PPARgamma (81B8) Rabbit mAb 2443S from Cell Signaling Technology

Product Specs
ItemPPARgamma (81B8) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number2443S
Quantity100 µl
ApplicationsW, IP, IF-IC, ChIP, ChIP-seq
ReactivityH, M
TargetPPAR-gamma
HostRabbit
NCBI Gene AliasesCIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma
ClonalityMonoclonal
NCBI Full Gene Nameperoxisome proliferator activated receptor gamma
Research AreaNuclear Receptor Signaling
Clone81B8
Molecular Weight53, 57

Questions and Answers

What applications has the PPARγ (81B8) Rabbit mAb been used for in scientific research?

The PPARγ (81B8) Rabbit mAb has been shown to work in various applications including Western Blotting, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, and ChIP-seq.

Can this antibody be used to study the role of PPARγ in adipogenesis?

Yes, the PPARγ (81B8) Rabbit mAb has been utilized in studies investigating adipogenesis. For instance, it was used to assess the expression of PPARγ in differentiating 3T3-L1 preadipocytes, highlighting its role in adipocyte differentiation and metabolism.

Is there evidence supporting the use of this antibody in cancer research?

Yes, the PPARγ (81B8) Rabbit mAb has been referenced in cancer research, particularly in studies examining its role in non-small cell lung cancer. Research indicates that PPARγ downregulation is associated with chemoresistance in these cancer cells, suggesting its importance in tumor biology.

What species does this antibody react with, and how has it been validated in studies?

The PPARγ (81B8) Rabbit mAb is reactive with both human and mouse proteins. It has been validated in studies where it was used to detect PPARγ levels in various cell types, including human neuroblastoma cells and mouse fibroblasts, demonstrating its utility across different biological contexts.

Cell Signaling Technology

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Danvers, Massachusetts 01923

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Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Arsenic-Induced PPARγ, with the Coordinated Action of p62, Inhibits Apoptosis and Necroptosis and Activates the DNA Damage Response in A549 Lung Cancer Cells, Leading to CarcinogenesisCellsApril 8, 2026Hak-Ryul Kim, Seon-Hee Oh(#26539), p21 (#73543), K63-polyubiquitin (#12930), RIP1 (#3493), phospho-γ-H2AX (S139, #2577), PPARγ (2443), and poly/mono-ADP-ribose (#89190) antibodies were purchased from Cell Signaling (Beverly, MA, USA).

(2) Metabolic activation of lumisterol to biologically active metabolites and their mechanism of actionBiochemical PharmacologyApril 1, 2026Andrzej T. Slominski, Tae-Kang Kim, Zorica Janjetovic, Senthilkumar Ravichandran, Yuwei Song, Ewa Podgorska, Radomir M. Slominski, Franz Ewendt, Yuhua Song, Justyna Szpotan, et al.sc-133088, Santa Cruz Biotechnology USA (dilution 1:50)) or ant-PPARγ antibody (PPARγ (81B8) rabbit mAb #2443 Cell Signaling Technology, USA (dilution 1:100)) in blocking solution overnight at 4°C. Next day, the

(3) B4GALT1 deficiency attenuates steatohepatitis by regulating the PPARγ/ACSL4 axisHepatol CommunApril 1, 2026Youjung Chien, Ruiqi Xia, Da Zhou, Yingjie Ai, Linfeng Wu, Xiaoqing Zeng, Shiyao Chenthen subjected to immunoprecipitation by overnight incubation at 4 °C with either anti-PPARγ antibody (2443; CST) or normal mouse IgG (B900620; Proteintech). Immune complexes were subsequently captured by incubation

(4) Three alternative promoters govern PGC-1α expression in chicken and are directly repressed by PPARγPoultry ScienceApril 1, 2026Jiaxin Huang, Fang Mu, Ming Lou, Fan Gao, Bolin Ning, Haoyu Luo, Xiaohong Yan, Yang Jing, Ning Wangwere then immunoprecipitated overnight at 4°C with an anti-PPARγ antibody (Cell Signaling Technology, #2443). Immune complexes were captured using ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology,

(5) A Standard Herbal Formula, CGAC, Attenuates Bone Loss by Normalizing Low-Bone Turnover Stagnation in an Orchiectomy-Induced Mouse ModelPharmaceuticals (Basel)March 31, 2026Dong-Cheol Baek, Min-Young Chae, Tae-Wook Woo, Chang-Gue Son, Eun-Jung Lee#9323S, Cell Signaling Technology), GSK3β (1:1000, #9315S, Cell Signaling Technology), PPAR-γ (1:1000, #2443S, Cell Signaling Technology), ALP (1:500, PA5-106391, Thermo Fisher Scientific), α-tubulin (1:1000, MA5-116869,

(6) Ovarian hormone deficiency enhances wood smoke-induced immune dysfunction via transcriptomic and metabolic alterationsToxicol SciMarch 25, 2026Mijung Oh, Sydnee Yazzie, Eunju Lim, Onamma Edeh, Charlotte McVeigh, Alicia Bolt, Jennifer M Gillette, Katherine E Zychowskiat 4 °C with primary antibodies against ARG1 (#93668, Cell Signaling Technology, 1:1,000), PPARγ (#2443, Cell Signaling Technology, 1:1,000), or Vinculin (#13901, Cell Signaling Technology, 1:1,000) diluted

(7) Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fateNucleic Acids ResMarch 19, 2026Patrizia Maria Christiane Nothnagel, Paul-Arthur Meslin, Jonas Aakre Wik, Damien Dufour, Yunna Erika Strøm, Magnar Bjørås, Jorrit Martijn Enserink, Bjørn Steen Skålhegg, Nolwenn Briand, Anthony Mathelier, et al.were carried out with antibodies for H3K27ac (ab4729, abcam), H3K27me3 (C15410195, Diagenode), PPARG (2443S, Cell Signaling and C15410367, Diagenode), and Dynabeads Protein A or G (10002D, 10004D, Thermo Fisher Scientific

(8) Colistin-resistance among Acinetobacter baumannii and Pseudomonas aeruginosa from clinical specimens in Africa: a systematic review and meta-analysisJAC Antimicrob ResistMarch 17, 2026Yalewayker Gashaw, Assefa Sisay, Ermias Getachew, Zelalem Asmare, Alene Geteneh, Ephrem Tamrat, Wagaw Abebe, Getachew Bitew, Abebe Birhanu, Mitkie Tigabie, et al.al.,46",2022,S. Africa,2033,1525,1958,75 "Anane et al.,47",2020,S. Africa,100,100,100,0 Total isolates,,,4249,2443,3952,297 Description of the studies included to assess colistin resistance in A. baumannii isolates

(9) Extensive enhancer crosstalk controls PPARG2 activation during adipogenesisNature CommunicationsMarch 15, 2026Anna Cetnarowska, Mette Hyldahl, Marcus Nygård, Hesam Dashti, Bo Vagner Hansen, Laura Kristine Holm, Kaja Madsen, Maria Stahl Madsen, Vallari Shukla, Esra Durmaz Mitchell, et al.L1293, Sigma-Aldrich), mouse-anti-FLAG (1:500, F1804-1MG, Sigma-Aldrich), rabbit-anti-PPARG (1:1000, 2443S, Cell Signaling) in PBS with 1.5% BSA, and then 1 hour at room temperature with secondary antibody:

(10) Cell type-specific epigenetic regulatory circuitry of coronary artery disease lociNat CommunMarch 10, 2026Dennis Hecker, Xiaoning Song, Nina Baumgarten, Anastasiia Diagel, Nikoletta Katsaouni, Ling Li, Shuangyue Li, Ranjan Kumar Maji, Fatemeh Behjati Ardakani, Lijiang Ma, et al.Cells were incubated overnight at 4 °C with rabbit anti-PPARgamma primary antibody (dilution 1:200, #2443S, Lot: 4, Cell Signaling Technology, Danvers, USA). After PBS washing, Donkey anti-rabbit lgG Highly

(11) Age-related adiponectin resistance in human skeletal muscle dysfunction: in vivo and in vitro evidenceJournal of Translational MedicineMarch 5, 2026Surina Surina, Lucia Scisciola, Manuela Giovanna Basilicata, Ada Pesapane, Rosaria Anna Fontanella, Nunzia Balzano, Alberta Maria Maddalena Palazzo, Asad Zia, Giovanni Tortorella, Zeeshan Ulfat, et al.overnight at 4 °C with primary antibodies, FAS (Cell signaling cat. #3180); PPAR-γ (Cell signaling cat. #2443); oxidative stress cocktail (Abcam, ab179843); SOD2 (Abcam, ab13533), AMPK (Abcam, ab32047), phospho-AMPK

(12) Upregulation of miR-146a-5p and miR-146b-5p limits IL-1β-mediated signaling in adipose tissue during polytrauma by downregulating IRAK1Front ImmunolMarch 4, 2026Antonia Mortsch, Julian Roos, Rebecca Halbgebauer, Ludmila Lupu, Annette Palmer, Anja Werberger, Ulrich Stifel, Martin Wabitsch, Markus Huber-Lang, Julia Zinngrebe, et al.rabbit, bioVendor, dilution 1:1,000), PLIN1 (Ab3526, polyclonal, rabbit, Abcam, dilution 1:1,000), PPARγ (2443, monoclonal, rabbit, CST, dilution 1:1,000), TRAF6 (PA5-29622, polyclonal, rabbit, Invitrogen, dilution

(13) Unraveling Endocannabinoid Signaling Pathways in Cisplatin‐Induced OtotoxicityThe FASEB JournalFebruary 28, 2026Sakthimala Palaniappan, Annamaria Tisi, Camilla Di Meo, Cristina Urbano, Georgina E. Fenton, Francesco Della Valle, Federico Fanti, Dario Compagnone, Marc Nazarè, Huib Versnel, et al.PA1‐823A,Rabbit,1:500,Nonfat dry milk 5%,Super Signal West Atto Ultimate Sensitivity PPARγ,Cell Signaling 2443,Rabbit,1:500,BSA 5%,Super Signal West Atto Ultimate Sensitivity NAPE‐PLD,Cayman Chemical 10305,Rabbit,1:200,Nonfat

(14) NAD+ sensing by PARP7 regulates the C/EBPβ-dependent transcription program during adipogenesisCell ReportsFebruary 24, 2026MiKayla S. Stokes, Yoon Jung Kim, Yonghyeon Kim, Sneh Koul, Shu-Ping Chiu, Chenqian Liu, Morgan Dasovich, Josue Zuniga, Tulip Nandu, Dan Huang, et al.(Thr235) (Cell Signaling Technology, 3084S; RRID:AB_2260359), PPARγ (81B8) (Cell Signaling Technology, 2443; RRID:AB_823598), cJun (Proteintech, 24909–1-AP; RRID:AB_2860574), cJun (Cell Signaling Technology,

(15) Postbiotic Metabolites of Proanthocyanidins Reduce Adipogenesis In Vitro by Suppressing De Novo LipogenesisMoleculesFebruary 17, 2026Wasitha P. D. W. Thilakarathna, Madumani Amararathna, H. P. Vasantha Rupasingheantibody (7074S), fatty acid synthase (FAS, 3180S), peroxisome proliferator-activated receptor-γ (PPAR-γ, 2443S), and β-actin (12620S) were purchased from Cell Signaling Technology, Inc., Danvers, MA, USA. 4.2.

(16) Quercus infectoria gall extract modulates adipocyte differentiation and lipid metabolism through dual regulation of adipogenesis and lipolysis in 3T3-L1 adipocytesAnim Cells Syst (Seoul)February 9, 2026Chanawee Jakkawanpitak, Suttiwan Wunnoo, Decha Sermwittayawong, Phanthipha Runsaeng, Thanaporn Amnuaikit, Supayang Piyawan Voravuthikunchaiincluded C/EBPβ (LAP) Antibody (#3087), C/EBPα (D56F10) XP® Rabbit mAb (#8178), PPARγ (81B8) Rabbit mAb (#2443), Fatty Acid Synthase (FAS) (C20G5) Rabbit mAb (#3180), and Phospho-HSL (Ser660) Rabbit mAb (#45804),

(17) PIK3CA Mutations Downregulate PPT1 to Promote Adipogenesis by Suppressing P300 Depalmitoylation and Phase SeparationAdvanced ScienceJanuary 29, 2026Hongrui Chen, Zening Huang, Rui Chang, Wei Gao, Yajing Qiu, Bin Sun, Chen Hua, Xiaoxi Lin24909‐1‐AP), and anti‐phospho‐JUN (Proteintech, 28891‐1‐AP), anti‐PPAR γ (Cell Signaling Technology, 2443S), anti‐C/EBP α (Proteintech, 13274‐1‐AP), anti‐FABP 4 (Proteintech, 12802‐1‐AP), anti‐P300 (Proteintech,

(18) Nutritional Modulation of Melatonin‐SIRT1 Signaling by Octanoic Acid‐Rich Enteral Nutrition Protects Against Radiation‐Induced Intestinal InjuryFood Science & NutritionJanuary 27, 2026Chenxi Zhou, Xiaohua Li, Chungen Xing, Chun Caonon‐fat milk and then incubated with primary antibodies against PPARγ (Cell Signaling Technology, CST #2443, 1:1000), SIRT1 (CST #9475, 1:1000), PGC‐1α (CST #2178, 1:1000), B‐cell lymphoma‐extra large (Bcl‐xL;

(19) Ovarian hormone deficiency enhances wood smoke-induced immune dysfunction via transcriptomic and metabolic alterationsbioRxivJanuary 20, 2026Mijung Oh, Sydnee Yazzie, Eunju Lim, Onamma Edeh, Charlotte McVeigh, Alicia Bolt, Jennifer M. Gillette, Katherine E. Zychowskiovernight at 4°C with primary antibodies against ARG1 (#93668, Cell Signaling Technology, 1:1000), PPARγ (#2443, Cell Signaling Technology, 1:1000), or Vinculin (#13901, Cell Signaling Technology, 1:1000) diluted

(20) Alfalfa Polysaccharide Improves Rabbit Growth by Modulating Gut Microbiota and Suppressing Inflammation Through PPARγ/NF-κB PathwayInternational Journal of Molecular SciencesJanuary 19, 2026Junying Xu, Fang Li, Yuanxin Zhu, Dongmei Liu, Wenjing Duan, Ran Yu, Boshuai Liu, Hao Sun, Zhichang Wang, Defeng Li, et al.above), NF-κB p65 (ZNGBIO, 823310, 1:1000), p-NF-κB p65 (Abmayt, TP56372F, 1:2000), and PPARγ (CST, 2443S, 1:1000). The secondary antibody for both were HRP-Goat Anti-Rabbit (Scracare, 5220-0336, 1:50,000)

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PPARgamma (81B8) Rabbit mAb

PPARgamma (81B8) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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PPARgamma (81B8) Rabbit mAb from Cell Signaling Technology

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