Prox1 Antibody AF2727 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemProx1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF2727
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Immunocytochemistry/ Immunofluorescence
IsotypeIgG
Quantity100 ug (also 25 ug)
TargetProx1
HostGoat
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityAvian - Chicken,Human,Mouse,Porcine,Primate - Callithrix jacchus (Common Marmoset),Rat,Transgenic Mouse,Xenograft,Fish - Danio rerio (Zebrafish)
NCBI Full Gene Nameprospero homeobox 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Prox1, Pro2-Gln259, Accession # Q92786
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Prox1 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

What applications has the Prox1 antibody been used for in published studies?

The Prox1 antibody has been utilized in various applications including immunofluorescence, immunohistochemistry, and Western blotting. For instance, it has been shown to work effectively in immunofluorescence for tissue sections and in Western blotting to detect human Prox1 protein levels in different experimental contexts, as noted in multiple studies.

Can this antibody be used to study Prox1 in mouse models?

Yes, the Prox1 antibody has been successfully used in mouse models. For example, in a study examining the role of Prox1 in the developing human retina, the antibody was employed for immunostaining in mouse brain sections, indicating its applicability in mouse tissues.

Is the Prox1 antibody effective for flow cytometry applications?

Yes, the Prox1 antibody has been cited for use in flow cytometry. In one study, it was used to analyze cell populations, demonstrating its utility in characterizing Prox1 expression in various cell types.

What species can the Prox1 antibody detect, and how has this been validated?

The Prox1 antibody is reactive with multiple species, including human, mouse, rat, chicken, and zebrafish. Its specificity has been validated in studies where it successfully detected Prox1 in different species, confirming its broad applicability in comparative studies of Prox1 function across species.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Sox5 controls cell cycle progression in intermediate progenitor cells during dentate gyrus developmentFront Cell Dev BiolJuly 23, 2026Paula Tirado-Melendro, Lingling Li, Cristina Medina-Menéndez, Pilar Jurado-Angulo, Pilar Rodríguez-Martín, Laura García-Redondo, Katarzyna Bilińska, Aixa V. MoralesNES8967981, Aves Labs), NeuN (1:500, MAB377, Merck Milipore), Prox1 (1:1000, MAB5654, Merck and 1:500, AF2727, R&D Systems), Sox2 (1:500, AF 2018, R&D Systems), Sox5 (1:500, A. Morales), Sox6 (1:1000, M. Wegner,

(2) Progressive intestinal tumor cell plasticity, Myc activation, and loss of Lgr5+ tumor stem cell lineage commitment upon Wnt depletionSci AdvJuly 10, 2026Marika Lassila, Fatemeh Seyednasrollah, Cinzia Bessone, Maritta Räisänen, Inkeri Vuori, Davide G. Berta, Johanna Aspholm, Tatiana V. Petrova, Lauri A. Aaltonen, Kari Alitalo, et al.RRID:AB_2199302), rabbit anti-Tcf1 (1:200; #MA5-14972, Thermo Fisher Scientific), goat anti-Prox1 (1:200; AF2727, R&D Systems, RRID:AB_2170716), rabbit anti-Lyz1 (1:500; Dako A0099, RRID:AB_2341230), goat anti-Msx1

(3) Kynurenic acid mediates epicardial fat-induced lymphatic metabolic dysfunction in atrial fibrillationNature CommunicationsMay 19, 2026Masaki Takahashi, Ichitaro Abe, Naofumi Yoshida, Daisuke Katoh, Daishi Fujita, Shunsuke Goto, Hirochika Yamasaki, Toshiyuki Ko, Yue Jiang, Yu He, et al.Catalog no. PA1-16635 Invitrogen, Carlsbad, California), goat anti PROX1 antibody (5 µg/mL Catalog no. AF2727 R&D Systems), rabbit anti TAGLN antibody (1:100 Catalog no. #40471 Cell Signaling Technology) and rat

(4) TNF-mediated hilar interneuron loss and aberrant granule cell migration are associated with chronic cognitive deficits following TBIBrain, behavior, and immunityMarch 1, 2026Harris EA, Budianto S, Arnab S, de Jager C, Hodes GE, Bethea JR, Theus MH.

(5) Single-Cell Profiling of the Developing Organ of Corti Identifies Etv4/5/1 as Key Regulators of Pillar Cell IdentitybioRxivJanuary 24, 2026Susumu Sakamoto, Matthew W. Kelleygoat anti-NGFR (R&D Systems AF367, RRID:AB_2152638 at 1:500 dilution), goat anti-PROX1 (R&D Systems AF2727, RRID:AB_2170716 at 1:500 dilution), goat anti-SOX2 (R&D Systems AF2018, RRID:AB_355110 at 1:500 dilution),

(6) Establishment of a human inner ear model reveals that gentamicin C2b is substantially less ototoxic than clinical gentamicinBiomedicine & PharmacotherapyJanuary 6, 2026Minjin Jeong, Hiroaki Mohri, Dongjun Han, Masaharu Sakagami, Konstantina M. Stankovicmonoclonal GATA3 (Cell Signaling Technology, 5852; 1:800), goat polyclonal IgG PROX1 (R&D systems, AF2727; 1:20), and mouse monoclonal cytochrome c (Cell Signaling Technology, 12963; 1:300). The next day, cells

(7) A Multipotent PROX1+ Tumor Stem/Progenitor Cell Population Emerges during Intestinal Tumorigenesis and Mediates Radioresistance in Colorectal CancerCancer researchDecember 1, 2025Kallio P, Bessone C, Seyednasrollah F, Brodkin J, Lassila M, Högström J, González-Loyola A, Petrova TV, Haglund C, Alitalo K.for experiments within 5 to 20 passages of thawing. Goat anti–human PROX1 antibodies (R&D Systems, #AF2727, RRID: AB_2170716) were used for immunoprecipitation and subsequent mass spectrometry analysis. Irradiation In vitro

(8) Inhibiting cholesterol synthesis halts rhabdomyosarcoma growth via ER stress and cell cycle arrestEMBO Molecular MedicineNovember 17, 2025Nebeyu Yosef Gizaw, Kalle Kolari, Pauliina Kallio, Kari Alitalo, Riikka Kivelä(Molecular Probes) at a dilution of 1:500. For patient-derived RMS samples, PROX1 (R and D Systems Cat# AF2727, RRID:AB_2170716) staining was carried out utilizing the BenchMark XT automated system on 5-μm-thick

(9) Human Lymphatic Endothelial Cells Expressing NRASQ61R Model Characteristics of Kaposiform LymphangiomatosisPediatric Blood & CancerNovember 1, 2025Dermot A. Fox, C. Griffin McDaniel, Patricia Pastura, Timothy D. Le Cras2 weeks after Dox was added to the media. Primary antibodies were against PROX1 (1:250; R&D Systems AF2727) and PDPN (1:250; Sigma-Aldrich P5374), and secondary antibodies were donkey anti-rabbit 488 (1:200;

(10) Acute and chronic nasal inflammation induces lymphangiogenesis in the olfactory mucosa in miceBrain Behav Immun HealthOctober 7, 2025Suzuho Komaki, Ryuichi Imai, Yuzuki Sugimoto, Rei Settsu, Aki Obara, Atsuyoshi Shimada, Robert Dantzer, Geoffroy Laumet, Fumiaki Imamura, Sanae Hasegawa-IshiiPF,No LYVE-1,goat,R&D Systems,1:500,AF2125,No VEGFR-3,goat,R&D Systems,1:500,AF743,No Prox-1,goat,R&D Systems,1:1000,AF2727,Yes (Tris-EDTA Buffer) Ki-67,rat,Invitrogen,1:500,14-5698-82,Yes (Tris-EDTA Buffer) F4/80,rat,Abcam,1:200,ab6640,No List

(11) PROX1 is necessary for cellular patterning, innervation and differentiation in the mouse organ of CortiDevelopmental BiologyOctober 1, 2025Shubham Kale, Dimitri Traenkner, Ellison J. Goodrich, Satish R. Ghimire, Thomas M. Coate, Michael R. Deanssc-7466), Rabbit anti-P75 (1:900, PFA, Millipore Sigma 07–476), Goat anti-PROX1 (1:300, PFA, R&D Systems AF2727), Rabbit anti-PROX1 (1:1000, PFA, Reliatech 102-PA32AG), Goat anti-SOX2 (1:200, PFA, Santa Cruz, 17320),

(12) Lymphatic endothelial mTORC1 instructs metabolic and developmental signaling during lymphangiogenesisDevelopmental CellSeptember 8, 2025Fei Han, Summer Simeroth, Jie Zhu, Irma Gryniuk, Atul Pranay, Weiqing Chen, Yuan Wang, Yuanyuan Cai, Zhiyuan Shen, Guangyu Wang, et al.(Cell Signaling Technology, 2867), GLS (Abcam, ab156876), MYC (Abcam, ab32072), PROX1 (R&D Systems, AF2727), phosphorylated S6RP (Ser240/244) (Cell Signaling Technology, 5364), total S6RP (Cell Signaling Technology,

(13) Interleukin-19 is a Novel Lymphangiocrine Factor Inducing Lymphangiogenesis and Lymphatic Junctional RegulationArteriosclerosis, Thrombosis, and Vascular BiologyJuly 1, 2025Amanda M. Peluzzo, Amanda St. St. Paul, Cali B. Corbett, Sheri E. Kelemen, Silvia Fossati, Xiaolei Liu, Michael V. Autieri(final concentration 1ug/mL, Invitrogen, Cat. PA5–85552; final concentration 0.2ug/mL, R&D Systems, Cat. AF2727), Angpt2 (final concentration 0.64ug/mL, Invitrogen, Cat. PA5–27297), C2CD4B (final concentration 0.5ug/mL,

(14) Interleukin-19 is a Novel Lymphangiocrine Factor Inducing Lymphangiogenesis and Lymphatic Junctional RegulationArterioscler Thromb Vasc BiolMay 15, 2025Amanda M. Peluzzo, Amanda St. Paul, Cali B. Corbett, Sheri E. Kelemen, Silvia Fossati, Xiaolei Liu, Michael V. Autieri(final concentration 1ug/mL, Invitrogen, Cat. PA5–85552; final concentration 0.2ug/mL, R&D Systems, Cat. AF2727), Angpt2 (final concentration 0.64ug/mL, Invitrogen, Cat. PA5–27297), C2CD4B (final concentration 0.5ug/mL,

(15) Embryological cellular origins and hypoxia-mediated mechanisms in PIK3CA-driven refractory vascular malformationsEMBO Molecular MedicineApril 16, 2025Sota Torii, Keiki Nagaharu, Nanako Nakanishi, Hidehito Usui, Yumiko Hori, Katsutoshi Hirose, Satoru Toyosawa, Eiichi Morii, Mitsunaga Narushima, Yoshiaki Kubota, et al.RRID:AB_2631039; M0823, DAKO, 1:100, RRID:AB_2114471), Prox1 (11-002, AngioBio, 1:100, RRID:AB_10013720; AF2727, R&D Systems, 1:100, RRID:AB_2170716), VEGFR3 (AF743, R&D Systems, 1:100, RRID:AB_355563), GFP (ab290,

(16) Epicardial VEGFC/D signaling is essential for coronary lymphangiogenesisEMBO ReportsMarch 24, 2025Ester de la Cruz, Vanessa Cadenas, Susana Temiño, Guillermo Oliver, Miguel TorreseBioscienceTM 50-5851-82, Rat 1:100/1:200; LYVE1 ReliaTech 03-PA50S, Rabbit 1:200; PROX1 R&D systems AF2727, Goat 1:200; SM22α abcam ab14106, Rabbit 1:200; VEGFD Cusabio CSB-PA07554A0Rb, Rabbit 1:200; VEGFR3

(17) Translatome analysis reveals cellular network in DLK-dependent hippocampal glutamatergic neuron degenerationeLifeMarch 11, 2025Ritchie EM, Acar D, Zhong S, Pu Q, Li Y, Zheng B, Jin Y.anti-Satb2,Abcam,Ab51502;RRID:AB_882455,IF (1:500) cells Antibody,Goat polyclonal anti-Prox1,R&D Systems,AF2727;RRID:AB_2170716,IF (4 µg/mL) Antibody,Rabbit polyclonal anti-Map3k13,Sigma-Aldrich,HPA016497;RRID:AB_10670027,IF

(18) A single-cell atlas of normal and KRASG12D-malformed lymphatic vesselsJCI insightJanuary 28, 2025Fernandes LM, Griswold-Wheeler D, Tresemer JD, Vallejo A, Vishlaghi N, Levi B, Shapiro A, Scallan JP, Dellinger MT.donkey serum for 30 minutes. Slides were then incubated overnight with goat anti-Prox1 (R&D Systems, AF2727; 1:250) and rabbit anti-Lyve1 (Abcam, ab14917; 1:250) primary antibodies diluted in TBST plus 5% BSA.

(19) Direct specification of lymphatic endothelium from mesenchymal progenitorsNat Cardiovasc ResJanuary 2, 2025Irina-Elena Lupu, David E. Grainger, Nils Kirschnick, Sarah Weischer, Erica Zhao, Ines Martinez-Corral, Hans Schoofs, Marie Vanhollebeke, Grace Jones, Jonathan Godwin, et al.(R&D Systems, AF3628, 1:250), PECAM1 (D. Vestweber, clones 5D2.6 and 1G5.1, 15 μg ml−1), PROX1 (R&D Systems, AF2727, 1:200), PROX1 (Proteintech, 11067-2-AP, 1:100), PROX1 (Reliatech, 102-PA32, 1:100), PROX1 (Abcam, ab225414,

(20) A novel AAV9-dual microRNA-vector targeting GRIK2 in the hippocampus as a treatment for mesial temporal lobe epilepsyMolecular Therapy - Methods & Clinical DevelopmentDecember 12, 2024Stéphane J. Baudouin, April R. Giles, Nick Pearson, Severine Deforges, Chenxia He, Céline Boileau, Nicolas Partouche, Andreas Borta, Justine Gautron, Morgane Wartel, et al.incubated with anti-GFP antibody (Abcam, AB-300798, 1:1000) and with anti-Prox1 antibody (R&D systems AF2727, 1:100) for 48 h at 4°C. Slices were then incubated for 2 h with Alexa Fluor conjugated secondary antibodies

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Prox1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Prox1 Antibody from R&D Systems, a Bio-Techne Brand

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