HMGA2 Antibody 5269S from Cell Signaling Technology

Product Specs
ItemHMGA2 Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number5269S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R
TargetHMGA2
HostRabbit
NCBI Gene AliasesBABL, HMGI-C, HMGIC, LIPO, SRS5, STQTL9
NCBI Full Gene Namehigh mobility group AT-hook 2
ClonalityPolyclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight18

Questions and Answers

Can this antibody be used to study the role of HMGA2 in cancer progression?

Yes, the HMGA2 antibody has been utilized in various studies to investigate its role in cancer. For instance, it has been shown to promote vasculogenic mimicry and tumor aggressiveness in gastric carcinoma by upregulating Twist1, indicating its involvement in cancer progression and metastasis ().

What applications is this antibody suitable for in experimental protocols?

The HMGA2 antibody is suitable for Western Blotting (WB) and Immunoprecipitation (IP). It has been effectively used in these applications to analyze HMGA2 expression and its interactions with other proteins in various studies (, ).

Is there evidence of this antibody being used in studies related to chemoresistance?

Yes, the HMGA2 antibody has been referenced in studies examining chemoresistance. For example, it was involved in research that identified HMGA2 as a target in the context of triple-negative breast cancer, where its expression was linked to chemoresistance mechanisms ().

What species does this antibody react with, and is it effective for human samples?

The HMGA2 antibody is reactive with mouse, rat, and human samples, making it suitable for studies involving these species, particularly in human cancer research where HMGA2 plays a significant role ().

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) HMGA2 and protein leucine methylation drive pancreatic cancer lineage plasticityNat CommunMay 26, 2025Stephanie Dobersch, Naomi Yamamoto, Aidan Schutter, Sarah M. Cavender, Tess M. Robertson, Nithya Kartha, Annie N. Samraj, Ben Doron, Lisa A. Poole, Cynthia L. Wladyka, et al.ECL (Biorad). The following primary antibody dilutions were used: anti-HMGA2 (1:1000, Cell Signaling #5269), H3 (1:5000, Abcam #ab1791), anti-FLAG (1:1000, Sigma Aldrich #F1804), anti-IMP1 (1:1000, Cell Signaling

(2) Non-genotoxic carcinogens (TPA and mezerein) activate tumourous transformation through miR let-7-mediated Hmga2 expression in Bhas42 cellsEnvironmental EpigeneticsMarch 3, 2025Moon Yi Ko, Euijun Min, Minjeong Kim, Heejin Park, Sumi Jang, Younhee Kim, Byoung-Seok Lee, Sung-Ae Hyun, Minhan Kaand incubated overnight at 4°C with primary antibodies against Ezh2 (#5246, Cell Signaling), Hmga2 (#5269, Cell Signaling), and β-actin (SC-517582, Santa Cruz). The PVDF membrane was then probed with appropriate

(3) A glucose-enriched lung pre-metastatic niche triggered by matrix stiffness-tuned exosomal miRNAs in hepatocellular carcinomaNature communicationsFebruary 18, 2025Zhao Y, Yu H, Li J, Qian J, Li M, Zhang X, Wang M, Wang Y, Dong Y, You Y, et al.1:1000), Albumin (Abcam, ab207327, 1:2000), Cytochrome C (Proteintech, 66264-1-Ig, 1:5000), HMGA2 (CST, 5269, 1:1000), Rb (Abcam, ab181616, 1:1000), E2F1 (Invitrogen, 32-1400, 1:500), HDAC1 (Proteintch, 66085-1-Ig,

(4) HMGA2 Expression Predicts Subtype, Survival, and Treatment Outcome in Pancreatic Ductal AdenocarcinomaClinical Cancer ResearchFebruary 17, 2025Naomi Yamamoto, Stephanie Dobersch, Ian Loveless, Annie N. Samraj, Gun Ho Jang, Miki Haraguchi, Liang-I. Kang, Marianna B. Ruzinova, Kiran R. Vij, Jacqueline L. Mudd, et al.comprised antibodies for FAP (Abcam Cat# ab53066, RRID:AB_880077), HMGA2 (Cell Signaling Technology Cat# 5269, RRID:AB_10694917), CK17 (Abcam Cat# ab109725, RRID:AB_10889888), CD45 (Abcam Cat# ab40763, RRID:AB_726545),

(5) HMGA2 Expression Predicts Subtype, Survival, and Treatment Outcome in Pancreatic Ductal AdenocarcinomaClin Cancer ResFebruary 17, 2025Naomi Yamamoto, Stephanie Dobersch, Ian Loveless, Annie N. Samraj, Gun Ho Jang, Miki Haraguchi, Liang-I Kang, Marianna B. Ruzinova, Kiran R. Vij, Jacqueline L. Mudd, et al.comprised antibodies for FAP (Abcam Cat# ab53066, RRID:AB_880077), HMGA2 (Cell Signaling Technology Cat# 5269, RRID:AB_10694917), CK17 (Abcam Cat# ab109725, RRID:AB_10889888), CD45 (Abcam Cat# ab40763, RRID:AB_726545),

(6) A novel regulatory axis of MSI2-AGO2/miR-30a-3p-CGRRF1 drives cancer chemoresistance by upregulating the KRAS/ERK pathwayNeoplasiaJanuary 1, 2025Runhui Lu, Yafan Zhang, Ran Chen, Lian Li, Caihu Huang, Zihan Zhou, Yingting Cao, Hongyan Li, Junya Li, Yixin Zhang, et al.from Santa Cruz Biotechnology. Antibodies against AGO2 (#2897), Myc-tag (#2276), GFP (#2956), HMGA2 (#5269), EGFR (#2646S), ERK1/2(#9102S), p-ERK1/2(#4370S) and Normal Rabbit IgG (#2729) were from Cell Signaling Technology

(7) TGF-β and RAS jointly unmask primed enhancers to drive metastasisCellOctober 31, 2024Jun Ho Lee, Francisco J. Sánchez-Rivera, Lan He, Harihar Basnet, Fei Xavier Chen, Elena Spina, Liangji Li, Carles Torner, Jason E. Chan, Dig Vijay Kumar Yarlagadda, et al.(Cell Signaling Technology, Cat #: 3457S, 1:1,000), anti-HMGA2 antibody (Cell Signaling Technology, Cat #: 5269S, 1:1,000), anti-TGFBR2 antibody (Proteintech, Cat #: 66636-1-Ig, 1:1,000), anti-SNAIL antibody (Cell Signaling Technology

(8) Characterization of HMGA2 variants expands the spectrum of Silver-Russell syndromeJCI InsightMarch 22, 2024Avinaash V. Maharaj, Emily Cottrell, Thatchawan Thanasupawat, Sjoerd D. Joustra, Barbara Triggs-Raine, Masanobu Fujimoto, Sarina G. Kant, Danielle van der Kaay, Agnes Clement-de Boers, Alice S. Brooks, et al.monoclonal anti-FLAG M2 (Sigma-Aldrich, catalog F3165) and anti-HMGA2 (Cell Signaling Technology, catalog 5269) reconstituted in dilution buffer (1× PBS, 1% BSA, 0.3% Triton X-100) was added to cells and left at

(9) Adult progenitor rejuvenation with embryonic factorsCell proliferationMay 1, 2023Wang P, Liu X, Chen Y, Jun-Hao ET, Yao Z, Min-Wen JC, Yan-Jiang BC, Ma S, Ma W, Luo L, et al.0.5 μg/mL), Twist2 (ab66031; Abcam; 1 μg/mL), IMP1/2/3 (sc‐271785; Santa Cruz; 1:1000), HMGA2 (no. 5269S; Cell Signalling; 1:1000), tubulin (ab210797; Abcam; 1:1000), p53 (sc‐126; Santa Cruz; 1:100), citrate

(10) HIV Promotes Atherosclerosis via Circulating Extracellular Vesicle MicroRNAsInternational Journal of Molecular SciencesApril 20, 2023Andrea Da Fonseca Ferreira, Jianqin Wei, Lukun Zhang, Conrad J. Macon, Bernard Degnan, Dushyantha Jayaweera, Joshua M. Hare, Michael A. Kolber, Michael Bellio, Aisha Khan, et al.subsequently exposed to primary antibodies (primary antibodies used: Hmga2 Rabbit Ab, Cell Signaling #5269S; GAPDH Rabbit Ab, Cell Signaling #2118S, ApoA1, Thermo Fisher Scientific, 701239; ApoB, Thermo Fisher Scientific

(11) CRISPR/Cas9-3NLS/sgHMGA2@PDA nanosystem is the potential efficient gene editing therapy for gastric cancer with HMGA2 high expressionFrontiers in OncologySeptember 2, 2022Zhouying Wu et al.For immunofluorescence (IF) staining, sections were stained with anti-HMGA2 (1:300 dilution) (CST, #5269S), then revealed by Goat anti-Rabbit secondary antibodies labeled with Alexa Fluor 488 (Invitrogen).

(12) Hyaluronic acid restored protein permeability across injured human lung microvascular endothelial cellsFASEB bioAdvancesSeptember 1, 2022Sugita S, Naito Y, Zhou L, He H, Hao Q, Sakamoto A, Lee JW.Cell Signaling Technology), rabbit anti‐human VE‐cadherin (#2158, Cell Signaling Technology), rabbit anti‐human HMGA2 (#5269, Cell Signaling Technology), mouse anti‐human pAKT (#MAB887, R&D Systems), mouse anti‐human AKT (# MAB2055,

(13) Hmga2 protein loss alters nuclear envelope and 3D chromatin structureBMC biologyAugust 2, 2022Divisato G, Chiariello AM, Esposito A, Zoppoli P, Zambelli F, Elia MA, Pesole G, Incarnato D, Passaro F, Piscitelli S, et al.loaded in each lane. For western blotting experiments, the following antibodies were used: anti-Hmga2 #5269 (Cell Signaling) anti-H3K27me3 #3915S, anti-H3 (clone 0301) #39064, anti-H3K4me3 #61379, anti-H3K9me2

(14) Vestigial-like family member 3 stimulates cell motility by inducing high-mobility group AT-hook 2 expression in cancer cellsJournal of cellular and molecular medicineMay 1, 2022Hori N, Takakura Y, Sugino A, Iwasawa S, Nomizo K, Yamaguchi N, Takano H, Yamaguchi N.Smad2/3 (#8685), phospho‐Smad2 (#3108), phospho‐Smad3 (#9520), Snail (#3879), Slug (#9585), HMGA2 (#5269) from Cell Signaling Technology (Beverly, MA); TEAD4 (ab58310) from Abcam (Cambridge, UK); GFP (mFX75)

(15) CDK13-Mediated Cell Cycle Disorder Promotes Tumorigenesis of High HMGA2 Expression Gastric CancerFrontiers in Molecular BiosciencesAugust 26, 2021Zhouying Wu et al.(GE Healthcare Life Scientific, Amersham Imager 600). The primary antibodies are as follows: anti-HMGA2 (CST, #5269S), anti-CDK13 (Invitrogen, VB2774502), and anti-GAPDH (Santa Cruz Biotechnology, sc-32233). Cell Proliferation

(16) Selinexor versus doxorubicin in dedifferentiated liposarcoma PDXs: evidence of greater activity and apoptotic response dependent on p53 nuclear accumulation and survivin down-regulationJournal of experimental & clinical cancer research : CRMarch 1, 2021Zuco V, Pasquali S, Tortoreto M, Brich S, Percio S, Dagrada GP, Colombo C, Sanfilippo R, Lauricella C, Gounder M, et al.incubated with primary monoclonal antibodies: anti-MDM2 (#86,934, Cell Signaling Technology), anti-HMGA2 (#5269, Cell Signaling Technology), anti-cleaved CCP32 (#9661, Cell Signaling Technology), anti-STAT3 (#4904;

(17) The Nuclear Function of IL-33 in Desensitization to DNA Damaging Agent and Change of Glioma Nuclear StructureFrontiers in cellular neuroscienceJanuary 1, 2021Chung YH, Qian Q, Huang HY, Chiu WT, Yang CS, Tzeng SF.(WB) Rabbit anti HMGA1,Cell signaling Cat# 7777,1:2500 (WB) Rabbit anti HMGA1,Cell signaling Cat# 5269,1:2500 (WB) Biotinylated anti-mouse IgG,Abcam RRID: AB_954888,1:200 (IF) Biotinylated anti-rabbit

(18) miR-4651 inhibits cell proliferation of gingival mesenchymal stem cells by inhibiting HMGA2 under nifedipine treatmentInternational journal of oral scienceMarch 31, 2020Han X, Yang R, Yang H, Cao Y, Han N, Zhang C, Shi R, Zhang Z, Fan Z.performed according to a previous protocol.54 In this study, the primary antibodies against HMGA2 (Cat No. 5269, Cell Signaling Technology, USA), Cyclin E (Cat No. 05-363, Merck Millipore, Darmstadt, Germany), CDK2

(19) Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topologyMolecular OncologyOctober 1, 2019Syed Moiz Ahmed, Peter DrögeFisher Scientific, Waltham, MA, USA). Membranes were incubated with primary antibodies [α‐HMGA2 (CST 5269; 1 : 1000; RRID:AB_10694917), α‐TOP2A (TopoGEN TG2011‐1; 1 : 2000; RRID:AB_1934304), α‐TOP2B (Ab109524;

(20) Comparative Assessment of Antitumor Effects and Autophagy Induction as a Resistance Mechanism by Cytotoxics and EZH2 Inhibition in INI1-Negative Epithelioid Sarcoma Patient-Derived XenograftCancersJuly 19, 2019Stacchiotti S, Zuco V, Tortoreto M, Cominetti D, Frezza AM, Percio S, Indio V, Barisella M, Monti V, Brich S, et al.Cell Signaling), anti-EGR1 (#4153, Cell Signaling), anti-LC3B (#2775 Cell Signaling), anti-HMGA2 (#5269, Cell Signaling), anti-cleaved CCP32 (#9661, Cell Signaling), anti-cleaved PARP (#9541, Cell Signaling),

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HMGA2 Antibody

HMGA2 Antibody from Cell Signaling Technology

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Cell Signaling Technology

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HMGA2 Antibody from Cell Signaling Technology

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