CD44 Antibody (8E2F3)

Product Specs
ItemCD44 Antibody (8E2F3) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberNBP1-47386
ImmunogenPurified recombinant fragment of human CD44 (628-699) expressed in E. coli. [Uniprot: P16070]
TargetCD44
Conjugate/TagUnconjugated
Quantity0.1 ml (also 0.025 ml)
ApplicationsIF/IHC,Flow Cytometry,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,SDS-Page,Flow (Intracellular), Immunocytochemistry/ Immunofluorescence
IsotypeIgG1
ReactivityCanine,Human,Mouse, Rat
FormatPBS
ClonalityMonoclonal
Clone8E2F3
HostMouse
NCBI Full Gene NameCD44 molecule (Indian blood group)
Concentration1.0 mg/ml
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
ValidationKnockout Validated
Antibody TypePrimary Antibodies
Research AreaCancer, Cell Biology, Extracellular Matrix, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Signal Transduction, Stem Cell Markers
Molecular Weight82 kDa
PurityAmmonium sulfate precipitation
NCBI Gene AliasesMIC4, MDU2, HUTCH-1, IN, CSPG8, H-CAM, MC56, Hermes-1, HUTCH-I, MDU3, LHR, ECM-III, ECMR-III, HCELL, Pgp1, CDW44

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 ml (also 0.025 ml)

Questions and Answers

Can this CD44 antibody be used for studying cancer stem cells in colon cancer models?

Yes, the CD44 antibody (8E2F3) has been utilized in studies investigating cancer stem cell markers in colon cancer. For instance, in a study by Higuchi et al. (2013), the antibody was employed to analyze the expression of CD44 in drug-resistant LoVo human colon carcinoma cells, contributing to the understanding of cancer-initiating cells in this context.

Is this antibody effective for use in immunocytochemistry?

The CD44 antibody has been shown to work effectively in immunocytochemistry applications. In research conducted by Li et al. (2020), the antibody was used to stain RWPE1 cells, demonstrating its utility in visualizing CD44 expression in cultured cell lines.

What species can this antibody be used with, and does it have any relevance in stem cell research?

This antibody is reactive with canine, mouse, human, and rat species. It is particularly relevant in stem cell research as CD44 is a known marker for various stem cell populations, including mesenchymal and cancer stem cells, making it a valuable tool for studying stem cell biology across these species.

Are there any specific applications where this antibody has been cited in the literature?

The CD44 antibody has been cited in multiple applications, including Western blotting, ELISA, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, and immunoprecipitation. Its versatility in these applications has been documented in at least 15 scientific articles, highlighting its broad utility in various experimental setups.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Clinical OCT computation-modeled prognosis of coronary plaque progression with validation by fusogenic macrophage nano-targetingNano ConvergJuly 30, 2026Sewoom Baek, Hyun-Su Ha, Seyong Chung, Ji Youn Lee, Hyeongyun Choi, Chansik Kim, Seok Joon Lee, Seul-Gee Lee, Yong-Joon Lee, Jung-Sun Kim, et al.resuspended in 100 uL PBS with 0.5% FBS and incubated with primary antibodies against CD44 (1:100, NBP1-47386, Novus Biologicals, Centennial, CO, USA), E-cadherin (1:100, 3195, Cell Signaling, Danvers, MA, USA),

(2) Sulfotransferase signaling sustains fibroblast identity and antagonizes therapeutic cardiac reprogrammingNature CommunicationsJuly 17, 2026Michaela R. Romero, Sean Murphy, Yu-Ling Chang, Aashna Lamba, Sara Ancel, Alicia Llorente, James Marchant, Chun-Teng Huang, Caroline Kumsta, Peter Andersen, et al.Membranes were incubated overnight at 4 °C with primary antibodies against CD44 (Novus Biologicals, #NBP1-47386, 1:1000) and GAPDH (Cell Signaling, # D4C6R, 1:1000). Secondary antibodies (1:10,000) were applied for

(3) Loss of TC-PTP in keratinocytes leads to increased UVB-induced autophagyCell Death DiscoveryFebruary 28, 2025Obed Asare, Lindsey Shim, Cheol-Jung Lee, Jose Delgado, Natasha Quailes, Klarissa Zavala, Junsoo Park, Bilal Bin Hafeez, Yong-Yeon Cho, Subhash C. Chauhan, et al.(#2772); anti-cleaved caspase-3 (#9664); anti-PARP (#9542) from Cell Signaling Technology; anti-CD44 (#NBP1-47386) from Novus Biologicals and anti-β-Actin (sc-47778) from Santa Cruz Biotechnology. Analysis of UVB-induced

(4) Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cellsDrug Design, Development and TherapyJuly 3, 2013Hsin-chung Higuchi, Qing-Dong Henry Hsin-chung Lee, Wan-Chun Qing-Dong Ling, Chunh-Ming Wan-Chun Yu, Ta-Chun Ta-Chun Kao, Yi-Wei Yi-Wei Huang, Akon Higuchicatalog # NB100-92076; Novus Biologicals, Littleton, CO, USA), CD44 (mouse anti-human CD44, catalog # NBP1-47386; Novus), CD133 (rabbit anti-human CD133, catalog # PAB12663; Abnova, Taipei City, Taiwan), CD166 (rabbit

(5) "Plexin-B3 expression stimulates MET signaling, breast cancer stem cell specification, and lung metastasis"Cell reportsJanuary 1, 0001"Zuo Q, Yang Y, Lyu Y, Yang C, Chen C, Salman S, Huang TY, Wicks EE, Jackson W 3rd, Datan E, et al."

(6) The human placenta shapes the phenotype of decidual macrophagesCell reportsJanuary 1, 0001"Vondra S, Höbler AL, Lackner AI, Raffetseder J, Mihalic ZN, Vogel A, Saleh L, Kunihs V, Haslinger P, Wahrmann M, et al."

(7) Steroids and β2-agonists regulate hyaluronan metabolism in asthmatic airway smooth muscle cellsAmerican journal of respiratory cell and molecular biologyJanuary 1, 0001"Papakonstantinou E, Klagas I, Karakiulakis G, Hostettler K, S'ng CT, Kotoula V, Savic S, Tamm M, Roth M."

(8) TLR2 enhances ovarian cancer stem cell self-renewal and promotes tumor repair and recurrence"Cell cycle (Georgetown, Tex.)"January 1, 0001"Chefetz I, Alvero AB, Holmberg JC, Lebowitz N, Craveiro V, Yang-Hartwich Y, Yin G, Squillace L, Gurrea Soteras M, Aldo P, et al."

(9) Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applicationsCell and tissue bankingJanuary 1, 0001"Hassanzadeh H, Matin MM, Naderi-Meshkin H, Bidkhori HR, Mirahmadi M, Raeesolmohaddeseen M, Sanjar-Moussavi N, Bahrami AR."

(10) Smooth Muscle Cell Reprogramming in Aortic AneurysmsCell stem cellJanuary 1, 0001"Chen PY, Qin L, Li G, Malagon-Lopez J, Wang Z, Bergaya S, Gujja S, Caulk AW, Murtada SI, Zhang X, et al."(clone M-20),Santa Cruz,Cat#sc-1506; RRID:AB_2161037 Mouse monoclonal anti-CD44,NOVUS BIOLOGICALS,"Cat#NBP1-47386, RRID:AB_10010339" Mouse monoclonal anti-CD68 (clone KP1),abcam,"Cat#ab955, RRID:AB_307338" Mouse

(11) Breast osteoblast-like cells: a new biomarker for the management of breast cancerBritish journal of cancerJanuary 1, 0001"Scimeca M, Urbano N, Bonfiglio R, Schillaci O, Bonanno E."

(12) Chemotherapy induces enrichment of CD47⁺/CD73⁺/PDL1⁺ immune evasive triple-negative breast cancer cellsProceedings of the National Academy of Sciences of the United States of AmericaJanuary 1, 0001"Samanta D, Park Y, Ni X, Li H, Zahnow CA, Gabrielson E, Pan F, Semenza GL."

(13) Novel K6-K14 keratin fusion enhances cancer stemness and aggressiveness in oral squamous cell carcinomaOncogeneJanuary 1, 0001"Tsai FJ, Lai MT, Cheng J, Chao SC, Korla PK, Chen HJ, Lin CM, Tsai MH, Hua CH, Jan CI, et al."

(14) Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNK signalingCell death & diseaseJanuary 1, 0001"Baek M, Kim M, Lim JS, Morales LD, Hernandez J, Mummidi S, Williams-Blangero S, Jang IS, Tsin AT, Kim DJ."(sc-47778) from Santa Cruz Biotechnology; anti-FLAG® M2 (#F3165) from Sigma-Aldrich; and anti-CD44 (#NBP1-47386) from Novus Biologicals.

(15) Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYCScientific reportsJanuary 1, 0001"Li H, Chaitankar V, Zhu J, Chin K, Liu W, Pirooznia M, Rodgers GP."Cell Signaling Technology Inc., #14369); anti-CK13 (clone EPR3671, Abcam, Cat# ab92551); anti-CD44 (NBP1-47386, 8E2F3, Novus Biologicals); chicken anti-CK5 (Biolegend, Cat#905901); anti-SCGB1A1 (clone 394324, Novus Biologicals

(16) Mapping and modeling human colorectal carcinoma interactions with the tumor microenvironmentNature communicationsJanuary 1, 0001"Li N, Zhu Q, Tian Y, Ahn KJ, Wang X, Cramer Z, Jou J, Folkert IW, Yu P, Adams-Tzivelekidis S, et al."°C, then washed incubated overnight at 4 °C with primary antibodies against CD44 [Novus Biologicals, NBP1-47386, 1:400] and SPP1 [Rockland, 600-401-ET6, 1:200]. After washing, the slides were incubated with PLA probes

(17) HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapyThe Journal of clinical investigationJanuary 1, 0001"Salman S, Meyers DJ, Wicks EE, Lee SN, Datan E, Thomas AM, Anders NM, Hwang Y, Lyu Y, Yang Y, et al."

(18) Tumor-derived macrophage migration inhibitory factor promotes an autocrine loop that enhances renal cell carcinomaOncogeneJanuary 1, 0001"Du W, Wright BM, Li X, Finke J, Rini BI, Zhou M, He H, Lal P, Welford SM."or CD44 knockdown cells probed with CD74 (NB100-78089, Novus Biologicals, Littleton, CO, USA), CD44 (NBP1-47386, Novus Biologicals), p27 and b-actin antibodies. (e) Western of RCC4 cells with indicated knockdown

(19) Impairing proliferation of glioblastoma multiforme with CD44+ selective conjugated polymer nanoparticlesScientific reportsJanuary 1, 0001"Lubanska D, Alrashed S, Mason GT, Nadeem F, Awada A, DiPasquale M, Sorge A, Malik A, Kojic M, Soliman MAR, et al."

(20) Association between inflammation and cancer stem cell phenotype in breast cancerOncology lettersJanuary 1, 0001"Jeong YJ, Oh HK, Park SH, Bong JG."SC‑7034; clone C‑20; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), CD44 (dilution, 1:1,000; cat. no. NBP1‑47386; clone, 8E2F3; Novus Biologicals, LLC, Littleton, CO, USA), CD4 (ready‑to‑use dilution; cat. no. PA0368;

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