CD36 Antibody MA5-32433 from Thermo Fisher Scientific

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Western Blot was performed using Anti-CD36 Recombinant Rabbit Monoclonal Antibody (JJ2005) (Product # MA5-32433) and an 88 kDa band corresponding to CD36 was observed. Expression was upregulated in THP-1 differentiated cells using PMA and the expression was reduced in Jurkat when compared to THP-1. Relative expression was seen in adipose, brown adipose with higher expression when compared to other tissues. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with PMA (80 nm, 16 hrs) (Lane 2), Jurkat (Lane 3), RAW 264.7 (Lane 4), Hep G2 (Lane 5), K-562 (Lane 6) in Fig. a; tissue extracts of Mouse Adipose (lane 1), Mouse Brown Adipose (Lane 2), Rat Adipose (Lane 3), Rat Brown Adipose (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6), Mouse Kidney (Lane 7), Mouse Liver (Lane 8), Rat Testis (Lane 9), Mouse Cerebellum (Lane 10), Mouse Colon (Lane 11) in Fig. b, were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1,000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6,000 dilution) using the iBright FL 1000 (Product # A32752).
ICC/IF

Immunofluorescence analysis of Platelet glycoprotein 4 was performed using 70% confluent log phase THP-1 and THP-1 cells differentiated using PMA (80 nm for 16hrs) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD36 Recombinant Rabbit Monoclonal Antibody (JJ2005) (Product # MA5-32433) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane localization. Panel e represents control THP-1 cells with lower expression levels. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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Thermo Fisher Scientific for
CD36 Antibody

The CD36 Antibody from Thermo Fisher Scientific is a Rabbit Monoclonal antibody to CD36, and CD36 molecule. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The CD36 Antibody has been shown to work in the following applications: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.

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CD36 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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CD36 Antibody from Thermo Fisher Scientific

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