MOUSE ANTI RAT IgD:Biotin

Published customer image:
Biotinylated Mouse anti Rat IgD antibody, clone MARD-3 (MCA190B) used for the evaluation of IgD expression on rat splenocytes by flow cytometry.
Image caption:
Three-colour cytometry was used to analyze FO-B cells and MZ-B cells.
A single neonatal rat splenic cell suspension was stained with FITC conjugated anti-rat IgM (HIS40), biotinylated anti-rat IgD (MaRD3; AbD Serotec, Oxford, UK), and APC anti-rat CD90/Thy1.1 (HIS51). Biotinylated mAb were revealed with streptavidin conjugated to the tandem fluorochrome PE-Cy5.5. Lymphocytes were sequentially gated by forward scatter and side scatter. Acquisition gates were set to exclude the unwanted immature B cells (CD90⁺-APC). Gate settings were set appropriately for FO-B cells (CD90^negIgD^highIgM^low) and MZ-B (CD90^negIgM^highIgD^low) cells.

From: Hendricks J, Visser A, Dammers PM, Burgerhof JGM, Bos NA, Kroese FGM (2019)
The formation of mutated IgM memory B cells in rat splenic marginal zones is an antigen dependent process.

This image is from an open access article distributed under the terms of the.

Published customer image:
Biotinylated Mouse anti Rat IgD antibody, clone MARD-3 (MCA190B) used for the evaluation of IgD expression on rat splenocytes by flow cytometry.
Image caption:
Three-colour cytometry was used to analyze FO-B cells and MZ-B cells.
A single neonatal rat splenic cell suspension was stained with FITC conjugated anti-rat IgM (HIS40), biotinylated anti-rat IgD (MaRD3; AbD Serotec, Oxford, UK), and APC anti-rat CD90/Thy1.1 (HIS51). Biotinylated mAb were revealed with streptavidin conjugated to the tandem fluorochrome PE-Cy5.5. Lymphocytes were sequentially gated by forward scatter and side scatter. Acquisition gates were set to exclude the unwanted immature B cells (CD90⁺-APC). Gate settings were set appropriately for FO-B cells (CD90^negIgD^highIgM^low) and MZ-B (CD90^negIgM^highIgD^low) cells.

From: Hendricks J, Visser A, Dammers PM, Burgerhof JGM, Bos NA, Kroese FGM (2019)
The formation of mutated IgM memory B cells in rat splenic marginal zones is an antigen dependent process.
PLoS ONE 14(9): e0220933.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Published customer image:
Biotinylated Mouse anti Rat IgD antibody, clone MARD-3 (MCA190B) used for the evaluation of IgD expression on rat splenocytes by flow cytometry.
Image caption:
Three-colour cytometry was used to analyze FO-B cells and MZ-B cells.
A single neonatal rat splenic cell suspension was stained with FITC conjugated anti-rat IgM (HIS40), biotinylated anti-rat IgD (MaRD3; AbD Serotec, Oxford, UK), and APC anti-rat CD90/Thy1.1 (HIS51). Biotinylated mAb were revealed with streptavidin conjugated to the tandem fluorochrome PE-Cy5.5. Lymphocytes were sequentially gated by forward scatter and side scatter. Acquisition gates were set to exclude the unwanted immature B cells (CD90⁺-APC). Gate settings were set appropriately for FO-B cells (CD90^negIgD^highIgM^low) and MZ-B (CD90^negIgM^highIgD^low) cells.

From: Hendricks J, Visser A, Dammers PM, Burgerhof JGM, Bos NA, Kroese FGM (2019)
The formation of mutated IgM memory B cells in rat splenic marginal zones is an antigen dependent process.
PLoS ONE 14(9): e0220933.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.