Immunofluorescence staining of CD34 in M1 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD34 Recombinant Rabbit Monoclonal Antibody (13) (Product # MA5-29674, 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

CD34 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate 0.5 µL with CD34 Recombinant Rabbit Monoclonal Antibody (13) (Product # MA5-29674) and 15 µL of 50 % Protein G agarose. Primary antibody: CD34 Recombinant Rabbit Monoclonal Antibody (13), at 1:350 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 130 kDa.