Fig 1: Insulin signaling through the Akt/TSC2 pathway is maintained in the livers of rats fed an HFD compared with a CON diet. Representative blots showing phosphorylation of Akt on Ser473 (A) and TSC2 on Ser939 (B), assessed by Western blot analysis. (C) Blood glucose concentrations were assessed before or after 60 min of refeeding. Values are means ± SEMs;n = 9–10 (A, B) or 17–18 (C). Labeled means without a common letter differ,P < 0.05.†Different from CON, P = 0.07. Akt, a kinase also referred to as protein kinase B; CON, control; HFD, high-fat diet; M.E., main effect; -P, phosphorylated; S473, Ser473; S939, Ser939; -T, total; TSC2, tuberous sclerosis complex 2.