IL-1 beta/IL-1F2 Antibody AF-401-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-1 beta/IL-1F2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF-401-NA
NCBI Gene AliasesIL1-BETA, IL-1, IL-1beta, IL1F2, Il-1b, IL1beta
ApplicationsBead-based Immunoassay,Bioassay,ELISA Standard,Western Blots,MUSTag PCR Assay,Western Blot,Flow Cytometry,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vitro assay,Proximity Ligation Assay,In vivo assay,Simple Western,Neutralization,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Cell Culture,ELISA Capture
HostGoat
IsotypeIgG
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Porcine,Pseudomonas aeruginosa,Rabbit,Rat,Transgenic Mouse
TargetIL-1 beta/IL-1F2
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
NCBI Full Gene Nameinterleukin 1 beta
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse IL-1ß/IL-1F2, Val118-Ser269, Accession # NP_032387
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse IL-1ß/IL-1F2 in direct ELISAs. In direct ELISAs, less than 15% cross-reactivity with recombinant porcine IL-1ß is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study IL-1β production in macrophages during inflammasome activation?

Yes, the IL-1 beta/IL-1F2 antibody has been utilized in various studies to detect IL-1β production in macrophages, particularly in the context of NLRP3 inflammasome activation. For instance, it was used in Western blot analyses to measure pro-IL-1β levels in macrophages responding to stimuli such as lipopolysaccharides and Pseudomonas aeruginosa infection (, ).

Is this antibody effective for use in flow cytometry applications?

The IL-1 beta/IL-1F2 antibody has been shown to work in flow cytometry applications, allowing for the analysis of IL-1β expression in various cell types, including neutrophils and macrophages (, ).

What species can this antibody detect, and is it suitable for cross-species studies?

This antibody is reactive with multiple species, including porcine, human, rabbit, rat, and mouse. This broad reactivity makes it suitable for cross-species studies, allowing researchers to investigate IL-1β in various animal models and human samples (, ).

Has this antibody been cited in studies related to inflammatory diseases?

Yes, the IL-1 beta/IL-1F2 antibody has been cited in numerous studies focusing on inflammatory diseases, including its role in conditions such as sepsis and chronic inflammation. For example, it was used to assess IL-1β levels in models of polymicrobial sepsis and in studies examining the effects of various treatments on inflammasome activation (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Protocol to study inflammasome activation in induced pluripotent stem cell-derived macrophagesSTAR ProtocJuly 28, 2026Chloe M. McKee, Melanie Cranston, Emma C. McKay, Mohammad Arefian, Ben C. Collins, Rebecca C. CollRRID:AB_2916166 Anti-GSDMD C-terminal (1:1000),Abcam,ab210070; RRID:AB_2893325 Anti-IL-1β (1:1000),R&D Systems,AF-401-NA; RRID:AB_416684 Anti-NLRP3 (Cryo-2) (1:1000),Adipogen,AG-20B-0014; RRID:AB_2490202 Anti-NLRP3 (D4D8T)

(2) DcR3 Suppresses Lipopolysaccharide-Induced Aggresome-like Structures in Macrophages via Inhibition of Reactive Oxygen Species and p38 MAPKInt J Mol SciJuly 20, 2026Chun-Hung Lee, Duen-Yi Huang, Shie-Liang Hsieh, Yuan-Shen Chen, Wan-Wan LinAntibody of NLRP3/NALP3 (AG-20B-0014) was from AdipoGen (San Diego, CA, USA). Antibody against IL-1β (AF-401-NA) was purchased from R&D Systems (Minneapolis, MN, USA). Antibody against iNOS (ab178945), K48 (ab140601),

(3) NLRP3 Inhibitor KBD3536 Attenuates Acute Inflammation, Radiation-Induced Skin Injury, and Early Metabolic Dysfunction in Preclinical ModelsPharmaceuticals (Basel)July 14, 2026Xinying Qian, Fei Ye, Zhiyong Li, Hongzhu Chu, Zeng Xu, Wenyuan Peng, Xueya Liang, Hongchuan Zhao, Yan Tang, Pan Zhong, et al.(PA5-79740; Thermo Fisher Scientific, Waltham, MA, USA) and goat anti-mouse IL-1β polyclonal antibody (AF-401-NA; R&D Systems, Minneapolis, MN, USA). Secondary antibodies included Leica ImmunoDetector Reagent (DS9800;

(4) Entadamide A-β-D-glucopyranoside attenuates psoriatic dermatitis by restoring keratinocyte homeostasis and suppressing neutrophil extracellular trapsJournal of ethnopharmacologyJune 28, 2026Zhang ZH, Wang XY, Quan SL, Zeng ZY, Zhang KJ, Wu YL, Zhan ZY, Nan JX, Lian LH.

(5) Threat assessment shapes neutrophil cell fate upon inflammasome activationSci AdvJune 26, 2026See Jie Yow, Bing-Chen Wu, Hai Shin Pung, Safwah Nasuha Rosli, Hui Wen Yeap, Ghin Ray Goh, Kay En Low, Isabelle Bonne, Jizhong Shi, Shuizhou Yu, et al.were against caspase-1 (AG-20B-0042-C100; 1:1000; Adipogen), GSDMD (ab209845; 1:1000; Abcam), IL-1β (AF-401-NA; 1:1000; R&D Systems), NLRP3 (Cryo-2; 1:1000; Adipogen), NLRC4 (ab201792, 1:1000; Abcam), Pyrin (ab214772;

(6) Soyeom-Jetong mixture attenuates NLRP3 inflammasome-mediated inflammationFrontiers in PharmacologyJune 16, 2026Qianying Ye, Sojung Lee, Yeon-Cheol Park, Myung-Seo Kim, Wonnam Kim, Jong-Sik Jin, Dong-Keun Kim, Seoyeon Jang, Sumin Lee, Nahyun Lee, et al.Antibodies against alpha-tubulin (sc-5286) was procured from Santa Cruz Biotechnology. Human IL-1β (AF-401-NA) and mouse IL-1β antibodies (AF-201-NA) were purchased from R&D Systems. LPS from Porphyromonas gingivalis

(7) Infection-induced glucose starvation triggers NINJ1-dependent macrophage lysis and Candida escapeNature CommunicationsJune 10, 2026Harshini Weerasinghe, Orawan Tulyaprawat, Helen Stölting, Johannes Sonnberger, Byron Mobbs, Joshua Nickson, Theresa Lange, Bryce van Denderen, Tricia L. Lo, Tim Bastian Schille, et al.rotating platform with the following primary antibodies (1:1000 dilution): anti-IL-1β (R&D Systems, #AF-401-NA), anti-caspase-1 (Adipogen, AG-20B-0042-C100), and anti-GSDMD (Abcam, ab209845). The next day, membranes

(8) Caspase-8 silences cell death-independent constitutive immune activation driven by tonic TNF-αEMBO ReportsJune 8, 2026Germana Lentini, Francesco Coppolino, Agata Famà, Giuseppe Valerio De Gaetano, Federica Grasso, Luigi Fiore, Alessia Berbiglia, Silvia Carnevale, Isabella Venza, Egil Lien, et al.beta Actin- Loading Control antibody,Abcam,Cat #ab8229 Mouse IL-1 beta /IL-1F2 Antibody,R&D Systems,Cat #AF-401-NA "anti-Caspase-1 (p20) (mouse), mAb (Casper-1)",AdipoGen Life Sciences,Cat #AG-20B-0042-C100100 "anti-Caspase-1

(9) Exercise preserves β-cell function in type 2 diabetes by reshaping intra-islet macrophage–β-cell crosstalkLife MetabolismJune 2, 2026Miqi Yang, Yanping Zhou, Qing-Qian Wu, Wenjing Zhang, Rui Zhang, Zhuoying Yang, Ting Yu, Ruo-Ran Wang, Hongxing Fu, Qi Fu, et al.mouse anti-Caspase-1 (p20) (dilution 1:1000; AG-20B-0042; AdipoGen); mouse anti-IL-1β (dilution 1:1000; AF- 401-NA; R&D Systems); and rabbit anti-HSP90 (dilution 1:1000; C45G5; Cell Signaling Technology). Secondary

(10) Loss of Proximal Tubule Lactate Dehydrogenase A (LDHA) Exacerbates Nephrotoxic Acute Kidney Injury Through Metabolic DysregulationAmerican Journal of Physiology-Renal PhysiologyJune 1, 2026Yan Lu, Anna A. Zmijewska, Yanfeng Zhang, Gunars Osis, Matthew D. Cheung, Landon Wilson, Yanlin Jiang, Sudeepthi Vejendla, Amie Traylor, Stephen Barnes, et al.Proteintech, cat. 19987–1-AP), Pfkp (1:1,000; Proteintech, cat. 13389–1-AP), IL-1β (1:1,000; R&D Systems, cat. AF-401-NA), IL-6 (1:1,000; Invitrogen, cat. AMC0862), and β-actin (1:10,000; Sigma, cat. A2228) as loading control.

(11) Muscone Promotes PINK1/Parkin-Associated Mitophagy to Suppress NLRP3 Inflammasome Activation: Implications for Endotoxemia TherapyPharmaceuticals (Basel)May 23, 2026Ziwei Yan, Minrui Li, Dan Li, Wentian Hua, Haoxue Cao, Yufei Li, Li Che, Xiyi Chen, Zhicheng Lai, Yi Wang, et al.(3033S), and LC3B (83506S) were purchased from Cell Signaling Technology. Anti-pro-IL-1β antibody (AF-401-NA) was purchased from R&D Systems (Minneapolis, MN, USA). Anti-COX IV (AF6549), anti-tubulin (AF5012),

(12) Discovery of a novel cannabidiol-derived transient receptor potential vanilloid 4 inhibitor to reduce pulmonary edema and lung vascular permeability in miceCellular & Molecular Biology LettersMay 17, 2026Yassir Arfath, Pankaj Singh Cham, Tusharika Kotra, Rahila Akhter, Sumeer Ahmed, Mandeep Kour, Sheikh Tasduq Abdullah, Parvinder Pal Singh, Sheikh Rayees, Zabeer AhmedR&D Systems, USA: mouse IL-6 ELISA kit, DY406; mouse IL-1β ELISA kit, DY401; and IL-1β primary antibody, AF-401NA. Cell Signaling Technology, USA: phospho-NF-κB p65, 3033S; anti-rabbit IgG, 7074P2; anti-mouse IgG,7076;

(13) Piperine, major ingredient of Piper nigrum, attenuates fibrogenesis and inflammation in hepatic fibrosis by switching STAT3 phosphorylation at Ser727Chinese Herbal MedicinesMay 10, 2026Xu Dai, Yunuo Wu, Lihua Lian, Zhenyu Cui, Jixing Nan, Yanling Wufrom Santa Cruz Biotechnology Inc. (Dallas, USA). Additionally, the primary antibody against IL-1β (AF-401-NA, lot number: NP3521052) was procured from R&D Systems (Minneapolis, USA). The fetal bovine serum (FBS,

(14) Cofilin-1 is a redox-sensitive guard of the NLRP3 inflammasomeNature immunologyMay 1, 2026Park YH, Batu ED, Matthiasardottir B, Kim Y, Akkaya-Ulum YZ, Kastner DL, Chae JJ.supernatants were analyzed by immunoblot with antibodies to human IL-1β (AF-201-NA, R&D Systems); mouse IL-1β (AF-401-NA, R&D Systems); cofilin-1 (sc-33779, rabbit polyclonal; sc-53934, mouse monoclonal, specific for reduced

(15) Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injuryJournal of Clinical InvestigationApril 15, 2026Miao Wang, Zhentai Huang, Zhihong Du, Jiajing Shan, Qing Ye, Lingxiao Lu, Ming Jiang, Fei Xu, Ziyang Liu, David J.R. Fulton, et al.(Cell Signaling Technology, 89332S), mouse anti-GAL3 (Santa Cruz Biotechnology, sc-32790), mouse anti–IL-1β (R&D Systems, AF-401-NA), rabbit anti–cathepsin B (Abcam, ab214428), and mouse anti–β-actin (R&D Systems, MAB8929). tMCAO. tMCAO

(16) Thymelaea hirsuta (L.) Endl. extract attenuates NLRP3 inflammasome activation via modulation of ATPase activityFront PharmacolApril 15, 2026Seongjong Lee, Seoyeon Jang, Hangyeol Lee, Ali Khorchani, Abdelhamid Khaldi, Mi Jin Lee, Dong Keun Yi, Soo-Yong Kim, Sangho Choi, Hyungseok Seo, et al.antibody was obtained from Cell Signaling Technology (United States). Antibodies specific for human IL-1β (AF-401-NA) and mouse IL-1β (AF-201-NA) were purchased from R&D Systems (United States). Highly cross-adsorbed

(17) Daucosterol alleviates osteoarthritis by targeting chondrocyte senescence via inhibiting of the JNK pathwayInternational immunopharmacologyApril 15, 2026Chen L, Li J, Wang S, Ren R, Xiong W, Gan X, Kang H, Wen Y, Li J, He L.

(18) METTL18 ensures pancreatic function by maintaining proper translation and proteostasisMolecular MetabolismApril 1, 2026Tadahiro Shimazu, Megumi Gowa, Ayane Kataoka, Takehiro Suzuki, Kotaro Tomuro, Yuichi Shichino, Naoshi Dohmae, Shintaro Iwasaki, Yoichi Shinkai(cat#ab48187), anti-phospho-EIF2A (cat#ab32157), and anti-EIF2A (cat#ab5369) (Abcam); anti-IL-1β (cat#AF-401-NA; R&D Systems). Unless otherwise indicated, all antibodies were used for Western blotting at a 1:1000

(19) Kidney and brain-expressed protein upregulation in the anterior cingulate cortex mediates chronic post-thoracotomy pain by the phospho-protein kinase Mζ/glutamate receptor 1 signaling pathway and neuroinflammation in male ratsPainApril 1, 2026Xin Zhou, Jia-Xin Chen, Jing-Jiao Shen, Ping-Ping Liu, Lu Chang, Chang-Yu Jiang, Jun Zhang, Ying Xiong, Le Wang, Bing-Bing Cao, et al.(bs-11570R; Bioss), GluR1 (ab174785; Abcam), p-PKMζ (sc174785; Santa Cruz), TNF-α (BS-1857; Bioworld), IL-1β (AF-401-NA; R&D Systems, Minneapolis, MN), neuron-specific nuclear protein (NeuN, MAB377; Millipore, Burlington,

(20) IL-24 regulates corneal inflammation and fibrosis in fungal keratitis via the caspase-11/gasdermin D pathwayThe ocular surfaceApril 1, 2026Yang H, Liu W, Zhang D, Xu Q, Lee J, Sun J, Xue S, Sun X, Che C.

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