Fig 1: TNFα does not significantly affect proliferation and extracellular molecule expression in OA chondrocytes. (A) CD34, CD44, CD59, CD74, CD90, CD105, CD146 and CD164 surface markers of chondrocytes were evaluated by flow cytometry. (B) Chondrocytes were exposed to TNFα and assessed by water-soluble tetrazolium salt assay. n=5, one-way ANOVA with Tukey's post hoc test. (C) Chondrocytes were exposed to 25 ng/ml TNFα for 24 h and assessed by flow cytometry. IgG was used as a control. OA, osteoarthritis; TNFα, tumor necrosis factor α; ACAN, aggrecan; SOX9, SRY-box transcription factor 9.