Fig 1: CCR5 played a role in the recruitment of blood monocytes into the liver. RNA-seq analysis showed that the CCR5 gene is downregulated in KCs isolated from mice injected with FCM. (A) Volcano plot depicts -log10 Benjamini–Hochberg-corrected p-value versus log2 fold regulation for each gene for KCs sorted from FCM and saline (control)-treated mice after 2.5 h, (B) CCL3 levels increase and (C) CCL5 levels show a trend of increasing 5 h post-IV iron administration in liver lysate. (D,E) CCR5 expression on circulating blood monocytes post-FCM treatment by flow cytometry. The number of mice is n = 3 for RNA-seq experiments and two independent experiments of n = 5 for chemokine measurements. The data are expressed as Mean ± SEM. Statistical significance was calculated using ANOVA, followed by Bonferroni’s multiple comparison test as a post hoc test. where the mean of each group is compared to the control group (0 h). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.