Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate) 5742S from Cell Signaling Technology

Product Specs
ItemRabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5742S
Quantity100 µl
HostRabbit
Conjugate/TagPE
ClonalityMonoclonal
ApplicationsFC-FP, FC-L
Research AreaRelated Products
TargetRabbit IgG isotype control
IsotypeIgG
CloneDA1E

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infectionPLoS pathogensAugust 1, 2023Phan QT, Solis NV, Cravener MV, Swidergall M, Lin J, Huang MY, Liu H, Singh S, Ibrahim AS, Mazzone M, et al.Cell Signaling Technologies) and control slides were stained with rabbit IgG conjugated with phycoerthrin (#5742, Cell Signaling Technologies). The slides were imaged by confocal microscopy, and z-stacks were combined

(2) Development of innate immune memory by non-immune cells during Staphylococcus aureus infection depends on reactive oxygen speciesFrontiers in ImmunologyMay 31, 2023Emmanuel Chaumond, Sandrine Peron, Nathalie Daniel, Yann Le Gouar, Éric Guédon, David L. Williams, Yves Le Loir, Gwénaël Jan, Nadia BerkovaPBS/2%BSA (1:50) for 1 h. An isotype control antibody, which is not directed against any known antigen (#5742, Rabbit (DA1E) mAb IgG XP® Isotype Control, PE Conjugate, Cell Signaling Technology Europe, Leiden,

(3) Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infectionbioRxiv : the preprint server for biologyFebruary 23, 2023Phan QT, Solis NV, Cravener MV, Swidergall M, Lin J, Huang MY, Liu H, Singh S, Ibrahim AS, Mazzone M, et al.Cell Signaling Technologies) and control slides were stained with rabbit IgG conjugated with phycoerthrin (#5742, Cell Signaling Technologies). The slides were imaged by confocal microscopy, and z-stacks were combined

(4) Engineering human stem cell-derived islets to evade immune rejection and promote localized immune toleranceCell Reports MedicineJanuary 17, 2023Dario Gerace, Quan Zhou, Jennifer Hyoje-Ryu Kenty, Adrian Veres, Elad Sintov, Xi Wang, Kyle R. Boulanger, Hongfei Li, Douglas A. MeltonIgG2a (Biolegend, 400213), APC rat IgG1 (Biolegend, 401903), PE rabbit IgG (Cell Signaling technology, 5742S) and goat anti-mouse 488 (Thermofisher, A-11001, 1:300) served as isotype controls. All antibodies were

(5) Obesity alters the mouse endometrial transcriptome in a cell context-dependent mannerReproductive Biology and EndocrinologyNovember 24, 2022Mike R. Wilson, Hilary Skalski, Jake J. Reske, Marc Wegener, Marie Adams, Galen Hostetter, Hanne M. Hoffmann, Jamie J. Bernard, Victoria L. Bae-Jump, Jose M. Teixeira, et al.PE-labeled anti-Vimentin (Cell Signaling, cat# 12,020) or PE-labeled rabbit IgG (Cell Signaling, cat# 5742) for one hour at room temperature. Purity of fixed cells were confirmed by flow cytometry. Epithelia

(6) The mammalian SKIV2L RNA exosome is essential for early B cell developmentScience immunologyJune 3, 2022Yang K, Han J, Gill JG, Park JY, Sathe MN, Gattineni J, Wright T, Wysocki C, de la Morena MT, Yan N.Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Cell Signaling, Cat # 5763), PE Rabbit (DA1E) mAb IgG XP® Isotype Control (Cell Signaling, Cat # 5742), R-Phycoerythrin AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgM, μ chain specific (Jackson ImmunoResearch,

(7) Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liverScience advancesMarch 18, 2022Lee Y, DiMaulo-Milk E, Leslie J, Ding L.(Cell Signaling Technology, 5387), rabbit immunoglobulin G XP Isotype Control PE (Cell Signaling Technology, 5742), and anti-γH2AX (clone 2F3; BioLegend, 613405). After staining, cells were washed in Perm/Wash buffer

(8) Klf4 methylated by Prmt1 restrains the commitment of primitive endodermNucleic acids researchFebruary 28, 2022Zuo ZY, Yang GH, Wang HY, Liu SY, Zhang YJ, Cai Y, Chen F, Dai H, Xiao Y, Cheng MB, et al.Cell Signaling Technology), Isotype control APC (Cat#130-104-615, Miltenyi Biotec) and Isotype control PE (Cat#5742, Cell Signaling Technology). The stained cells were analyzed on a C6 flow cytometer (BD). Data were

(9) Targeting Glycolysis in Alloreactive T Cells to Prevent Acute Graft-Versus-Host Disease While Preserving Graft-Versus-Leukemia EffectFrontiers in immunologyJanuary 1, 2022Huang Y, Zou Y, Jiao Y, Shi P, Nie X, Huang W, Xiong C, Choi M, Huang C, Macintyre AN, et al.addition of unconjugated primary antibodies. For isotype controls, Rabbit IgG XP (R)-PE (CST, catalog 5742S), mouse IgG1k-PE (BD Pharmingen, clone MOPC-21), Rabbit IgG (Abcam, ab37415), mouse IgG1k (Abcam, clone

(10) Sustained microglial activation in the area postrema of collagen-induced arthritis miceArthritis Research & TherapyDecember 1, 2021Takayuki Matsushita, Kazuhiro Otani, Yohsuke Oto, Yukari Takahashi, Daitaro Kurosaka, Fusao KatoStudies of Hybridoma Bank, Iowa University, Iowa City, IA, USA). Rabbit nonspecific IgG (20 μg/μL, 5742S; Cell Signaling Technology, Danvers, MA, USA) was used as an isotype control for the anti-Iba-1 antibody.

(11) The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in miceeLifeNovember 11, 2021Lehrke MJ, Shapiro MJ, Rajcula MJ, Kennedy MM, McCue SA, Medina KL, Shapiro VS.interest) Antibody,"Rabbit IgG Isotype Control PE (Rabbit monoclonal, DA1E)",Cell Signaling Technology,Cat#:5742; RRID:AB_10694219,FC (concentration matched antibodies of interest) Antibody,"Rabbit IgG Monoclonal

(12) An IRF4–MYC–mTORC1 Integrated Pathway Controls Cell Growth and the Proliferative Capacity of Activated B Cells during B Cell Differentiation In VivoThe Journal of ImmunologyOctober 1, 2021Dillon G. Patterson, Anna K. Kania, Madeline J. Price, James R. Rose, Christopher D. Scharer, Jeremy M. BossIgG XP Isotype-Alexa Fluor 647 (Cell Signaling; 2985), Rabbit mAb IgG XP Isotype-PE (Cell Signaling; 5742), Zombie Yellow Fixable Viability Kit (Biolegend; 423104), Zombie NIR Fixable Viability Kit (Biolegend;

(13) TCF-1 controls T_reg cell functions that regulate inflammation, CD8⁺ T cell cytotoxicity and severity of colon cancerNature immunologySeptember 1, 2021Osman A, Yan B, Li Y, Pavelko KD, Quandt J, Saadalla A, Singh MP, Kazemian M, Gounari F, Khazaie K.(dilution: 1/100; clone: D57.2.2E; cat: 5316S) and rabbit IgG-PE (dilution: 1/200; clone: DA1E; cat: 5742S; both from Cell Signaling Technology), FITC-conjugated STAT5 phosphorylated at Tyr694 (dilution: 1μg/test;

(14) Dysregulated NF-κB-Dependent ICOSL Expression in Human Dendritic Cell Vaccines Impairs T-cell Responses in Patients with MelanomaCancer immunology researchDecember 1, 2020Maurer DM, Adamik J, Santos PM, Shi J, Shurin MR, Kirkwood JM, Storkus WJ, Butterfield LH.Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate; Cell Signaling # 3452S), Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate; Cell Signaling #5742). The following antibodies were used for ImageStream Analysis: NF-κB p65 (D14E12) Rabbit mAb (Alexa

(15) A human ESC-based screen identifies a role for the translated lncRNA LINC00261 in pancreatic endocrine differentiationeLifeAugust 3, 2020Bjoern Gaertner et al.RRID:AB_10717121",Flow cytometry (5 ul per test) Antibody,IgG-PE (Rabbit monoclonal),Cell Signaling Technology,"Cat# 5742S, RRID:AB_10694219",Flow cytometry isotype control antibody (1:50) Antibody,"IgG1, κ antibody (Mouse

(16) Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP)Methods in Molecular BiologyAugust 13, 2019Lindsey M. Ludwig, Katrina L. Maxcy, James L. LaBelleIsotype Control Alexa(R)647 CST 2985S.(b)Rabbit (DA1E) mAb IgG XP(R) Isotype Control (PE Conjugate) CST 5742S.(c)PE Hamster Anti-Mouse BCL-2 Isotype Control (in kit listed above). Optional: Secondary antibodies

(17) A Stromal Niche Defined by Expression of the Transcription Factor WT1 Mediates Programming and Homeostasis of Cavity-Resident MacrophagesImmunityJuly 16, 2019Buechler MB, Kim KW, Onufer EJ, Williams JW, Little CC, Dominguez CX, Li Q, Sandoval W, Cooper JE, Harris CA, et al.with GATA6 (PE conjugate [50ug/mL], CST cat. 26452S at 1:50; or Rabbit isotype PE [100ug/mL], CST cat. 5742S 1:100) for 30 minutes on ice. Live cells were identified by washing after Fc block and incubation with

(18) Quantitative Difference in PLZF Protein Expression Determines iNKT Lineage Fate and Controls Innate CD8 T Cell GenerationCell reportsMay 28, 2019Park JY, DiPalma DT, Kwon J, Fink J, Park JH.,Cell Signaling Technology,Cat#: 13835S; RRID:AB_ 2228008 PE isotype control (clone DA1E),Cell Signaling Technology,Cat#: 5742S; RRID:AB_1550038 Alexa Fluor 647 anti-PLZF (clone R17-809),BD Biosciences,Cat#: 563490; RRID:AB_2738238 FITC

(19) Mammalian cell growth dynamics in mitosiseLifeMay 7, 2019Miettinen TP, Kang JH, Yang LF, Manalis SR.isotype specific controls (Rabbit mAb IgG, conjugated to PE or Alexa 647, Cell Signaling Technology, #5742S) in PBS solution containing 5% BSA. All antibodies were used at the concentration recommended by supplier.

(20) LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltrationNatureOctober 1, 2018Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, He L, Chen Y, Chen H, Luo W, et al.anti-Arginase-1 (D4E3M) (Cell signaling, Cat#93668, 1:100), rabbit IgG Isotype control-PE (Cell signaling, Cat#5742, 1:100), mouse IgG Isotype control-PE (eBioscience, m2a-15F8, 1:100) and anti-rabbit IgG-PE (Jackson Immunoresearch

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Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate)

Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate) from Cell Signaling Technology

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