vinculin (Sigma‐Aldrich, V4505, 1:5000); phospho‐VEGFR2 Tyr 1175 (Cell Signaling Technology, 2478s, 1:1000); total VEGFR2 (Cell Signaling Technology, 2479s, 1:1000), HA‐tag (Cell Signaling Technology,

(17) Farrerol inhibits proliferation and migration of colorectal cancer via the VEGF signaling pathway: evidence from network pharmacology, molecular docking, molecular dynamics simulation, and in vitro experimentsFront PharmacolDecember 3, 2025Longhui Zhang, Qijing Xu, Guanduo Sun, Xiaokang Zhang, Jialong Xue, Chun Yao, Dechun Liu, Jingming ZhaiCo., Ltd. Anti-GAPDH (catalog number. 60004-1-Ig) was purchased from Proteintech Group, Inc. VEGFA (#2478), VEGFR2 (#2479), and p-VEGFR2 (#50661) were purchased from Cell Signaling Technology. 2.11 Cell cuture

(18) Engineering a heparin-mimetic biomaterial to promote tissue vascularizationCommunications BiologyNovember 14, 2025Linqing Li, Jinling Yang, Luba Perry, Jennifer L. Bays, Sangeeta N. Bhatia, Jeroen Eyckmans, Christopher S. Chen(phospho-proteins) and subjected to Western blot analysis using antibodies from Cell Signaling (pVEGFR2, 2478; VEGFR2, 2479; pERK1/2, #4370; ERK1/2, #4695; pAkt, #9271; Akt, #9272; and GAPDH, #5174). The blots

(19) APOA1 binding protein promotes lymphatic cell fate and lymphangiogenesis by relieving caveolae-mediated inhibition of VEGFR3 signalingNat CommunOctober 21, 2025Jun-Dae Kim, Surbhi Chaudhary, Weiqing Chen, Jonathan Astin, Philip S. Crosier, Pengchun Yu, John P. Cooke, Henry J. Pownall, Hugo J. Bellen, Nhat-Tu Le, et al.#ab41927; 1:500), GFP (Invitrogen #MA1-952; 1:1000), VEGFR2 (CST #2479L; 1:1000), pY1175-VEGFR2 (CST #2478S; 1:1000), 4G10 (CST #96215S; 1:1000), GRB2 (CST #3972S; 1:1000), and VEGFR3 (Santa cruz #SC321, 1:400).

(20) Maternal fish oil supplementation enhances nutrient transport in the placenta and milk biosynthesis in the mammary gland via the GPR120 signaling pathwayJournal of advanced researchOctober 1, 2025Li Q, Zhang Q, Su S, Yang S, Shao J, Guan W, Zhang S.Antibody,Cat,Company,Dilution ratio VEGFA,Proteintech,19003-1-AP,1000 VEGFR2,Proteintech,26415-1-AP,1000 p-VEGFR2,CST,2478S,1000 NF-κB,CST,4764S,1000 p-NF-κB,CST,3033S,1000 IκB,Abcam,ab32518,1000 p-IκB,Abcam,ab133462,1000 p38,Proteintech,66234-1-Ig,1000 p-P38,CST,4092S,1000 JNK,CST,9252S,1000 p-JNK,CST,4668S,1000 ERK,CST,9102S,1000 p-ERK,CST,9101S,1000 mTOR,CST,2983S,1000 p-mTOR,CST,5536S,1000 P70

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3/7/2017Phospho-VEGF Receptor 2 (Tyr1175) Antibody for Western Blots Adam KramerActivated VEGFR2 can be detected by the phosphorylation of tyrosine residue 1175. This can be detected by western blot using this antibody

Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb

Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb from Cell Signaling Technology

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