Western blot was performed using Anti-LGR5 Recombinant Rabbit Monoclonal Antibody (SU32-04) (Product # MA5-32108) and a 100kDa band corresponding to LGR5 was observed. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Mouse Heart (Lane 2) and Rat Skeletal Muscle (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1,000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Relative expression was observed between Mouse Skeletal muscle and Mouse Heart , a tissue which is reported to express LGR5 at lower levels compared to Skeletal Muscle.