Western Blot analysis of SIRT4 in extracts of various cell lines using SIRT4 Polyclonal Antibody (Product # PA5-96727) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of SIRT4 in extracts of various cell lines. Samples were incubated with SIRT4 Polyclonal antibody (Product # PA5-96727) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.