Fig 1: Renal TNF-a, TNFR1 and TNFR2 immunostaining. Representative images of immunostaining for TNF-a, TNFR1 and TNFR2 in the renal cortex and medulla of rat groups under study (400×). The scale bar indicated in each image represents 50µm. (A1), negative reaction for TNF-a; (A2), TNF-a immunostaining in sham group; (A3), TNF-a immunostaining in mild CRF group; (A4), TNF-a immunostaining in moderate CRF group; (B1), negative reaction for TNFR1; (B2), TNFR1 immunostaining in sham group; (B3), TNFR1 immunostaining in mild CRF group; (B4), TNFR1 immunostaining in moderate CRF group; (C1), negative reaction for TNFR2; (C2), TNFR2 immunostaining in sham group; (C3), TNFR2 immunostaining in mild CRF group; (C4), TNFR2 immunostaining in moderate CRF group.