TOMM20 Antibody MA5-32148 from Thermo Fisher Scientific

Product Specs
ItemTOMM20 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberMA5-32148
Quantity100 µL
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Concentration1 mg/mL
Conjugate/TagUnconjugated
FormatTBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
PurityProtein A
ReactivityHuman, Mouse, Rat
TargetTOMM20
IsotypeIgG
CloneST04-72
HostRabbit
ImmunogenRecombinant protein within Human TOMM20 aa 1-145
NCBI Full Gene Nametranslocase of outer mitochondrial membrane 20
NCBI Gene AliasesMAS20, 1810060K07Rik, mKIAA0016, Gm19268, MOM19, TOM20
ClonalityRecombinant Monoclonal
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Mitochondrial oxidative stress promotes the accumulation of advanced glycation end productsPLoS OneJune 23, 2026Firoz Akhter, Sourav Samanta, Alexandre A. Sosunov, Huawei Wu, Ethan Kim Tieu, Shi Fang Yan, Shirley ShiDu Yan5% goat serum for 60 min. Cells were then incubated with primary antibodies, TOMM20 (Thermo Fisher, MA5–32148, 1:200) or AGE (Antibodies-online, ABIN738516, 1:250). Following several washes with PBS, cells were

(2) Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translationNucleic Acids ResApril 30, 2026Dorthy Fang, John M Babich, Ryan Stanton, Isaac W Vock, Kyrillos Abdallah, Mingyi Zhu, Raj Letchuman, Richard Li, Matthew D Simon, Wendy V Gilbert, et al.anti-beta-tubulin at 1:1000 dilution (Cell Signaling, #86298), and anti-TOMM20 at 1:1000 dilution (Invitrogen, MA532148). Donkey anti-rabbit IgG DyLight 800 secondary antibody and donkey anti-mouse IgG DyLight 680 secondary

(3) Müller Glial Kir4.1 Channel Dysfunction in APOE4 ‐ KI Model of Alzheimer's DiseaseGliaMarch 1, 2026Surabhi D. Abhyankar, Yucheng Xiao, Neha Mahajan, Qianyi Luo, Theodore R. Cummins, Adrian L. Oblak, Bruce T. Lamb, Timothy W. Corson, Ashay D. Bhatwadekar#APC‐035‐GP, Alomone Labs, 1:200), glutamine synthetase (GS, Cat. #MAB302, Millipore, 1:200), TOMM20 (Cat. #MA5‐32148, Invitrogen, 1:100) and Aquaporin 4 (AQP4, Cat. #sc‐ 32,739, Santa Cruz Biotechnology Inc., TX, USA,

(4) The intermediate filament protein GFAP regulates mitochondrial fission in astrocytesProceedings of the National Academy of SciencesDecember 2, 2025Ding Xiong, Ye Sing Tan, Fang Yuan, Yinglu Li, Kay En Low, Isabelle Bonne, Sakthikumar Mathivanan, Phil Jun Kang, Zijun Sun, Xueyan Li, et al.goat anti-Sox9 (R&D, AF3075), rabbit anti-S100β (Abcam, ab52642), rabbit anti-Tomm20 (Thermofisher, MA532148), rabbit anti-Drp1 (Cell signaling technology, 5391), rabbit anti-Drp1 (Cell signaling technology, 8570),

(5) Roles of 670 nm Photobiomodulation on Rat Anterior Ischemic Optic Neuropathy: Enhancing RGC Survival, Mitochondrial Function, and Anti-Inflammatory ResponseAntioxidants (Basel)July 18, 2025Tu-Wen Chen, Yao-Tseng Wen, Pei-Kang Liu, Monir Hossen, Rong-Kung Tsai-8-OHdG (1:200, Catalog # SAB5200010, Sigma-Aldrich, St. Louis, MO, USA), and TOMM20 (1:200; Catalog # MA5-32148, Invitrogen™, Eugene, OR, USA) were used in their procedure. The immunohistochemistry protocol was described

(6) TANGO2 binds crystallin alpha B and its loss causes desminopathyNat CommunJune 6, 2025Maike Stentenbach, Laetitia A. Hughes, Samuel V. Fagan, Blake Payne, Danielle L. Rudler, Stefan J. Siira, Tim McCubbin, Anaëlle Chopin, Kara L. Perks, Judith A. Ermer, et al.(3932, Cell signalling, diluted 1:1000), SH3GL2 (PA5-120939, Invitrogen, diluted 1:500) and TOMM20 (MA5-32148, Invitrogen, diluted 1:1000) and mouse antibodies against p62 (ab56416, Abcam, diluted 1:500), OPA1

(7) Therapeutic Efficacy of Small Extracellular Vesicles Loaded with ROCK Inhibitor in Parkinson's DiseasePharmaceuticsMarch 13, 2025Candy Carbajal, Myosotys Rodriguez, Florida Owens, Nicole Stone, Dileepkumar Veeragoni, Rebecca Z. Fan, Kim Tieu, Nazira El-Hageab23707), which were procured from Abcam(Waltham, MA, USA), and used at 1:1000 dilution. TOM 20 (catalog # MA5-32148), obtained from Invitrogen (Carlsbad, CA, USA); Caspase 3 (catalog # 9665), from Cell Signaling Technology

(8) Functional large-conductance calcium and voltage-gated potassium channels in extracellular vesicles act as gatekeepers of structural and functional integrityNat CommunJanuary 2, 2025Shridhar Sanghvi, Divya Sridharan, Parker Evans, Julie Dougherty, Kalina Szteyn, Denis Gabrilovich, Mayukha Dyta, Jessica Weist, Sandrine V. Pierre, Shubha Gururaja Rao, et al.(Alomone labs, #APC21), anti-Na-K-ATPase (Abcam, #ab7671), anti-TOMM20 (Thermo Fisher Scientific, #MA5-32148), anti-ALIX (Cell Signaling Technology, #2171S), anti-GAPDH (Cell Signaling Technology, #2118), anti-CD-81

(9) Reduced bioenergetics and mitochondrial fragmentation in human primary cytotrophoblasts induced by an EGFR-targeting chemical mixtureChemosphereSeptember 7, 2024Anita A. Waye, Elvis Ticiani, Zinat Sharmin, Vanessa Perez Perez Silos, Thilini Perera, Alex Tu, Irina A. Buhimschi, Carlos A. Murga-Zamalloa, Ying S. Hu, Almudena Veiga-LopezX-100 0.05%) for 90 min. Thereafter, the cells were incubated with the primary antibody TOMM20 (Cat#: MA5-32148, Thermo Fisher Scientific, Rockford, IL, USA) in blocking buffer at 5 μg/ml and incubated for 60 min

(10) Inflammatory lung injury is associated with endothelial cell mitochondrial fission and requires the nitration of RhoA and cytoskeletal remodelingFree Radical Biology and MedicineAugust 20, 2024Marissa D. Pokharel, Panfeng Fu, Alejandro Garcia-Flores, Manivannan Yegambaram, Qing Lu, Xutong Sun, Hoshang Unwalla, Saurabh Aggarwal, Jeffrey R. Fineman, Ting Wang, et al.in PBS for 1 hour and incubated with the TOMM20 antibody overnight at 4°C (red, ThermoFisher, Cat # MA5–32148). After the overnight incubation with the primary antibody, the secondary antibody was added for 1 hour

(11) Single-Molecule Localization Microscopy Using Time-Lapse Imaging of Single-Antibody LabelingCurr ProtocOctober 1, 2023Perera T, Gunasekara H, Hu YS.RT0268, 25 mg)",HA tag on α-tubulin (microtubule),67 "Rabbit polyclonal Tom20 (Thermo Fisher Scientific, MA5-32148)",Tom20 (mitochondria),27 Unconjugated antibodies used for IF staining.

(12) Proximity-labeling chemoproteomics defines the subcellular cysteinome and inflammation-responsive mitochondrial redoxomeCell chemical biologyJuly 20, 2023Yan T, Julio AR, Villanueva M, Jones AE, Ball AB, Boatner LM, Turmon AC, Nguyễn KB, Yen SL, Desai HS, et al.

(13) Altered Mitochondrial Opa1-Related Fusion in Mouse Promotes Endothelial Cell Dysfunction and AtherosclerosisAntioxidants (Basel, Switzerland)May 28, 2022Chehaitly A, Guihot AL, Proux C, Grimaud L, Aurrière J, Legouriellec B, Rivron J, Vessieres E, Tétaud C, Zorzano A, et al.Mitochondria were visualized using TOMM20 recombinant rabbit monoclonal antibody (Invitrogen™, cat#MA5-32148, 1/200, Eugene, OR, USA) coupled with donkey anti-rabbit IgG Alexa Fluor 568, Invitrogen™ (cat#A10042,

(14) Mitochondrial fission links ECM mechanotransduction to metabolic redox homeostasis and metastatic chemotherapy resistanceNature cell biologyFebruary 1, 2022Romani P, Nirchio N, Arboit M, Barbieri V, Tosi A, Michielin F, Shibuya S, Benoist T, Wu D, Hindmarch CCT, et al.

(15) Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout MiceFrontiers in cellular neuroscienceJanuary 1, 2021Cibelli A, Veronica Lopez-Quintero S, Mccutcheon S, Scemes E, Spray DC, Stout RF Jr, Suadicani SO, Thi MM, Urban-Maldonado M.to stain nuclei). To co-label mitochondria we stained with TOMM20 primary antibody (Invitrogen, cat# MA5-32148, 1:500) followed by secondary staining with Donkey anti-rabbit Alexa Fluor 647 (Figure 6B, Invitrogen,

(16) Repeat intravital imaging of the murine spinal cord reveals degenerative and reparative responses of spinal axons in real-time following a contusive SCIExperimental neurologyMay 1, 2020Rajaee A, Geisen ME, Sellers AK, Stirling DP.polyglutamylation respectively, the following primary antibodies were used: monoclonal anti-TOMM20 (1:50, MA5–32148, Invitrogen) and monoclonal anti-tubulin, polyglutamylated antibody (1:100, T9822, Millipore Sigma).

(17) Restriction of mitochondrial calcium overload by mcu inactivation renders a neuroprotective effect in zebrafish models of Parkinson's diseaseBiology openOctober 15, 2019Soman SK, Bazała M, Keatinge M, Bandmann O, Kuznicki J.used: TH [mouse monoclonal IgG1, #ABIN617906, (Antibodies Online, 1:1000)], tom20 [Rabbit monoclonal, #MA5–32148, (Thermo Fisher Scientific, 1:500)]. The following Alexa Flour secondary antibodies were used: Alexa

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TOMM20 Antibody from Thermo Fisher Scientific

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TOMM20 Antibody from Thermo Fisher Scientific

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