Immunocytochemical analysis of Drosha in LOVO cells using a Drosha Monoclonal antibody (Product # MA5-32818) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western blot analysis was performed on nuclear enriched cell extract (30 µg lysate) of K-562 (Lane 1), and HeLa (Lane 2). The blot was probed with Anti-Drosha Monoclonal Antibody (Product # MA5-32818, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 170 kDa band corresponding to Drosha was observed in all cell lines tested with *non-specific bands at ~260 kDa and ~110 kDa.