Western blot was performed using Anti-CD20 Recombinant Rabbit Monoclonal Antibody (SY12-01) (Product # MA5-32050) and a ~33 kDa band corresponding to CD20 was observed in Daudi and Ramos but not in Jurkat, SP2/0-Ag14 ,and EL4 cell lines. Membrane enriched extracts (30 µg lysate) of Daudi (Lane 1), Ramos (Lane 2), Sp2/0-Ag14 (Lane 3), Jurkat (Lane 4) and EL4 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:5000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).