Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor ® 488 Conjugate) 4854S from Cell Signaling Technology

Product Specs
ItemPhospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor ® 488 Conjugate)
CompanyCell Signaling Technology
Price $477.00 Inquire
Catalog Number4854S
Quantity100 µl
ApplicationsFC-FP
ReactivityH, M, R, Mk
TargetS6 Ribosomal Protein (Ser235/Ser236) phosphate
ModificationsS6 Ribosomal Protein (Ser235/Ser236) phosphate
HostRabbit
NCBI Gene AliasesS6, eS6
Conjugate/TagAlexa Fluor ® 488
ClonalityMonoclonal
NCBI Full Gene Nameribosomal protein S6
Research AreaTranslational Control
IsotypeIgG
Clone2F9

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(1) Comparative study of rebaudioside A and rebaudioside Aα: physicochemical properties and in vivo metabolismnpj Science of FoodNovember 14, 2025Hye-Jin Kim, Hye-Jin Kang, Sangyi Lim, Ok Cheol Kim, Ki-Nam Yoon, Junhyeong Lee, Merc Emil Matienzo, Min-Jung Park, Bu-Soo Park, Jeong-Yong Cho, et al.shock protein 90 (HSP90; 4874) from Cell Signaling Technology; and phosphorylated perilipin (p-PLIN; #4854) from Vala Sciences. Immunoreactive bands were visualized using a luminescent image analyzer (ImageQuant™ LAS 4000 mini

(2) Imbalanced TGFβ signalling and autophagy drive erythroid priming of hematopoietic stem cells in β-thalassemiaNat CommunJuly 1, 2025Maria Rosa Lidonnici, Giulia Chianella, Nicole Mende, Hugo P. Bastos, Matteo Barcella, Ivan Merelli, Mariangela Storto, Valentina Romeo, Francesca Tiboni, Samantha Scaramuzza, et al.overnight at 4 °C with the following primary antibody: rabbit anti-phospho-S6 A488 (Cell Signaling, Cat. No 4854). Data were collected using FACSAria or FACS Canto using DIVA software (Becton Dickinson). Cell analyses

(3) Growth of human regulatory CD4+ T cells is more tightly controlled than effector T cells due to distinctive molecular programmingiScienceJune 20, 2025Alejandro Moro, Aixin Yu, Luis Nivelo, Zhen Gao, Yuguang Ban, Alejandro V. Villarino, Thomas R. Malek612598; RRID: AB_399881 Alexa Fluor 488 phosphoS6 (Ser235/236),Cell Signaling Technology,2F9; Cat# 4854S; RRID: AB_390782 Purified anti-human CD3 Antibody,Biolegend,OKT3; Cat#317301; AB_571926 Purified anti-human

(4) Azithromycin targets the CD27 pathway to modulate CD27hi T-lymphocyte expansion and type-1 effector phenotypeFrontiers in immunologyJanuary 1, 2024Ansari AW, Jayakumar MN, Ahmad F, Venkatachalam T, Salameh L, Unnikannan H, Raheed T, Mohammed AK, Mahboub B, Al-Ramadi BK, et al.Thermo Fisher Scientific) and stained with anti-pS6RP-Alexa Fluor 488 (Ser235/236, clone 2F9, Cat# 4854, Cell Signaling Technologies). Stained cells were acquired by a flow cytometer. 2.8 Statistical analysis For

(5) The Hippo pathway links adipocyte plasticity to adipose tissue fibrosisNature communicationsOctober 13, 2022Shen H, Huang X, Zhao Y, Wu D, Xue K, Yao J, Wang Y, Tang N, Qiu Y.89) (Cell Signaling, 59971, 1:1000), HSL (Cell Signaling, 4107, 1:1000), Perilipin 1 (Vala Sciences, 4854, 1:10,000), SMAD2/3 (Cell Signaling, 8685, 1:1000), p-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (Cell Signaling,

(6) Inhibition of autophagy with chloroquine dysregulates mitochondrial quality control and energetics in adipocytesArchives of pharmacal researchOctober 1, 2022Javaid HMA, Lim H, Shin S, Huh JY.h. Primary antibodies LC3I/II (#12741; Cell Signaling Technology, Danvers, MA, USA) and perilipin1 (#4854; Vala Sciences, San Diego, CA, USA) were incubated at room temperature for 2 h. Then, secondary antibodies

(7) Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neuronsBrain, behavior, & immunity - healthMay 1, 2022LaFever BJ, Kawasawa YI, Ito A, Imamura F.#644830, 1:300), rabbit anti-Phospho-S6 Ribosomal Protein (PS6; Cell Signaling Technology, catalog #4854S, 1:1000), rabbit anti-Parvalbumin (Millipore Sigma, catalog #MAB1572, 1:300), and rabbit anti-Somatostatin

(8) Hif-1a suppresses ROS-induced proliferation of cardiac fibroblasts following myocardial infarctionCell stem cellFebruary 3, 2022Janbandhu V, Tallapragada V, Patrick R, Li Y, Abeygunawardena D, Humphreys DT, Martin EMMA, Ward AO, Contreras O, Farbehi N, et al.RRID:AB_312798 Phospho-S6 Ribosomal Protein (Ser235/236) (2F9)-Alexa Fluor 488,Cell Signaling Technology,Cat# 4854; RRID: AB_390782 Anti-Col1a1 (E8I9Z),Cell Signaling Technology,Cat# 91144; RRID:AB_2800169 Anti-Perilipin-1,Abcam,Cat#

(9) Metabolic Defects of Peripheral T Cells in COVID-19 PatientsJournal of immunology (Baltimore, Md. : 1950)June 15, 2021Liu X, Zhao J, Wang H, Wang W, Su X, Liao X, Zhang S, Sun J, Zhang Z.washed and resuspended in 50 ml of anti-human phospho-S6 ribosomal protein (Ser235/236, catalog no. 4854S; Cell Signaling Technology) diluted 1:50 for 1 h at room temperature in the dark. The cells were then

(10) Encoding innately recognized odors via a generalized population codeCurrent BiologyMay 1, 2021Qiang Qiu, Yunming Wu, Limei Ma, C. Ron Yu,Thermo Fisher Scientific,Cat# R37118; RRID: AB_2556546 Rabbit anti-Phospho-S6 (Ser235/236),Cell Signaling,Cat# 4854; RRID: AB_390782 DAPI,Thermo Fisher Scientific,Cat# D1306; RRID: AB_2629482 Virus Strains,Virus Strains,Virus

(11) TGFβ restricts expansion, survival, and function of T cells within the tuberculous granulomaCell host & microbeApril 14, 2021Gern BH, Adams KN, Plumlee CR, Stoltzfus CR, Shehata L, Moguche AO, Busman-Sahay K, Hansen SG, Axthelm MK, Picker LJ, et al.Fisher; clone XMG1.2,"Cat# 505813, RRID:AB_493312" Anti-mouse pS6 AF488,Cell Signaling; clone 2F9,"Cat# 4854, RRID:AB_390782" Anti-mouse CD4 APC,BioLegend; clone RM4-4,"Cat# 116013, RRID:AB_2563024" Anti-mouse

(12) Acquisition of innate odor preference depends on spontaneous and experiential activities during critical periodeLifeMarch 26, 2021Qiu Q, Wu Y, Ma L, Xu W, Hills M Jr, Ramalingam V, Yu CR.anti-TAAR4,Gilad Barnea Lab,N/A,(1:500) Antibodies,Rabbit anti-Phospho-S6 (Ser235/236),Cell Signaling,4854; RRID:AB_390782,(1:1000) Antibodies,Goat anti-Guinea Pig Alexa Fluor 488,Thermo Fisher Scientific,A-11073;

(13) CD4⁺ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium DevelopmentFrontiers in immunologyJanuary 1, 2018Cobbold SP, Adams E, Howie D, Waldmann H.Pharmingen 561266) Ch2,Fix/Perm,Rabbit mAb anti-phospho-S6-AlexaFluor488 (Cell Signaling Technology #4854) Ch2Ch2,Fix/PermFix/Perm,Rabbit mAb anti-pan AKT-AlexaFluor488 (Cell Signaling Technology #5084S)Rabbit

(14) Autophagy maintains the metabolism and function of young and old stem cellsNatureMarch 9, 2017Ho TT, Warr MR, Adelman ER, Lansinger OM, Flach J, Verovskaya EV, Figueroa ME, Passegué E.antibodies: rabbit anti-phospho-S6 (Cell Signaling, 2211) or anti-phospho-S6 A488 (Cell Signaling, 4854), and guinea pig anti-p62 (Progen, GP62-C). Cells were then washed in PermWash and incubated in PermWash

(15) Hypermaintenance and hypofunction of aged spermatogonia: insight from age-related increase of Plzf expressionOncotargetJune 30, 2015Ferder IC, Wang N.cells with Alexa fluor 488-conjugated p-RPS6 (Ser235/236, clone 2F9, Cell Signaling Technology Catalog #4854) for 30 min. Cells were washed with PBS 3 times again and subject to FACS analysis. Extrinsic regulators

(16) Distinct Roles of the Phosphatidate Phosphatases Lipin 1 and 2 during Adipogenesis and Lipid Droplet Biogenesis in 3T3-L1 Cells*Journal of Biological ChemistryNovember 29, 2013Hiroshi Sembongi, Merce Miranda, Gil-Soo Han, Stylianos Fakas, Neil Grimsey, Joan Vendrell, George M. Carman, Symeon Siniossoglouand 2 (24), FABP4 (sc-18661; Santa Cruz Biotechnology), PPARγ (2443; Cell Signaling), perilipin 1 (4854; VALA Sciences), Fsp27 (26), actin (A2066; Sigma), and HA (sc-805; Santa Cruz Biotechnology). HRP-conjugated

(17) Multistage T cell-dendritic cell interactions control optimal CD4 T cell activation through the ADAP-SKAP55-signaling moduleJournal of immunology (Baltimore, Md. : 1950)September 1, 2013Mitchell JS, Burbach BJ, Srivastava R, Fife BT, Shimizu Y.phosphorylated c-Jun (Santa Cruz, #SC-822) and phosphorylated ribosomal S6 protein (Cell Signaling, #4854). All samples were acquired on a LSR II flow cytometer (BD Biosciences) and analyzed using Flowjo software

(18) FOXO3A directs a protective autophagy program in haematopoietic stem cellsNatureFebruary 21, 2013Warr MR, Binnewies M, Flach J, Reynaud D, Garg T, Malhotra R, Debnath J, Passegué E.antibodies: rabbit anti-phospho-mTOR (Cell Signaling, 5536), rabbit anti-phospho-S6-A488 (Cell Signaling, 4854), guinea pig anti-p62 (Progen, GP62-C), rabbit IgG control isotype (Cell Signaling, 3900S) or rabbit

(19) A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 PhosphorylationMethods in Molecular BiologyDecember 11, 2012Angela Papageorgiou, Joseph Avruchantibody should be aliquoted and stored at –80°C. Avoid freeze–thaw of the aliquots. 4000 is a clone 2F9 4854 but produced recombinantly. Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Cell Signaling Technology

(20) Self-antigen recognition by follicular lymphoma B-cell receptorsBloodNovember 15, 2012Kacey L. Sachen, Michael J. Strohman, Jonathan Singletary, Ash A. Alizadeh, Nicole H. Kattah, Chen Lossos, Elizabeth D. Mellins, Shoshana Levy, Ronald Levyfor CD3 and CD20 (BD Biosciences, 558021), and phosphorylated S6 ribosomal protein (Cell Signaling, 4854S). Cells were collected on an LSR II 3-laser cytometer (BD Biosciences), and data were analyzed with

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7/23/2021Great Phospho-S6 Ribosomal Antibody Xie ZhangWe wanted to examine Phospho-S6 Ribosomal expression in the healthy vs diseased state, so we used this antibody to identify .

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor ® 488 Conjugate)

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor ® 488 Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor ® 488 Conjugate) from Cell Signaling Technology

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