Immunocytochemistry-Immunofluorescence analysis of RBM25 was performed in U-2 OS cells using RBM25 Polyclonal Antibody (Product # PA5-90511).

Western blot analysis of RBM25 in extracts of various cell lines. Samples were incubated with RBM25 Polyclonal antibody (Product # PA5-90511) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.