Fig 2: Expression of marker molecules of lymphocyte subpopulations in mouse spleen. Single-cell suspensions of spleen were prepared, and CD3+, CD4+, CD8+, CD19+, and CD20+ lymphocytes were detected using a flow cytometer. (A) Detection of CD3+ lymphocytes. (B) Detection of CD4+ and CD8+ lymphocytes. (C) Detection of CD19+ and CD20+ lymphocytes. N: normal control group. IM: immunosuppressive model group. Rl: R. mucilaginosa ZTHY2 with low concentration. Rm: R. mucilaginosa ZTHY2 with medium concentration. Rh: R. mucilaginosa ZTHY2 with high concentration. PC: positive control.

Fig 3: R. mucilaginosa ZTHY2 increased the levels of CDs in CTX-induced immunosuppressed mice. The CD4, CD8, CD19, and CD20 in the serum were detected by the ELISA. (A) The level of CD4. (B) The level of CD8. (C) The level of CD19. (D) The level of CD20. Comparison with N group, differences are indicated by “*”, ** (p < 0.01); comparison with IM group, differences are indicated by “#”, # (p < 0.05), ## (p < 0.01).

Fig 4: Expression of marker molecules of lymphocyte subpopulations in mice thymus. Single-cell suspensions of thymus were prepared, and CD3+, CD4+, CD8+, CD19+, and CD20+ lymphocytes were detected using a flow cytometer. (A) Detection of CD3+ lymphocytes. (B) Detection of CD4+ and CD8+ lymphocytes. (C) Detection of CD19+ and CD20+ lymphocytes. N: normal control group. IM: immunosuppressive model group. Rl: R. mucilaginosa ZTHY2 with low concentration. Rm: R. mucilaginosa ZTHY2 with medium concentration. Rh: R. mucilaginosa ZTHY2 with high concentration. PC: positive control.