Progranulin/PGRN Antibody AF2420 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemProgranulin/PGRN Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2420
NCBI Gene AliasesPEPI, CLN11, GEP, PGRN, PCDGF, GP88
ApplicationsBioassay,FRET,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry, ELISA Capture
Conjugate/TagUnconjugated
TargetProgranulin/PGRN
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse
IsotypeIgG
Quantity100 ug (also 25 ug)
HostGoat
NCBI Full Gene Namegranulin precursor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human Progranulin/PGRN, Thr18-Leu593, Accession # P28799
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Progranulin/PGRN in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunohistochemistry in human brain tissue?

Yes, the Progranulin/PGRN antibody has been successfully used for immunohistochemistry in human brain tissue, as demonstrated in studies examining its expression in various neurodegenerative conditions, including Alzheimer's disease and frontotemporal dementia (, ).

What applications has this antibody been validated for in scientific research?

The Progranulin/PGRN antibody has been shown to work in several applications, including Western blotting, immunocytochemistry, immunofluorescence, and immunohistochemistry, as well as immunoprecipitation and flow cytometry (, ).

Is this antibody effective in detecting progranulin in mouse models?

Yes, the Progranulin/PGRN antibody has been utilized in mouse models to study the effects of progranulin deficiency and its role in neurodegenerative diseases, including its protective effects against excitotoxicity in neuronal cultures (, ).

What is the significance of using this antibody in studies related to neurodegenerative diseases?

The Progranulin/PGRN antibody is significant in neurodegenerative disease research as it helps elucidate the role of progranulin in lysosomal function and neuroprotection, particularly in conditions like frontotemporal dementia and Alzheimer's disease, where progranulin levels are critical for neuronal health (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Mucin-binding protein shuttles enable delivery of brain-targeted therapeuticsbioRxivApril 12, 2026Sophia M. Shi, Gabrielle S. Tender, Jian Xiong, Josephine K. Buff, Hannah I. Park, Justin H. Mendiola, Edward N. Wilson, Monther Abu-Remaileh, Carolyn R. Bertozzi, Tony Wyss-CorayPA1-9000), rat anti-LAMP1 (1:100, Thermo, 14-1071-82), and goat anti-progranulin (1:100, Biotechne, AF2420). Cells were subsequently washed three times with PBS, stained with the appropriate Alexa Fluor-conjugated

(2) Granulin loss and TMEM106B risk converge on lysosomal C-terminal fragment pathology in frontotemporal dementiabioRxivMarch 31, 2026Yi Zeng, Jian Xiong, Anastasiia Lovchykova, Thao Phuong Nguyen, Abigail Song, Semma W. Gitler, Monther Abu-Remaileh, Aaron D. Gitler9091P or 15665S), Mouse LAMP1 antibody (1:1000, DSHB, 1D4B), progranulin antibody (1:1000, R&D Systems, AF2420), CTS B (1:1000, Cell Signaling Technology, 31718T), PDI antibody (1:1000, Enzo Life Sciences, ADI-SPA-891-D),

(3) Systematic characterization of existing and novel inducible transgenic systems in human pluripotent stem cells after prolonged differentiationbioRxivJanuary 16, 2026Michael D. Gallagher, Andrew S. Khalil, Qi Liu, Tenzin Lungjangwa, Antar Drews, Blake F. Hanan, Moritz List, Henry Hardart, David J. Mooney, Rudolf Jaenischantibodies and dilutions included: abcam ab125247 anti-GAPDH (1:10,000), abcam ab6556 anti-GFP (1:5000), R&D AF2420 anti-GRN (1.25ug/mL), Novus Biologicals NBP2-61812 anti-KLF2 (1:365), Santa Cruz Biotechnology sc-17785

(4) Analysing the effect of full-length and C-terminally truncated progranulin on proliferation, colony formation, and migration in HepG2 and U87 cellsScientific reportsNovember 28, 2025Hofer AM, Tobler L, Ruepp MD, Mühlemann O.Antibodies,Primary Antibodies,Primary Antibodies,Primary Antibodies Goat anti-PGRN,1:250–500,Polyclonal,AF2420,R&D Systems Mouse anti-PGRN,1:100,Monoclonal,"sc-377,036",Santa Cruz Mouse anti-Flag,1:1000,Monoclonal,F1804,Sigma-Aldrich Mouse

(5) Reduction of sphingomyelinase activity associated with progranulin deficiency and frontotemporal dementiaNeurobiology of DiseaseSeptember 1, 2025Nicholas R. Boyle, Stephanie N. Fox, Aniketh S. Tadepalli, Nicholas T. Seyfried, Thomas Kukar, Eliana M. Ramos, Alissa L. Nana, Salvatore Spina, Lea T. Grinberg, Bruce L. Miller, et al.probed overnight with primary antibodies to human progranulin (0.04 μg/mL, goat polyclonal, R&D systems #AF2420, RRID: AB_2114489) and DYKDDDDK (FLAG) tag (1:25000, rabbit monoclonal, D6W5B, Cell Signaling Technology,

(6) Granulins rescue inflammation, lysosome dysfunction, lipofuscin, and neuropathology in a mouse model of progranulin deficiencyCell reportsDecember 24, 2024Root J, Mendsaikhan A, Taylor G, Merino P, Nandy S, Wang M, Araujo LT, Ryu D, Holler C, Thompson BM, et al.RESOURCE,SOURCE,IDENTIFIER ,, Antibodies,, ,, Goat polyclonal anti-human progranulin,R and D Systems,Cat# AF2420; RRID: AB_2114489 Rabbit polyclonal anti-human granulin 2,This paper,N/A Rabbit polyclonal anti-human

(7) Progranulin AAV gene therapy for frontotemporal dementia: translational studies and phase 1/2 trial interim resultsNature MedicineMay 14, 2024Jeffrey Sevigny, Olga Uspenskaya, Laura Dean Heckman, Li Chin Wong, Daniel A. Hatch, Ambika Tewari, Rik Vandenberghe, David J. Irwin, Dario Saracino, Isabelle Le Ber, et al.tissue lysate supernatants using human progranulin capture antibody mix for plate coating (R&D Systems, AF2420, at 1:100), and mouse anti-human progranulin detection antibody (R&D Systems, MAB2420, at 1:1,000).

(8) Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidaseNature communicationsFebruary 16, 2024Takahashi H, Bhagwagar S, Nies SH, Ye H, Han X, Chiasseu MT, Wang G, Mackenzie IR, Strittmatter SM.

(9) Interleukin-6 Induces Stem Cell Propagation through Liaison with the Sortilin-Progranulin Axis in Breast CancerCancersDecember 8, 2023Berger K, Persson E, Gregersson P, Ruiz-Martínez S, Jonasson E, Ståhlberg A, Rhost S, Landberg G.loading control. The nitrocellulose membrane was probed with a primary anti-progranulin (0.75 μg/mL, AF2420, R&D Systems), anti-IL-8 (1:1000, ab18672, Abcam, Cambridge, UK), anti-IL-6 (1:1000, ab9324, Abcam)

(10) Altered plasma protein profiles in genetic FTD - a GENFI studyMolecular neurodegenerationNovember 15, 2023Ullgren A, Öijerstedt L, Olofsson J, Bergström S, Remnestål J, van Swieten JC, Jiskoot LC, Seelaar H, Borroni B, Sanchez-Valle R, et al.(M067-3 from MBL International; MA1-70053, PA5-34943, 34–1000 from Invitrogen Antibodies; MAB2037-SP, AF2420, AF3154 from R&D Systems). The mean coefficient of variance per 384-well plate (n = 3) was less than

(11) Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodentsNature communicationsSeptember 19, 2023He Y, Kaya I, Shariatgorji R, Lundkvist J, Wahlberg LU, Nilsson A, Mamula D, Kehr J, Zareba-Paslawska J, Biverstål H, et al.

(12) AAV-GRN partially corrects motor deficits and ALS/FTLD-related pathology in Tmem106b(-/-)Grn(-/-) miceiScienceJuly 21, 2023Feng T, Minevich G, Liu P, Qin HX, Wozniak G, Pham J, Pham K, Korgaonkar A, Kurnellas M, Defranoux NA, et al.AB_2107436 goat anti-CathD,R&D Systems,Cat# AF1029; RRID: AB_2087094 goat anti-human PGRN,R&D Systems,Cat# AF2420; RRID: AB_2114489 sheep anti-mouse PGRN,R&D Systems,Cat# AF2557; RRID: AB_2114504 mouse anti-CHAT,R&D Systems

(13) Multi-modal Proteomic Characterization of Lysosomal Function and Proteostasis in Progranulin-Deficient NeuronsbioRxiv : the preprint server for biologyFebruary 24, 2023Hasan S, Fernandopulle MS, Humble SW, Frankenfield AM, Li H, Prestil R, Johnson KR, Ryan BJ, Wade-Martins R, Ward ME, et al.,Jackson ImmunoResearch,#016-620-084,Immunofluorescence,1:1000 Streptavidin-488,Jackson ImmunoResearch,#016-540-084,Immunofluorescence,1:1000 PGRN,R&D systems,#AF2420,Immunofluorescence,1:1000 Rhodamine Red Donkey anti-Mouse IgG (H+L),Jackson ImmunoResearch,#715-295-151,Immunofluorescence,1:1000 Rhodamine

(14) Pathological 25 kDa C-Terminal Fragments of TDP-43 Are Present in Lymphoblastoid Cell Lines and Extracellular Vesicles from Patients Affected by Frontotemporal Lobar Degeneration and Neuronal Ceroidolipofuscinosis Carrying a GRN MutationInternational journal of molecular sciencesNovember 9, 2022Cimini S, Bellini S, Saraceno C, Benussi L, Ghidoni R, Giliani SC, Puoti G, Canafoglia L, Giaccone G, Rossi G.USA). The membranes were incubated overnight at 4 °C with the following primary antibodies: anti-hPGRN (AF2420, 1:500, R&D systems, Minneapolis, MN, USA); anti-TSG101 (ab83, 1:500, Abcam, Cambridge, UK); anti-Alix

(15) Deficiency of the frontotemporal dementia gene GRN results in gangliosidosisNature communicationsOctober 7, 2022Boland S, Swarup S, Ambaw YA, Malia PC, Richards RC, Fischer AW, Singh S, Aggarwal G, Spina S, Nana AL, et al.GM2 monoclonal antibody (clone MK1-16) (TCI America, Cat#A2576), human progranulin (R&D systems, Cat#AF2420), anti-mouse progranulin polyclonal antibody that recognizes an epitope at amino acids 198–21430, PCNA

(16) Efficient progranulin exit from the ER requires its interaction with prosaposin, a Surf4 cargoThe Journal of cell biologyFebruary 7, 2022Devireddy S, Ferguson SM.anti-LAMP1 (H4A3; DSHB), rabbit anti-saposin C (clone H-81, SC-32587; Santa Cruz), goat anti-progranulin (AF2420; R&D Systems), rabbit anti-calnexin (C5C9; Cell Signaling Technology), mouse anti-GM130 (610822; BD

(17) Outcomes of progranulin gene therapy in the retina are dependent on time and route of deliveryMolecular therapy. Methods & clinical developmentSeptember 10, 2021Zin EA, Han D, Tran J, Morisson-Welch N, Visel M, Kuronen M, Flannery JG.Antibody,Company,Dilution Sheep anti-mPGRN,"R&D Systems (Minneapolis, MN, USA), AF2557",1:500 Goat anti-hPGRN,"R&D Systems, AF2420",1:500 Rabbit anti-IBA1,"Fujifilm Wako Diagnostics (Mountain View, CA, USA), 019-19741","1:1,000" Rabbit

(18) Delivering progranulin to neuronal lysosomes protects against excitotoxicityThe Journal of biological chemistrySeptember 1, 2021Davis SE, Roth JR, Aljabi Q, Hakim AR, Savell KE, Day JJ, Arrant AE.in PBS. Antibodies The following antibodies were used for Western blot: Progranulin (R&D Systems #AF2420, 1:500 and MilliporeSigma #HPA008763, 1:500), NeuN (MilliporeSigma #MAB377, 1:500), GFAP (Agilent #Z033429,

(19) Regulated control of gene therapies by drug-induced splicingNatureAugust 1, 2021Monteys AM, Hundley AA, Ranum PT, Tecedor L, Muehlmatt A, Lim E, Lukashev D, Sivasankaran R, Davidson BL.aliquoted and frozen at −80 °C for future use as needed. Human progranulin antibodies (R&D Systems AF2420 and R&D Systems MAG2420) were labelled with donor Tb-fluorophore and acceptor d2-fluorophore, respectively

(20) Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin miceActa neuropathologica communicationsApril 1, 2021Frew J, Nygaard HB.anti-Iba1 (WAKO, 019-19741, 1:500), anti-Lamp1 (BD Biosciences, 553792, 1:500), anti-Pgrn (R&D Systems, AF2420, 1:1000), TDP-43 (Proteintech, 10782-2-AP, 1:500), anti-Tuj1 (Neuromics, CH23005, 1:500), anti-Vgat

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