Flow cytometric analysis of Mouse CD8α expression on BABL/c splenocytes. Cells were stained with CD8 alpha Recombinant Rabbit Monoclonal Antibody (208) (Product # MA5-29682), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Mouse CD8a in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD8 alpha Recombinant Rabbit Monoclonal Antibody (208) (Product # MA5-29682, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.
Supplier Page from Thermo Fisher Scientific for CD8 alpha Antibody