Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) 9654S from Cell Signaling Technology

Product Specs
ItemCleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated)
CompanyCell Signaling Technology
Price $477.00 Inquire
Catalog Number9654S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk
TargetCasp3 (Asp175) cleaved
HostRabbit
NCBI Gene AliasesCPP32, CPP32B, SCA-1
Conjugate/TagBiotin
ClonalityMonoclonal
NCBI Full Gene Namecaspase 3
Research AreaApoptosis
IsotypeIgG
Clone5A1E
Molecular Weight17, 19

Questions and Answers

Can this antibody be used to assess apoptosis in cancer cell lines?

Yes, the Cleaved Caspase-3 (Asp175) Rabbit mAb (Biotinylated) has been shown to be effective in detecting activated caspase-3, a key marker of apoptosis, in various cancer cell lines. For instance, it was utilized in studies to monitor apoptosis-related gene expression in endometrial cancer cells, demonstrating its relevance in apoptosis research ().

What species can this antibody react with?

This antibody is reactive with multiple species, including humans, mice, rats, and non-human primates. This broad reactivity makes it suitable for comparative studies across different model organisms.

How has this antibody been utilized in scientific research?

The Cleaved Caspase-3 antibody has been cited in numerous studies, including those investigating the mechanisms of apoptosis in various contexts, such as cancer treatment and neuroprotection. For example, it was used in Western blot analyses to evaluate apoptosis in retinal degeneration models (; ).

What is the recommended dilution for using this antibody in Western blot applications?

The recommended dilution for the Cleaved Caspase-3 antibody in Western blot applications is 1:1000, as indicated in various studies where it was employed to assess protein expression levels in apoptotic pathways (; ).

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Porphyromonas gingivalis secreted factors drive epithelial-mesenchymal transition (EMT) through gingipains and an H2S-mediated bacterial defense systemGut MicrobesDecember 31, 2026Michal Kazelnik, Rana Masri, Michal Caspi, Amnon Wittenstein, Ilan Tsarfaty, Elhanan Borenstein, Tal Caller, Konstantin Shatalin, Evgeny Nudler, Rina Rosin-ArbesfeldP-Yap(S397) (1:000, Cell signaling), mouse anti-Tubulin (1:10000; Sigma). CC3 (5A1E) Rabbit Monoclonal #9654 (1:1000, Cell Signaling), N-Cadherin (13A9) Mouse Monoclonal Antibody #14215 (1:600, Cell Signaling),

(2) Tetrandrine has protective role in myocardial ischemia/reperfusion injury via the TRPV2/Ca2+/calcineurin/NFAT axisHerzSeptember 10, 2025Wenbing Jiang, Lelin Jiang, Yiying Liu, Xiaoli Zhao, Shu’e Huang, Ying Liu, Huanghui Sun, Fanlu GuanChina" Bax,60267-1-Ig,"1:10,000","Proteintech, Wuhan, China" Survivin,66495-1-Ig,1:4000,"Proteintech, Wuhan, China" Cleaved Caspase‑3,9654S,1:1000,"Cell Signaling Technology, Boston, MA, USA" Calcineurin,16569-1-AP,1:1000,"Proteintech, Wuhan,

(3) Poloxamer 188 alleviates cerebral ischemia-reperfusion injury in mice by reducing mitochondrial and lysosomal membrane damageNeurosciences (Riyadh)July 1, 2025Hui Xu, Zhanhu Zhang, Xiaodong Tao, Ruirui Shi, Jian Xu, Xiaohua Zhang, Jinhua Guconcentration of 1%, and stored in the dark. Cyt c (4272), Cytochrome c oxidase (COX, 4850), Cleaved Caspase-3 (9654), Phospho-NF-kB p65 (3033) and NF-kb (8242) were sourced from Cell Signaling Technology (CST, Beverly,

(4) Sodium aescinate promotes apoptosis of pancreatic stellate cells and alleviates pancreatic fibrosis by inhibiting the PI3K/Akt/FOXO1 signaling pathwaysFrontiers in pharmacologyJanuary 1, 2025Wang QY, Xu BY, Wang Y, Lin YM, Zheng LF, Liu G, Li DZ, Jiang CS, Wang W, Zeng XP.(#15071),1:1000 Bax,Rb,IgG,Cell Signaling Technology (#5023),1:1000 Cleaved Caspase-3,Rb,IgG,Cell Signaling Technology (#9654),1:1000 Caspase-3,Rb,IgG,Cell Signaling Technology (#9662),1:1000 GAPDH,Ms,IgG1,Abcam (#ab8245),1:10000 Primary

(5) HSV-1 reactivation results in post-herpetic neuralgia by upregulating Prmt6 and inhibiting cGAS-STINGBrainJuly 5, 2024Erliang Kong, Tong Hua, Jian Li, Yongchang Li, Mei Yang, Ruifeng Ding, Haowei Wang, Huawei Wei, Xudong Feng, Chaofeng Han, et al.Cell Signaling Technology, #8592), anti-Cleaved caspase 3 antibody (1:1000, Cell Signaling Technology, #9654), anti-Cleaved Gasdermin D antibody (1:1000, Cell Signaling Technology, #36425), anti-cGAS antibody

(6) Decoding the neurotoxic effects of propofol: insights into the RARα-Snhg1-Bdnf regulatory cascadeAmerican Journal of Physiology-Cell PhysiologyJune 1, 2024Yuhai Xu, Xin Xin, Tianzhu TaoIdentifier (RRID): AB_331439; 1:1,000], rabbit anti-cleaved-caspase-3 (Cell Signaling Technology, cat. no. 9654; RRID: AB_10694088; 1:1,000), rabbit anti-RARα (Cell Signaling Technology, cat. no.62294; RRID: AB_2799625;

(7) Cathepsin V drives lung cancer progression by shaping the immunosuppressive environment and adhesion molecules cleavageAgingDecember 8, 2023Zhu L, Zeng Q, Wang J, Deng F, Jin S.Cat#13116S), HA-Tag (Cell Signaling Technology, Cat#3724S), Cleaved-Caspase 3 (Cell Signaling Technology, Cat#9654S), Cleaved-Caspase 7 (Cell Signaling Technology, Cat#8438S), Tubulin (Genetex, Cat#GTX112141), GAPDH

(8) USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stressNature CommunicationsOctober 13, 2023Kewei Qin, Shuhan Yu, Yang Liu, Rongtian Guo, Shiya Guo, Junjie Fei, Yuemeng Wang, Kaiyuan Jia, Zhiqiang Xu, Hu Chen, et al.1:1000), Tubulin (#3873, 1:1000), SP1 (#5931, 1:1000), HA-Tag (#5017, 1:1000), Cleaved-caspase 3 (CC3, #9654, 1:1000) and Flag-Tag (#8146, 1:1000) were purchased from Cell Signaling Technology (Danvers, MA, USA).

(9) Peptidase Inhibitor 16 Attenuates Left Ventricular Injury and Remodeling After Myocardial Infarction by Inhibiting the HDAC1-Wnt3a-β-Catenin Signaling AxisJournal of the American Heart AssociationMay 16, 2023Wang L, Du A, Lu Y, Zhao Y, Qiu M, Su Z, Shu H, Shen H, Sun W, Kong X.anti‐Bax (Bcl2‐Associated X protein) (catalog number 2772), anti‐cleaved caspase 3 (catalog number 9654), anti‐β‐catenin (catalog number 8480), and anti‐HDAC1 (catalog number 5356), which were all purchased

(10) Naringenin induces the cell apoptosis of acute myeloid leukemia cells by regulating the lncRNA XIST/miR-34a/HDAC1 signalingHeliyonMay 3, 2023Chao Wen, Xiaoliang Lu, Yingyin Sun, Qi Li, Jing Liao, Lin LiCell Signaling Technology, Cat.no.4223), cleaved caspase-3 (dilution, 1:1000; Cell Signaling Technology, Cat.no.9654), GAPDH (dilution, 1:1000; Cell Signaling Technology, Cat.no.5174), and the secondary antibody HRP-labeled

(11) PI3K/AKT/mTOR pathway and its related molecules participate in PROK1 silence-induced anti-tumor effects on pancreatic cancerOpen Life SciencesApril 10, 2023Feng Wang, Xiaogang Yan, Yongqiang Hua, Jianjun Song, Di Liu, Chun Yang, Fei Peng, Fuping Kang, Yongfeng HuiBax (CST; #2772; dilution, 1:1,000), Bcl-2 (CST; #4223; dilution, 1:1,000), cleaved caspase-3 (CST; #9654; dilution, 1:1,000), PI3K (Abcam; ab154598; dilution, 1:2,000), phospho-PI3K [Tyr458] (Abcam; ab278545;

(12) Etanercept ameliorates psoriasis progression through regulating high mobility group box 1 pathwaySkin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)April 1, 2023Li S, Li G, Li X, Wu F, Li L.anti‐Cyclin D1, cat. no. 55506, 1: 1000 dilution, CST; rabbit monoclonal anti‐cleaved caspase‐3, cat. no. 9654, 1: 1000 dilution, CST; rabbit monoclonal anti‐cleaved caspase‐9, cat. no. 7237, 1: 1000 dilution, CST;

(13) Psoralidin inhibits osteosarcoma growth and metastasis by downregulating ITGB1 expression via the FAK and PI3K/Akt signaling pathwaysChinese MedicineMarch 31, 2023Shengwen Cheng, Senrui Liu, Bowen Chen, Chengcheng Du, Pengcheng Xiao, Xuefeng Luo, Li Wei, Yiting Lei, Chen Zhao, Wei HuangCST), bcl-2 rabbit mAb (#4223, CST), bax rabbit mAb (#41,162, CST), cleaved caspase-3 rabbit mAb (#9654, CST), β-actin rabbit mAb (#4970, CST), anti-FAK (ab40794, Abcam), anti-p-FAK (phospho Y397) (ab81298,

(14) KCNK9 mediates the inhibitory effects of genistein on hepatic metastasis from colon cancerClinics (Sao Paulo)March 9, 2023Yuan Cheng, Yi Tang, Yiming Tan, Juan Li, Xuping ZhangCST Inc.), anti-Bcl-2 (#15071; CST Inc.), anti-caspase3 (#9662; CST Inc.), anti-cleaved caspase-3 (#9654; CST Inc.), anti-PARP (#9532; CST Inc.), anti-cleaved PARP (#9185; CST Inc.), anti-beta catenin (#ab32572;

(15) Xanthatin suppresses proliferation and tumorigenicity of glioma cells through autophagy inhibition via activation of the PI3K-Akt-mTOR pathwayPharmacology research & perspectivesFebruary 1, 2023Chen H, Zhu T, Huang X, Xu W, Di Z, Ma Y, Xue M, Bi S, Shen Y, Yu Y, et al.Sigma (St. Louis, MO, USA). The following antibodies were used: rabbit anti‐cleaved‐caspase‐3 (CST, 9654 s), rabbit anti‐cleaved‐PARP (CST, 5625 s), rabbit anti‐Beclin1 (CST, 3495 S), rabbit anti‐LC3 (Sigma,

(16) Folic acid Ameliorates the Declining Quality of Sodium Fluoride-Exposed Mouse Oocytes through the Sirt1/Sod2 PathwayAging and diseaseOctober 1, 2022Xiaoyuan Lin, Beibei Fu, Yan Xiong, Shiyao Xu, Jin Liu, Mohamed Y. Zaky, Dan Qiu, Haibo WuCell Signaling Technology, CST, Danvers, MA, USA), anti-Bax (89477, CST), anti- cleaved caspase 3 (9654, CST), and anti-cleaved caspase 9 (20750), anti-Sirt1 antibody (SAB570004, Sigma-Aldrich) and anti-Sod2

(17) Folic acid Ameliorates the Declining Quality of Sodium Fluoride-Exposed Mouse Oocytes through the Sirt1/Sod2 PathwayAging DisOctober 1, 2022Xiaoyuan Lin, Beibei Fu, Yan Xiong, Shiyao Xu, Jin Liu, Mohamed Y. Zaky, Dan Qiu, Haibo WuCell Signaling Technology, CST, Danvers, MA, USA), anti-Bax (89477, CST), anti- cleaved caspase 3 (9654, CST), and anti-cleaved caspase 9 (20750), anti-Sirt1 antibody (SAB570004, Sigma-Aldrich) and anti-Sod2

(18) microRNA-140-3p protects hippocampal neuron against pyroptosis to attenuate sevoflurane inhalation-induced post-operative cognitive dysfunction in rats via activation of HTR2A/ERK/Nrf2 axis by targeting DNMT1Cell death discoveryJune 16, 2022Wu Z, Tan J, Lin L, Zhang W, Yuan W.Abcam), speck-like protein (ASC, 1:500, sc-271054, Santa Cruz Biotechnology), Cleaved caspase-3 (1:1000, #9654, CST), Cleaved caspase-1 (1:1000, PA5-105049, Invitrogen), Cleaved N-terminal Gasdermin-D (GSDMD, 1:1000,

(19) The activation of SIRT3 by dexmedetomidine mitigates limb ischemia-reperfusion-induced lung injuryAnnals of translational medicineMarch 1, 2022Wang L, Ding Y, Bai Y, Shi J, Li J, Wang X.(A0674), HO-1 (A19062), Bcl-1 (A0208) and Bax (A1968) (ABclona, Wuhan, China); cleaved-caspase 3 (#9654) and Cytochrome c (#11940) (Cell Signaling Technology; Danvers, MA, USA); COX IV (GTX49132); GAPDH (60004-1-Ig)

(20) Protective Effect of Curcuma Extract in an Ex Vivo Model of Retinal Degeneration via Antioxidant Activity and Targeting the SUMOylationOxidative medicine and cellular longevityJanuary 1, 2022Hassanzadeh K, Vahabzadeh Z, Bucarello L, Dragotto J, Corbo M, Maccarone R, Feligioni M.(Santa Cruz Biotechnology, Milan, Italy Cat#C-20 sc31984), anti-cleaved caspase 3 (Cell Signaling, Danvers, MA, USA. cat #9654), anti-p-c-Jun (Cell Signaling, Danvers, MA, USA. cat#9164), anti-c-Jun (Cell Signaling, Danvers, MA,

Be the First to Write a Review!

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated)

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (78)

Reviews

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map