Immunofluorescence analysis of PMS2 was performed using 70% confluent log phase HeLa and DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. HeLa cells were labeled with PMS2 Rabbit Monoclonal Antibody (Product # MA5-32044) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image of HeLa showing nuclear localization. Panel e represents the merged image of DU 145 cells showing no expression for PMS2 protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.