APAF1 Antibody MA5-32082 from Thermo Fisher Scientific

Supplier Images
Published Figures

WB

Western blot was performed using Anti-APAF1 Recombinant Rabbit Monoclonal Antibody (SY22-02) (Product # MA5-32082) and a 140 kDa band corresponding to APAF1 was observed across the cell lines and tissues tested with relatively lower levels in iPSC differentiated cardiomyocytes. Whole cell extracts (30 µg lysate) of THP1 (Lane 1), MDA-MB-231 (Lane 2), iPSC (Lane 3), iPSC differentiated into cardiomyocytes (Lane 4), tissue extracts (30 µg lysate) of Mouse Spleen (Lane 5) and Mouse Heart (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of APAF1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with APAF1 Recombinant Rabbit Monoclonal Antibody (Product # MA5-32082) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Be the first to write a review!

Citations:

(3)

Figures:

Supplier Page

Supplier Page from
Thermo Fisher Scientific for
APAF1 Antibody

Thermo Fisher Scientific's APAF1 Antibody is a Rabbit monoclonal antibody. The APAF1 Antibody was generated using APAF1 as the antigen. It reacts with Homo Sapiens, Human, and Mouse. This antibody has been shown to work in applications such as: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.

WB

ICC/IF

APAF1 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

Citations (3)

Reviews

APAF1 Antibody from Thermo Fisher Scientific

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map