Fig 1: ILC3s are enriched in lung-draining mediastinal lymph nodes (MedLNs) and have high expression of MHC class II(A and B) Representative plots and quantitative graphs of frequencies (A) or total numbers of ILC2s and ILC3s (B) in mouse lung parenchyma and MedLNs (pooled from 2 independent assays, n = 8–10). Cells are gated on live CD45+ Lin− CD127+ (lineage markers: CD3e, CD5, CD8a, NK1.1, CD11c, CD11b, B220, and F4/80) as in Figure S1A.(C) Representative histograms of specific markers on gated ILC2s and ILC3s in mouse MedLNs (representative of 6 mice).(D) Representative plots and quantitative graphs of ratios of ILC2s and ILC3s in human lung and MedLN tissues. Cells were gated on live CD45+ Lin− (CD3, CD4, CD5, CD11b, CD11c, CD14, CD19, FcεRI) CD127+ populations from matched lung and MedLN tissues pooled from 5 donors.(E) Representative histograms of specific markers on ILC2s and ILC3s from human MedLN tissues (representative from 5 donors). Data are represented as mean ± SEM. Statistics are calculated by Student’s t test. See also Figure S1.