Chromatin Immunoprecipitation Hela (1.2*10⁶) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-TP53 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP07889HU) against the P21 promoter.

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Rat brain tissue, Mouse brain tissue
All lanes: TP53 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 44 kDa

Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

"Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Rat brain tissue, Mouse brain tissue
All lanes: TP53 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 44 kDa"