Knockdown of TP63 was achieved by transfecting A431 cells with specific siRNA (Silencer® select Product # s13309, s13311). Immunofluorescence analysis was performed on A431 cells (untransfected, panel a-d), transfected with TP63 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-TP63 Recombinant Rabbit Monoclonal Antibody (Product # 703809, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of the signal was observed upon siRNA mediated knockdown (panel i-l) confirming the specificity of the antibody to TP63 (green). The images were captured at 60X magnification.