IL-27 Antibody AF2526 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-27 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF2526
NCBI Gene AliasesIL35B, IL-27, IL27B, IL27A, IL30, IL-27B, IL-27A, p28, IL27p28
ApplicationsBioassay,ELISA Development,ELISA Development (Capture),Immunohistochemistry,Western Blot,Neutralization,ELISA Capture (Matched Antibody Pair)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman
IsotypeIgG
HostGoat
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
TargetIL-27
NCBI Full Gene NameEpstein-Barr virus induced 3, interleukin 27
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human IL-27 (R&D Systems, Catalog # 2526-IL), Arg21-Lys229 (IL-27 EBI-3 subunit), Phe29-Pro243 (IL-27 p28 subunit), Accession # Q14213 (EBI-3 subunit), AAM34498 (p28 subunit)
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IL-27 in ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

Add to Compare List

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) IL-27 shapes NK cell heterogeneity and function in colorectal cancerJ Immunother CancerJuly 29, 2026Lorenzo Lucantonio, Andrea Kosta, Ludovica Schiano, Francesca Sozio, Silvia Ruggeri, Giovanna Peruzzi, Giuseppe Pietropaolo, Arianna M Candelotti, Laura Beltrame, Mattia Laffranchi, et al.(anti-mouse IL-27 BD Biosciences, cat# 568409, clone MM27.7B1 or anti-human IL-27 p28 (R&D System, cat# AF2526)) antibody or of isotype control antibody was added to co-cultures and refreshed after 24 hours. Co-cultures

(2) Human interleukin-12α and EBI3 are cytokines with anti-inflammatory functionsScience advancesOctober 27, 2023Hildenbrand K, Bohnacker S, Menon PR, Kerle A, Prodjinotho UF, Hartung F, Strasser PC, Catici DAM, Rührnößl F, Haslbeck M, et al.

(3) Fungal sensing by dectin-1 directs the non-pathogenic polarization of T(H)17 cells through balanced type I IFN responses in human DCsNature immunologyDecember 1, 2022Gringhuis SI, Kaptein TM, Remmerswaal EBM, Drewniak A, Wevers BA, Theelen B, D'Haens GRAM, Boekhout T, Geijtenbeek TBH.IL-1β (AF-201-NA, R&D Systems), IL-23 (AF1716, R&D Systems), IL-12 (AF-219-NA, R&D Systems), IL-27 (AF2526, R&D Systems) or TGF-βRII (AF-241-NA, R&D Systems) or normal goat IgG (AB-108-C; R&D Systems) as a control.

(4) PD-L1⁺CD8⁺ T cells enrichment in lung cancer exerted regulatory function and tumor-promoting toleranceiScienceFebruary 18, 2022Zheng Y, Han L, Chen Z, Li Y, Zhou B, Hu R, Chen S, Xiao H, Ma Y, Xie G, et al.ab53097 anti-human Ki-67,abcam,Cat# ab15580 anti-human CD3,abcam,Cat# ab5690 Human IL-27 Antibody,R&D Systems,Cat# AF2526 ,, Biological samples,Biological samples,Biological samples ,, Human fresh lung tumor and adjacent

(5) T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancerOncoimmunologyJanuary 1, 2021O'Connor RA, Chauhan V, Mathieson L, Titmarsh H, Koppensteiner L, Young I, Tagliavini G, Dorward DA, Prost S, Dhaliwal K, et al.(BioLegend Cat. No. 501126) "IL-27 Goat anti-Human, Polyclonal, R&D Systems™",RnD systems,RnD systems Cat# Af2526 Goat anti-human polyclonal antibody InVivoMAb mouse IgG1 isotype control,BioXCell,BioXCell Cat#BE0083

(6) IL-21-Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis BFrontiers in immunologyJanuary 1, 2020Khanam A, Ayithan N, Tang L, Poonia B, Kottilil S.(1 µg/ml) (MT216G/21.3m Product code 3540-0N-500, MABTECH) and anti-human IL-27 (1 µg/ml) (Cat no. AF2526, R&D systems) neutralizing antibodies alone and in combination. At the end of co-culture, supernatant

(7) Type I IFN augments IL-27-dependent TRIM25 expression to inhibit HBV replicationCellular & molecular immunologyMarch 1, 2018Tan G, Xiao Q, Song H, Ma F, Xu F, Peng D, Li N, Wang X, Niu J, Gao P, et al.

(8) RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody productionPLoS pathogensNovember 1, 2017Sprokholt JK, Kaptein TM, van Hamme JL, Overmars RJ, Gringhuis SI, Geijtenbeek TBH.SDM25N-treated DCs to maintain inhibition of DENV replication. Neutralizing antibodies against IL-27 (5 μg/ml, AF2526; R&D Systems) or normal goat IgG (AB-108-C; R&D Systems) as isotype control was added at the start of

(9) HIV and HIV-Tat inhibit LPS-induced IL-27 production in human macrophages by distinct intracellular signaling pathwaysJournal of leukocyte biologySeptember 1, 2017Gajanayaka N, O'Hara S, Konarski Y, Fernandes J, Muthumani K, Kozlowski M, Angel JB, Kumar A.

(10) Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4Scientific reportsMarch 24, 2017Garg A, Trout R, Spector SA.

(11) Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activationPloS oneJanuary 1, 2017Gregersen I, Sandanger Ø, Askevold ET, Sagen EL, Yang K, Holm S, Pedersen TM, Skjelland M, Krohg-Sørensen K, Hansen TV, et al.

(12) IL-27 and type 2 immunity in asthmatic patients: association with severity, CXCL9, and signal transducer and activator of transcription signalingThe Journal of allergy and clinical immunologyFebruary 1, 2015Xie M, Mustovich AT, Jiang Y, Trudeau JB, Ray A, Ray P, Hu H, Holguin F, Freeman B, Wenzel SE.

(13) Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 productionNature communicationsOctober 3, 2014Gringhuis SI, Kaptein TM, Wevers BA, van der Vlist M, Klaver EJ, van Die I, Vriend LE, de Jong MA, Geijtenbeek TB.rhIL-27, 40 ng ml 1 rhIL-6 (both R&D Systems) or 5 mg ml 1 neutralizing antibodies against IL-27 (AF2526; R&D Systems), IL-6 (AF-206-NA; R&D Systems) or normal goat IgG (AB-108-C; R&D Systems), as a control,

(14) Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosusThe Journal of clinical investigationDecember 1, 2013Price JV, Haddon DJ, Kemmer D, Delepine G, Mandelbaum G, Jarrell JA, Gupta R, Balboni I, Chakravarty EF, Sokolove J, et al.14-7139-81, eBioscience; anti–IL-17A mAb, catalog no. 14-7178-81, eBioscience; anti–IL-27 mAb, catalog no. AF2526, R&D Systems; anti–MCP-1 mAb, catalog no. 14-7099-81, eBioscience; anti-TNF mAb, catalog no. 14-7348-81,

(15) Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitisJournal of immunology (Baltimore, Md. : 1950)April 1, 2013Fitzgerald DC, Fonseca-Kelly Z, Cullimore ML, Safabakhsh P, Saris CJ, Zhang GX, Rostami A.

(16) B cells as a therapeutic target for IFN-β in relapsing-remitting multiple sclerosisJournal of immunology (Baltimore, Md. : 1950)April 1, 2011Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S.

(17) Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27Journal of immunology (Baltimore, Md. : 1950)December 1, 2010Kalliolias GD, Gordon RA, Ivashkiv LB.

(18) IFN-beta inhibits human Th17 cell differentiationJournal of immunology (Baltimore, Md. : 1950)October 15, 2009Ramgolam VS, Sha Y, Jin J, Zhang X, Markovic-Plese S.IL-27p28 were neutralized with blocking Abs (polyclonal goat anti-human IgG Abs; R&D Systems catalog no. AF2526) at 10 ␮g/ml. Anti-CD3 plus anti-CD28 mAb-activated CD4⫹CD45RA⫹ T cells were cultured with 250 ␮l of

(19) Skin Injury Activates a Rapid TRPV1-Dependent Antiviral Protein ResponseThe Journal of investigative dermatologyJanuary 1, 0001"Lei V, Handfield C, Kwock JT, Kirchner SJ, Lee MJ, Coates M, Wang K, Han Q, Wang Z, Powers JG, et al."were induced using 30 small scissor cuts and 30 scalpel punctures. Human neutralizing IL-27 antibody (AF2526, R&D Systems) or normal goat IgG (AB-108-C, R&D Systems) in 500μL DMEM at 10μg/mL was added dropwise

(20) IL-27 promotes decidualization via the STAT3-ESR/PGR regulatory axisJournal of reproductive immunologyJanuary 1, 0001"Zhang XY, Shen HH, Qin XY, Wang CJ, Hu WT, Liu SP, Wu JN, Xie F, Xu FY, Zhao SM, et al."

Be the First to Write a Review!

Human IL-27 Antibody

IL-27 Antibody

IL-27 Antibody

IL-27 Antibody

IL-27 Antibody

IL-27 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (20)

Reviews

IL-27 Antibody from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map