MMR/CD206 Antibody AF2534 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMMR/CD206 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF2534
NCBI Gene AliasesCLEC13D, hMR, MMR, CLEC13DL, CD206, bA541I19.1, MRC1L1
ApplicationsBioassay,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry,Functional Assay,ELISA Capture
Quantity100 ug (also 25 ug)
HostGoat
TargetMMR/CD206
ReactivityHuman,Mouse,Primate - Macaca mulatta (Rhesus Macaque),Rat,Transgenic Mouse
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
NCBI Full Gene Namemannose receptor C-type 1
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human MMR/CD206, Leu19-Lys1383 (Thr399Ala) & (Leu407Phe), Accession # P22897
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MMR is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study macrophage polarization in human tissues?

Yes, the MMR/CD206 antibody has been utilized in studies to identify macrophage populations, particularly in distinguishing between M1 and M2 phenotypes. For instance, it has been employed in immunofluorescence staining to evaluate CD206 expression in human skeletal muscle, indicating its relevance in macrophage characterization in various tissue contexts .

Is the MMR/CD206 antibody effective for use in immunohistochemistry?

The MMR/CD206 antibody has been shown to work effectively in immunohistochemistry applications. It has been cited in studies where it was used to stain human tissue sections, demonstrating its utility in visualizing macrophage populations in various pathological conditions .

What species can this antibody detect, and is there any cross-reactivity?

The MMR/CD206 antibody is reactive with human, mouse, rat, and non-human primate tissues. It exhibits approximately 20% cross-reactivity with recombinant mouse MMR in direct ELISAs, which may be relevant for studies involving mouse models .

Has this antibody been referenced in studies related to liver function or disease?

Yes, the MMR/CD206 antibody has been referenced in studies examining liver sinusoidal endothelial cells and their scavenger functions in models of liver disease. It was used to assess the expression of the mannose receptor in these cells, highlighting its role in liver pathology .

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Cellular Senescence Links Muscle Atrophy and Posttraumatic Osteoarthritis after ACL InjuryFunction (Oxf)August 1, 2026Alexander R. Keeble, Allison M. Owen, Sara Gonzalez-Velez, Nicholas T. Thomas, Camille R. Brightwell, Peyton J. Balawender, Madeline G. O’Daniel, Helena C. Weiss, Haley Noehren, Heather Thompson, et al.streptavidin/biotin blocking (Vector Laboratories, #SP-2002) before incubation with CD206 (1:100, R&D Systems, #AF2534). On the final day, slides were incubated with biotinylated rabbit anti-goat IgG (1:500, Vector, #BA-5000)

(2) Interleukin-34–Induced Arg1+ Macrophages Play a Key Role in Breast Cancer Brain MetastasisCancer Res CommunJune 1, 2026Xiaoqing Cheng, Khooshbu K. Patel, Brandon Zhou, Yiwei Fu, Ryan T. Cleary, Maureen Highkin, Jacob Hsia, Xiaohua Jin, Benjamin Kohn, Julie L. Prior, et al.IL34 antibody (R&D Systems, AF5195, RRID: AB_2124393), and human CD206 antibody (R&D Systems, cat. #AF2534, RRID: AB_2063019). The secondary antibodies for IF include anti-rabbit IgG (H+L) Alexa Fluor 555 (Invitrogen,

(3) A longitudinal single-cell and spatial multiomic atlas of pediatric high-grade gliomaCell Reports MedicineMay 19, 2026Jonathan H. Sussman, Derek A. Oldridge, Wenbao Yu, Chia-Hui Chen, Abigail M. Zellmer, Jiazhen Rong, Arianne Parvaresh-Rizi, Anusha Thadi, Austin Yang, Joseph S. Tumulty, et al.AB_2798001 Anti-SOX4 (clone R807.1.1D6),CDI Labs,MAB409325 Anti-CD206 (clone polyclonal),R&D Systems,AF2534; RRID: AB_2063019 Anti-P53 (clone E26),Abcam,ab225531; RRID: AB_3718659 Anti-CD68 (clone KP1),Akoya,4550113;

(4) Telomerase Knockout in Myeloid Cells Predisposes Mice to Foam Cell Formation, Dyslipidemia, Lung Fibrosis, and Cardiac DysfunctionAging CellApril 16, 2026Zhanguo Gao, Yongmei Yu, David Wiggins, Eva M. Sevick-Muraca, Mikhail G. Koloninet al. 2020). For immunofluorescence (IF), the following antibodies were used: CD206 (R&D Systems, AF2534, 1:75); CD80 (Abclonal A23688, 1:50); CD68 (Invitrogen, MA5‐1324, 1:75); F4/80 (Abcam, ab16911, 1:50);

(5) Robust immune cell infiltration and macrophage senescence occur within a week of recovery after limb immobilization in older adult skeletal muscleJ PhysiolMarch 27, 2026Chad M. Skiles, Zachary J. Fennel, Paul‐Emile Bourrant, Elena M. Yee, Robert J. Castro, Hannah A. Zabriskie, Melina Itinose, Mark A. Supiano, Ryan M. O'Connell, Micah J. DrummondThermo Fisher Scientific, MA, USA) and CD68+ (catalog. no. 14‐068‐82; Thermo Fisher Scientific) CD206+ (catalog. no. AF2534; R&D Systems) macrophages; senescent macrophages (CD11b+; catalog. no. MON1019‐1; Cell Sciences, Newburyport,

(6) Robust immune cell infiltration and macrophage senescence occur within a week of recovery after limb immobilization in older adult skeletal muscleThe Journal of PhysiologyMarch 27, 2026Chad M. Skiles, Zachary J. Fennel, Paul‐Emile Bourrant, Elena M. Yee, Robert J. Castro, Hannah A. Zabriskie, Melina Itinose, Mark A. Supiano, Ryan M. O'Connell, Micah J. DrummondThermo Fisher Scientific, MA, USA) and CD68+ (catalog. no. 14‐068‐82; Thermo Fisher Scientific) CD206+ (catalog. no. AF2534; R&D Systems) macrophages; senescent macrophages (CD11b+; catalog. no. MON1019‐1; Cell Sciences, Newburyport,

(7) Plasma Extracellular Vesicle Modulate Immune Cell Transcriptional Responses Following Acute Myocardial InfarctioniScienceMarch 20, 2026Lewis Timms, Raphaella Ridley, Adam Braithwaite, Charlotte Hooper, Tanvir Rafe, Carolin Lewe, Sebastian Lemke, Divya Ganesh, Zeyu Chen, Tafadzwa Kufazvinei, et al.,BD Biosciences,"555404, RRID:AB_395804" CD162/PSGL,R&D Systems,AF3345; RRID:AB_10973837 CD206/MMR,R & D Systems,AF2534; RRID:AB_2063019 CD24,BD Biosciences,555426; RRID:AB_395820 CD3,BD Biosciences,555337; RRID:AB_395743 CD31

(8) Progesterone-driven stabilization of hybrid E/M states in amniotic epithelial cells enhances regeneration and immune modulatory capacitiesiScienceMarch 20, 2026Angelo Canciello, Alberto Maria Crovace, Verdiana Di Giulio, Giuseppe Prencipe, Mohammad El Khatib, Valentina Russo, Marta Guadalupi, Annunziata Mauro, Antonio Crovace, Oriana Di Giacinto, et al.RRID: AB_303415 Von Willebrand Factor,Dako,Cat# A0082; RRID: AB_2315602 Anti-CD206,RD System,Cat# AF2534; RRID: AB_2063019 Anti-CD86,Novus Biologicals,Cat# NBP2-25208; RRID: AB_2923115 Anti-rabbit Alexa

(9) Low-frequency ultrasound reverses insulin resistance and diabetes-induced changes in the muscle transcriptome in aged miceAmerican Journal of Physiology-Endocrinology and MetabolismJune 1, 2025Erik D. Marchant, Ekta Singh, Sanjay Kureel, Brandon Blair, Hanna Kalenta, Zachary D. Von Ruff, Korri S. Weldon, Zhao Lai, Michael P. Sheetz, Blake B. Rasmussenstreptavidin/biotin blocking, and 2.5% normal horse serum (1 hour). Goat anti-CD206 antibody (1:200; R&D Systems; AF2534) in 2.5% normal horse serum was applied overnight at 4°C. Sections were washed in PBS and incubated

(10) Malignant mesothelioma-associated inflammatory microenvironment promotes tumor progression via GPNMBJournal of translational medicineApril 18, 2025Belgiovine C, Digifico E, Erreni M, Putignano AR, Mannarino L, Valentino S, Grizzi F, Pasqualini F, Recordati C, Bertola L, et al.pathological archive; 3-µm sections were stained with primary antibodies: hGPNMB (BAF2330,) or hCD206 (AF2534) all from R&D System. RNAscope ISH technology was performed with GPNMB RNA-specific probes (RNAscope™

(11) Prognostic significance of cytotoxic-T-lymphocytes to immunosuppressive lymphocytes ratio (CIL) in laryngeal squamous cell carcinomaCancer Immunol ImmunotherMarch 24, 2025Duo Zhang, Yu Heng, Qiu-Yan Jin, Di Tang, Xiao-Ke Zhu, Li-Ming Lu, Chun-Ping Wu, Lei Taoanti-CD68 primary antibody (1:200, 76,437, clone D4B9C, CST) or goat monoclonal anti-CD206 (1:100, AF2534, R&D Systems) were incubated overnight at 4 °C. Following three 10-min washes with PBS, the sections

(12) Leishmania braziliensis enhances monocyte responses to promote anti-tumor activityCell reportsMarch 26, 2024Dos Santos JC, Moreno M, Teufel LU, Chilibroste S, Keating ST, Groh L, Domínguez-Andrés J, Williams DL, Ma Z, Lowman DW, et al.

(13) Graph Fourier transform for spatial omics representation and analyses of complex organsResearch squareFebruary 15, 2024Chang Y, Liu J, Jiang Y, Ma A, Yeo YY, Guo Q, McNutt M, Krull J, Rodig SJ, Barouch DH, et al.

(14) Skeletal muscle regeneration failure in ischemic-damaged limbs is associated with pro-inflammatory macrophages and premature differentiation of satellite cellsGenome medicineNovember 10, 2023Southerland KW, Xu Y, Peters DT, Lin X, Wei X, Xiang Y, Fei K, Olivere LA, Morowitz JM, Otto J, et al.room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences, MON1019-1, clone Bear-1), and anti-dystrophin (Thermo Scientific, RB-9024-P).

(15) M1/M2 Macrophage Skewing is Related to Reduction in Types I, V, and VI Collagens with Aging in Sun-Exposed Human SkinJID InnovationsNovember 1, 2023Satoshi Horiba, Munetaka Kawamoto, Ryozo Tobita, Ryota Kami, Yuki Ogura, Junichi Hosoipreviously (Horiba et al., 2022). The samples were stained with primary antibodies against CD206 (1:200, AF2534, R&D Systems, Minneapolis, MN), CD68 (1:100, ab955, Abcam, Cambridge, United Kingdom), CD86 (1:50, AF141NA,

(16) Pro-inflammatory macrophages impair skeletal muscle regeneration in ischemic-damaged limbs by inducing precocious differentiation of satellite cellsbioRxiv : the preprint server for biologyApril 3, 2023Southerland KW, Xu Y, Peters DT, Wei X, Lin X, Xiang Y, Fei K, Olivere LA, Morowitz JM, Otto J, et al.

(17) CD206(+) tumor-associated macrophages interact with CD4(+) tumor-infiltrating lymphocytes and predict adverse patient outcome in human laryngeal squamous cell carcinomaJournal of translational medicineMarch 3, 2023Heng Y, Zhu X, Lin H, Jingyu M, Ding X, Tao L, Lu L.were diluted as follows: Anti-CD68 antibody (76437, clone D4B9C, CST), 1:200; Anti-MMR/CD206 antibody (AF2534, R&D Systems), 1:100; Anti-iNOS antibody (610328, BD Biosciences), 1:100; Anti-CD163 antibody (ab182422,

(18) Important functional role of the protein osteopontin in the progression of malignant pleural mesotheliomaFrontiers in immunologyJanuary 1, 2023Digifico E, Erreni M, Mannarino L, Marchini S, Ummarino A, Anfray C, Bertola L, Recordati C, Pistillo D, Roncalli M, et al.paraffin-embedded human tissues were stained with primary antibodies anti-OPN (MAB14334, R&D System) or anti-CD206 (AF2534,R&D System). For murine tissues, 4-μm sections of paraffin-embedded lungs were stained as previously

(19) Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmpgt/gt mouse model of slowly progressing liver fibrosisPloS oneJanuary 1, 2023Antwi MB, Dumitriu G, Simón-Santamaria J, Romano JS, Li R, Smedsrød B, Vik A, Eskild W, Sørensen KK.R&D Systems, Cat. No AF1460; working concentration: 4 μg/ml), goat anti-human MMR/CD206 (R&D Systems, Cat. No AF2534; 5 μg/ml), rabbit anti-human stabilin-1 (Atlas, Cat. No HPA005434; 3 μg/ml), goat anti-mouse VSig4 (Bio-Techne,

(20) Tissue Levels of CD80, CD163 and CD206 and Their Ratios in Periodontal and Peri-Implant Health and DiseaseCurrent issues in molecular biologyOctober 10, 2022Yilmaz M, Demir E, Firatli Y, Firatli E, Gürsoy UK.incubated with the primary antibodies (1:500 dilution; B7-1/CD80: #AF140, CD163: #AF1607, MMR/CD206: #AF2534; R&D Systems, Bio-Techne, USA) overnight. Then, the membranes were washed and incubated with HRP-conjugated

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12/18/2019Good Antibody to Stain M2 Microglial Polarization Phenotype Abhijit NirwaneStains CD206-expressing microglia.