MDA-5 (D74E4) Rabbit mAb 5321S from Cell Signaling Technology

Product Specs
ItemMDA-5 (D74E4) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5321S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M
TargetMDA5
HostRabbit
NCBI Gene AliasesAGS7, Hlcd, IDDM19, IMD95, MDA-5, MDA5, RLR-2, SGMRT1
ClonalityMonoclonal
NCBI Full Gene Nameinterferon induced with helicase C domain 1
Research AreaImmunology and Inflammation
IsotypeIgG
CloneD74E4
Molecular Weight135

Questions and Answers

Can this antibody be used to study the role of MDA5 in viral infections?

Yes, the MDA-5 (D74E4) Rabbit mAb has been shown to be effective in studies investigating the role of MDA5 in type I interferon responses during viral infections, particularly with positive-sense, single-stranded RNA viruses like the encephalomyocarditis virus. It has been demonstrated that MDA5 is crucial for the induction of type I interferon responses, which are essential for antiviral immunity ().

Is this antibody suitable for use in Western blotting applications?

The MDA-5 (D74E4) Rabbit mAb has been utilized in Western blotting, with a recommended dilution of 1:1000, as evidenced in various studies. It has been successfully employed to detect MDA5 protein levels in different experimental setups, confirming its utility in this application (, ).

What species can this antibody detect, and is it effective in mouse models?

This antibody is reactive with both human and mouse MDA5 proteins, making it suitable for studies involving these species. Its effectiveness in mouse models has been highlighted in research examining the immune response to viral infections, where MDA5 plays a significant role in mediating type I interferon production (, ).

Are there any known interactions of MDA5 that this antibody can help elucidate?

Yes, the MDA-5 (D74E4) Rabbit mAb can be used to explore interactions with other proteins involved in the innate immune response, such as ADAM9, which has been shown to promote MDA5 oligomerization and activation in response to viral RNA stimulation. This highlights the antibody's potential in dissecting complex signaling pathways in immune responses ().

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) WNT inhibition activates interferon stimulated gene expression by alleviating epigenetic repression of endogenous retrovirusesScientific ReportsMarch 5, 2026Courtney M. Williams, Jessica Harper Calderon, Varenka Rodriguez DiBlasi, Louis Jinrui Liu, Samer Nuwayhid, Hannah Cevasco, Wei Wang, Rekha Soni, Christina Adler, David D’Ambrosio, et al.ISG15(2758), GBP2 (53311), phospho-TBK1/NAK (5483), TBK1/NAK (3504), phospho-IRF-3 (37829), MDA-5 (5321), RIG-I (3743), P-STING (19781),STING (13647), LSD1 (2184), DNMT1 (5032), EZH2 (5246), ERK (4695), phospho-ERK

(2) USP30-AS1 functions as a post-transcriptional amplifier of interferon signalling to drive autoimmune pathogenesisCell & BioscienceFebruary 26, 2026Jie Pan, Min-Yi Feng, Tian Xia, Shuai Jiang, Peng-Peng Zhu, Xi-Ping Yu, Zhi-Hui Su, Yu Liu, Jia-Qi Wu, Ming Zhao, et al.Thermo Fisher Scientific; anti-USP30 (sc-515235) and anti-β-Actin (sc-47778) were from Santa Cruz Biotechnology; anti-MDA5 (5321S) and anti-PKR (12297S) were from Cell Signalling Technology; anti-GAPDH (10494-1-AP) was from Proteintech Group

(3) The human antibacterial factor APOL3 couples lysosomal damage to mitochondrial DNA efflux and type I IFN inductionMol CellFebruary 24, 2026Dominic A. Ritacco, Hamna Shahnawaz, Antonia Oduguwa, Jacob Hawk, Brianna Vizcaino, Donna L. Farber, Ryan G. Gaudetanti-TFAM,Cell Signaling,7495 Rabbit anti-RIG-I,Cell Signaling,3743 Rabbit anti-MDA-5,Cell Signaling,5321 anti-HA.11 epitope tag,Biolegend,16B12 Human anti-cardiolipin,USBiological,C1375 Rabbit anti-PLSCR3,Proteintech,28028-1-AP Goat

(4) RNF20 dynamically regulates RIG-I and MDA5 transcription and degradation via nucleocytoplasmic translocation to balance antiviral signalingPLOS PathogensFebruary 19, 2026Jie Wang, Qiuju Liu, Shurui Zhao, Qi Shao, Feiyu Fu, Kehui Zhang, Jingjiao Ma, Zhaofei Wang, Yaxian Yan, Hengan Wang, et al.sc-517358), anti-RIG-I (Cell Signaling Technologies, 3743), anti-MDA5 (Cell Signaling Technologies, 5321), anti-MAVS (Cell Signaling Technologies, 3993), anti-TBK1 (Cell Signaling Technologies, 3504), anti-p-TBK1

(5) N6-methyladenosine within transmissible gastroenteritis virus genomic RNA inhibits its replication via efficient recognition by RNA sensor RIG-IJournal of VirologyFebruary 17, 2026Jianing Chen, Shengyu Lin, Qianzi Liu, Mengling Gao, Zemei Wang, Jiao Tang, Yaru Cui, Chen Tan, Guangliang Liu(3743, CST), rabbit anti-IRF3 mAb (4302, CST), rabbit anti-pIRF3 mAb (29047, CST), rabbit anti-MDA5 mAb (5321, CST). Virus titration For virus titration, PK-15 cells were seeded into 96-well plates. Supernatants

(6) IGFBP-6 regulates breast cancer cell cycle progression by promoting exit out of G1Journal of Biological ChemistryFebruary 1, 2026Francisco J. Lariz, Shayla Hernandez, Diana C. Bautista-Tovar, Kevin D. HoustonAntibodies used are as follows: IGFBP-6 (#ab219560, Abcam, Waltham, MA), MX1 (#37849 Cell Signaling), MDA5 (#5321 Cell Signaling), IRF9 (#76684 Cell Signaling), ISG15 (#2758 Cell Signaling), Phospho-STAT1 (Y701) (#7649

(7) The lysosomal LAMTOR-Rag complex functions as a checkpoint for antiviral interferon productionThe EMBO JournalJanuary 22, 2026Zeming Feng, Lulu Wang, Shujun Chen, Sihan Cao, Miao Lei, Xiuzhen Yang, Kaixiong Ma, Shi Yu, Huina Hu, Kaixuan Zheng, et al.(D4V4S) ISG15 (IB 1:1000),Cell Signaling Technology,#2743 MDA-5 (IB 1:1000),Cell Signaling Technology,#5321 (D74E4) MyD88 (IB 1:1000),Cell Signaling Technology,#4283 (D80F5) TLR3 (IB 1:1000),Cell Signaling Technology

(8) RNA innate immunity constitutes a barrier for interspecies chimerismCellJanuary 8, 2026Yingying Hu, Hai-Xi Sun, Masahiro Sakurai, Zhou Luo, Amanda E. Jones, Tianlei Cheng, Jia Huang, Lizhong Liu, Canbin Zheng, Jie Li, et al.anti- CLEAVED CASPASE-3,Cell Signaling Technology,#9661 Rabbit monoclonal anti-MDA5,Cell Signaling Technology,#5321 Rabbit monoclonal anti-RIG-I,Cell Signaling Technology,#3743 Rabbit monoclonal anti-Vinculin,Cell Signaling Technology

(9) Protective IFIH1 variant reduces immune-mediated islet stress and dysfunction in a type 1 diabetes genetic backgroundbioRxivJanuary 3, 2026Daniel A. Veronese-Paniagua, Cameron Banks, Kameron Bradley, Noyonika Mukherjee, Sarah E. Gale, Kate E. Hinshaw, Amy M. Meacham, Opeoluwa F. Iwaloye, Hubert M. Tse, Clayton E. Mathews, et al.was determined by BCA (Thermo Fisher, 23227). Dynabeads were linked to MDA5 antibody (Cell Signaling, 5321), which was diluted at 1:100, and rotated for 10 min at room temperature. 500μg protein was added to

(10) Nonsense-mediated mRNA decay inhibits TRAF6-dependent anti-tumor immunity in colorectal cancerCell Reports MedicineDecember 16, 2025Yanmei Wang, Zhuo Wang, Chaoqun Wang, Yan Wu, Jiaqiu Li, Lifeng Feng, Minyan Hao, Hongchuan Jin, Jia Zhou, Xian WangRRID:AB_2799375 Anti-RIG-I,Cell Signaling Technology,Cat# 3743; RRID:AB_2269233 Anti-MDA-5,Cell Signaling Technology,Cat# 5321; RRID:AB_10694490 Anti-TRAF3,ABclonal,Cat# A3094; RRID:AB_2764895 Anti-TRAF6,Abcam,Cat# ab40675; RRID:AB_778573 Anti-Flag-tag,Sigma-Aldrich,Cat#

(11) Nef stabilizes actin to prevent HIV-1 sensing by RIG-I-like receptorsNat CommunDecember 7, 2025Alexandre Laliberté, Caterina Prelli Bozzo, Dhiraj Acharya, Aurora De Luna, Maximilian Hirschenberger, Junji Zhu, Meta Volcic, Bettina Stolp, Cristina M. Rodriguez-Quinteros, Oliver T. Fackler, et al.anti-MAVS (1:1000, Cell Signaling Technology), rabbit anti-MDA5 (1:1000, Cell Signaling Technology, Cat#5321), rabbit anti-pS88 MDA514, mouse anti-RIG-I (1:1000, Adipogen, AG-20B-0009-C100), Rabbit anti-AU1, (1:1000,

(12) Hypoxia Induces Immunosuppression by Silencing STING in CancerCancer ResearchDecember 1, 2025Yuhong Lu, Annali M. Yurkevicz, Yanfeng Liu, Peter M. Glazer(KDM1A) (Cat#: 2139, Cell signaling); anti-JARID1A (KDM5A) (Cat#: 3876, Cell signaling); MDA5(Cat#: 5321, Cell signaling); anti-HIF1α (Cat#: 36169, Cell signaling); anti-cGas (Cat#: 15102, Cell signaling). Quantitative real-time PCR

(13) Impaired hematopoiesis and embryonic lethality at midgestation of mice lacking both lipid transfer proteins VPS13A and VPS13CPLOS BiologySeptember 16, 2025Peng Xu, Rubia Isler Mancuso, Marianna Leonzino, Caroline J. Zeiss, Diane S. Krause, Pietro De CamilliRRID:AB_2918183), VPS13C (custom [34], Proteintech, RRID: AB_3711218), MDA5 (Cell Signaling Technology Cat# 5321, RRID:AB_10694490), RIGI (Proteintech Cat# 25068-1-AP, RRID:AB_2879881), IFITM3 (Cell Signaling Technology

(14) Activation of APOBEC3 cytidine deaminases and endogenous retroviruses is integrated by MUC1-C in NSCLC cellsCell Death DiscovAugust 8, 2025Naoki Haratake, Shinkichi Takamori, Hideko Isozaki, Keisuke Shigeta, Chie Kikutake, Hiroki Ozawa, Atrayee Bhattacharya, Ayako Nakashoji, Mikita Suyama, Tomoyoshi Takenaka, et al.(#8478, 1:1000; CST), anti-cGAS (#15102, 1:1000; CST), anti-STING (#13647, 1:1000; CST), anti-MDA5 (#5321, 1:1000; CST) and anti-RIG-I (#3743, 1:1000; CST) as described [22, 32, 33]. Chromatin immunoprecipitation Chromatin

(15) Unlocking the Puzzle of Mammalian Transfection: The Role of the RNA-sensing-Mediated Interferon Response in the Cellular Defense Against Foreign DNA IntrusionInternational Journal of Biological SciencesJune 9, 2025Xiaoyu Li, Yicen Zhou, Jie Wei, Wei Sun, Ligang Fan, Jian Yananti-IRF3 ( 4302), anti-p-IRF3 (Ser396, 4947), anti-TBK1 (3504), anti-p-TBK1 (Ser172, 5483), anti-MDA5 (5321), anti-RIGI (3743), and anti-IRF7 (4920) from Cell Signaling Technology; anti-STING (19851), GAPDH (10494),

(16) SLIRP amplifies antiviral signaling via positive feedback regulation and contributes to autoimmune diseasesCell ReportsMay 27, 2025Doyeong Ku, Yewon Yang, Youngran Park, Daesong Jang, Namseok Lee, Yong-ki Lee, Keonyong Lee, Jaeseon Lee, Yeon Bi Han, Soojin Jang, et al.,Cell Signaling Technology,Cat# 4642; RRID: AB_2224542 Rabbit monoclonal MDA5 (D74E4),Cell Signaling Technology,Cat# 5321; RRID: AB_10694490 Mouse monoclonal GAPDH (6C5),Santa Cruz Biotechnology,Cat# sc-32233; RRID: AB_627679 Rabbit

(17) The antibacterial factor APOL3 couples lysosomal damage to mitochondrial DNA efflux and type I IFN inductionbioRxivMay 22, 2025Dominic A. Ritacco, Hamna Shahnawaz, Antonia Oduguwa, Jacob Hawk, Brianna Vizcaino, Donna Farber, Ryan G. Gaudetanti-TFAM,Cell Signaling,7495 Rabbit anti-RIG-I,Cell Signaling,3743 Rabbit anti-MDA-5,Cell Signaling,5321 anti-HA.11 epitope tag,Biolegend,16B12 Human anti-cardiolipin,USBiological,C1375 Rabbit anti-PLSCR3,Proteintech,28028-1-AP Goat

(18) LINE-1 ORF1p Mimics Viral Innate Immune Evasion Mechanisms in Pancreatic Ductal AdenocarcinomaCancer DiscoveryMay 2, 2025Eunae You, Bidish K. Patel, Alexandra S. Rojas, Siyu Sun, Patrick Danaher, Natalie I. Ho, Ildiko E. Phillips, Michael J. Raabe, Yuhui Song, Katherine H. Xu, et al.anti-γH2AX (S139; Cell Signaling Technology, cat. #9718), anti-MDA5 (Cell Signaling Technology, cat. #5321), anti-RIG-I (Cell Signaling Technology, cat. #3743), anti-MAVS (Cell Signaling Technology, cat. #3993),

(19) SAMD9 senses cytosolic double-stranded nucleic acids in epithelial and mesenchymal cells to induce antiviral immunityNature communicationsApril 22, 2025Hou G, Beatty W, Ren L, Ooi YS, Son J, Zhu Y, Sheng Q, Huang W, Li D, Liu C, et al.SAMD9 (Sigma-Aldrich #HPA021319, 1:5000), RIG-I (Cell signaling #3743, 1:1000), MDA5 (Cell signaling #5321, 1:1000), MAVS (Cell signaling #24930, 1:1000), STING (Cell signaling #13647, 1:1000), TBK1 (Cell signaling

(20) The African swine fever virus gene MGF_360-4L inhibits interferon signaling by recruiting mitochondrial selective autophagy receptor SQSTM1 degrading MDA5 antagonizing innate immune responsesmBioApril 9, 2025Sun H, Yang J, Zhang Z, Wu M, Tian Z, Liu Y, Zhang X, Zhong J, Yang S, Chen Y, et al.(CST, 13987), and mouse anti-Myc (CST, 2276); rabbit anti-Rig-I (CST, 3743), rabbit anti-MDA5 (CST, 5321), rabbit anti-MAVS (CST, 24930), rabbit anti-TRAF3 (CST, 33640), rabbit anti-IKKε (CST, 96794), rabbit

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6/5/2021Anti-MDA5 Antibody Works Great for Western Blot Liyong Z.To examine the expression levels of MDA5 in mouse brain and spleen, tissues were collected and lysed with the buffer [1% SDS, 10 mmol/L Tris-Cl (pH 7.6), 20 µg/µL aprotinin, 20 µg/µL leupeptin, and 1 ... Read Review

MDA-5 (D74E4) Rabbit mAb

MDA-5 (D74E4) Rabbit mAb from Cell Signaling Technology

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MDA-5 (D74E4) Rabbit mAb from Cell Signaling Technology

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