SynGAP (D20C7) Rabbit mAb 5539S from Cell Signaling Technology

Product Specs
ItemSynGAP (D20C7) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5539S
Quantity100 µl
ApplicationsW, IP
ReactivityM, R
TargetSynGAP
HostRabbit
NCBI Gene AliasesMRD5, RASA1, RASA5, SYNGAP
ClonalityMonoclonal
NCBI Full Gene Namesynaptic Ras GTPase activating protein 1
Research AreaNeuroscience
IsotypeIgG
CloneD20C7
Molecular Weight140

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contextsNat CommunDecember 9, 2025Preston Samowitz, Laszlo Radnai, Thomas Vaissiere, Sheldon D. Michaelson, Camilo Rojas, Ryan Mitchell, Murat Kilinc, Austin Edwards, Justin Shumate, Richard Hawkins, et al.analysis. Primary Antibodies Pan-SynGAP antibody (1:1000 for Immnoblotting, 1:500 for ICC)—Cell Signaling #5539 SynGAP-α2 antibody (1:1000)—Cell Signaling #56927 SynGAP-α1 antibody (50 ng/mL)—Cell Signaling Test

(2) Generation of humanized mouse models to support therapeutic development for SYNGAP1 and STXBP1 disordersbioRxivAugust 27, 2024Alex J. Felix, Taryn Wilson, Rani Randell, Nicolas Marotta, Keita Uchida, Michael J. Boland, Beverly L. Davidson, Benjamin L. Prossercontaining 0.1% Tween-20) was carried out overnight at 4 °C. Rabbit anti-SYNGAP1 (Cell Signaling Technology #5539S, 1:1000 dilution), rabbit anti-STXBP1 (Munc18–1, Cell Signaling Technology #13414S, 1:1000 dilution)

(3) Non-synaptic function of the autism spectrum disorder-associated gene SYNGAP1 in cortical neurogenesisNature NeuroscienceDecember 1, 2023Marcella Birtele, Ashley Del Dosso, Tiantian Xu, Tuan Nguyen, Brent Wilkinson, Negar Hosseini, Sarah Nguyen, Jean-Paul Urenda, Gavin Knight, Camilo Rojas, et al.competition assay Three consecutive organoids slices were used for analysis. Rabbit anti-SYNGAP1 (5539, Cell Signaling Technology) was diluted at a concentration of 1:200 in 0.3%TritonX-100 (Sigma, #T9284)

(4) The TMEM106B T186S coding variant increases neurite arborization and synaptic density in primary hippocampal neuronsFrontiers in NeuroscienceOctober 13, 2023Quynh Nguyen, Caleb A. Wood, Peter J. Kim, Joanna L. Jankowskydiluted in blocking solution [Bassoon (1:100, Enzo, ADI-VAM-PS003-D) and SynGAP (1:250, Cell Signaling, 5539S)] overnight at 4°C. Cells were washed in PBS then incubated in secondary antibody diluted in block solution

(5) Ebselen oxide and derivatives are new allosteric HER2 inhibitors for HER2-positive cancersMolecular oncologyOctober 1, 2023Blasquez L, Bouzinba-Segard H, Bourdoulous S, Faure C.Cell Signaling Technology (Danvers, MA, USA). Polyclonal anti‐ezrin (WB‐1 : 1000) was a kind gift of Dr C. Roy CNRS UMR 5539. Monoclonal antiphosphotyrosine (clone 4G10, WB‐1 : 100) was from Meditech (Waltham, MA, USA). Fluorescence‐conjugated

(6) Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophreniaiScienceMay 19, 2023Hsu YH, Pintacuda G, Liu R, Nacu E, Kim A, Tsafou K, Petrossian N, Crotty W, Suh JM, Riseman J, et al.AB_2798479 SRPK2,Fortis Life Sciences,Cat# A302-467A;RRID: AB_1944286 SYNGAP1,Cell Signaling Technology,Cat# 5539;RRID: AB_10694401 TCF4,GeneTex,Cat# GTX17912;RRID: AB_2887569 XRCC5,Abcam,Cat# ab80592;RRID: AB_1603758 XRCC6,Abcam,Cat#

(7) Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disordersCell GenomMarch 8, 2023Pintacuda G, Hsu YH, Tsafou K, Li KW, Martín JM, Riseman J, Biagini JC, Ching JKT, Mena D, Gonzalez-Lozano MA, et al.RRID: AB_2798017 SPTBN1,Abcam,Cat# ab72239; RRID: AB_1270902 SYNGAP,Cell Signaling Technology,Cat# 5539; RRID: AB_10694401 SCN2A,MilliporeSigma,Cat# MABN1586 TARDBP,Abcam,Cat# ab41881; RRID: AB_2200643 TLK2,Fortis

(8) Synaptoproteomic Analysis of the Prefrontal Cortex Reveals Spatio-Temporal Changes in SYNGAP1 Following Cannabinoid Exposure in Rat AdolescenceInternational Journal of Molecular SciencesDecember 31, 2022Johanna S. Qvist, Maria Scherma, Nitya Jayaram-Lindström, Walter Fratta, Denise B. Kandel, Eric R. Kandel, Paola Fadda, Philippe A. MelasRestore Fluorescent Western Blot Stripping Buffer (Thermo Fisher Scientific). The primary antibodies were: (i) SynGAP (D20C7) Rabbit mAb (#5539; Cell Signaling Technology, Inc., Danvers, MA, USA), (ii) Synaptophysin (D35E4) Rabbit mAb (#5461, Cell Signaling

(9) Disheveled binding antagonist of β-catenin 1 interacted with β-catenin and connexin 43 in human-induced pluripotent stem cells-derived cardiomyocytesBioengineeredMay 1, 2022Hou J, Huang S, Long Y, Feng K, Shang L, Zhou Z, Yue Y, Huang X, Chen G, Wu Z.Troponin I (1:200; Cell Signaling Technology, 13,083), Troponin T (1:200; Cell Signaling Technology, 5539), α-actinin (1:200; Cell Signaling Technology, 6487), NKX2.5 (1:200; Cell Signaling Technology, 8792),

(10) Activity dependent dissociation of the Homer1 interactomeScientific reportsFebruary 25, 2022Stillman M, Lautz JD, Johnson RS, MacCoss MJ, Smith SEP.clone K28/43, purchased from Biolegend #810401) and SynGAP (Cell Signaling Technology, clone D20C7, #5539), all at a 1:1000 dilution. Sample digestion The dried samples were resolubilized in 40 µL 0.1% PPS

(11) Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific statesCell reportsNovember 30, 2021Lautz JD, Tsegay KB, Zhu Z, Gniffke EP, Welsh JP, Smith SEP.,Bethyl Laboratories,A304-263A,AB_2620459 PIKE,Probe,DN8,Rockland,200-401-DN8,AB_2612161 SynGap,IP,D20C7,Cell Signaling,5539,AB_10694401 SynGap,Probe,PolyR19,Santa Cruz,sc-8572,AB_2200750 Ube3a,IP,H182,Santa Cruz,sc-25509,AB_639982 Ube3a,Probe,E4,Santa Cruz

(12) Homer1a regulates Shank3 expression and underlies behavioral vulnerability to stress in a model of Phelan-McDermid syndromeCell reportsNovember 16, 2021Lin R, Learman LN, Bangash MA, Melnikova T, Leyder E, Reddy SC, Naidoo N, Park JM, Savonenko A, Worley PF.,Cell Signaling Technology,"Cat# 3409, RRID:AB_1264242" SynGAP (D20C7) Rabbit mAb antibody,Cell Signaling Technology,"Cat# 5539, RRID:AB_10694401" Homer (D-3) antibody (long form Homer1),Santa Cruz Biotechnology,"Cat# sc-17842,

(13) Notch3 contributes to T-cell leukemia growth via regulation of the unfolded protein responseOncogenesisOctober 18, 2020Giuli MV, Diluvio G, Giuliani E, Franciosa G, Di Magno L, Pignataro MG, Tottone L, Nicoletti C, Besharat ZM, Peruzzi G, et al.biosystems, Newcastle, UK, Cat# NCL-L-LCA), anti-Notch3 (Santa Cruz Biotechnology, Dallas, TX, USA, Cat#5539) or anti-CHOP (Cell Signalling, Danvers, MA, USA, Cat#2895) antibodies. After washes, secondary biotinylated

(14) SYNGAP1 Controls the Maturation of Dendrites, Synaptic Function, and Network Activity in Developing Human NeuronsThe Journal of NeuroscienceOctober 7, 2020Nerea Llamosas et al.ChemiDoc imaging system (Bio-Rad). Membranes were blocked with 5% powdered milk in buffer and probed with pan-SynGAP (1:1000, #5539, Cell Signaling) or SynGAP-a2 (abcam, ab77235), overnight at 4°C and HRP-conjugated anti-rabbit antibody

(15) Dataset of complete genome assembly and analysis of mycobacterium tuberculosis strain SIT745/EAI1-MYSData in BriefAugust 1, 2020Mohammad Abdullah, Siti Suraiya, Suharni Mohamad, Azian Harun140,010,059","4482,059",65.6,3952,3,45,NC_015848.1 "M.paraseoulense strain JCM 16,952","6085,955",67.9,5539,3,46,NZ_AP022619.1 "M.parmense strain JCM 14,742","5952,912",68.4,5275,3,48,NZ_AP022614.1 "M.heidelbergense

(16) Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expressionNature communicationsJuly 9, 2020Lim KH, Han Z, Jeon HY, Kach J, Jing E, Weyn-Vanhentenryck S, Downs M, Corrionero A, Oh R, Scharner J, et al.hs.Ri.PCCA.13). For SynGAP, immunoblotting was carried out with 60 μg of lysate. Anti-SynGAP primary antibody (Cat# 5539, 1:1000, overnight at 4 °C) and secondary anti-rabbit HRP-conjugated (Cat# 7074, 1:5000, 1 h at RT)

(17) Quantitative phosphoproteomic analysis of the molecular substrates of sleep needNatureJune 13, 2018Zhiqiang Wang et al.[sc-805, Santa Cruz], anti-NMDAR1 [MAB363, EMD Millipore], anti-NMDAR2B [75–101, NeuroMab], anti-SynGAP [#5539, Cell Signaling], and anti-SIK3 C-term [a custom generated rabbit polyclonal antibody against the C-terminal

(18) Clustering the autisms using glutamate synapse protein interaction networks from cortical and hippocampal tissue of seven mouse modelsMolecular autismJanuary 1, 2018Brown EA, Lautz JD, Davis TR, Gniffke EP, VanSchoiack AAW, Neier SC, Tashbook N, Nicolini C, Fahnestock M, Schrum AG, et al.important in regulating synaptic binding “slots.”,1S,Multiple rare variants [90],Cell Signaling D20C7 Cat# 5539,SantaCruz polyclonal R19 Cat# sc8572 FYN,Fyn Kinase,"Associates with PSD95 and NMDARs, phosphorylates

(19) Role of transforming growth factor β‑1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic targetInternational journal of molecular medicineAugust 1, 2017Liu C, Wang Y, Li BS, Yang Q, Tang JM, Min J, Hong SS, Guo WJ, Hong L.

(20) Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP SignalingScientific reportsJuly 13, 2017Wilkinson B, Li J, Coba MP.#A304-262A), Kalirin (Millipore, catalog #07-0122), Syngap1 (Cell Signaling Technologies, catalog #5539), Rabbit IgG Isotype Control (ThermoFisher, catalog #06-6102) and GST (NeuroMab, catalog #75-148). All

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SynGAP (D20C7) Rabbit mAb

SynGAP (D20C7) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

Citations (23)

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SynGAP (D20C7) Rabbit mAb from Cell Signaling Technology

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