Fig 1: miR-3148 targets DCUN1D1. (A) DCUN1D1 expression in glioma tissues and non-tumor tissues was compared and was found to be elevated in glioma tissues. (B) DCUN1D1 was expressed at a lower level in glioma cell lines compared with that in the HEB cell line. (C) The putative miRNA binding sites in the DCUN1D1 sequence. Dual-luciferase reporter was used to confirm the direct targeting between DCUN1D1 and miR-3148. (D) miR-3148 mimics were transfected into U251 cells, after which the mRNA level of DCUN1D1 was assessed using reverse transcription-quantitative PCR. (E) DCUN1D1 protein levels in U251 cells treated with miR-3148 mimics or NC were analyzed using western blotting, with GAPDH as control. (F) DCUN1D1 protein level in xenograft tumors was analyzed via immunohistochemistry (scale bar, 50 µm). Data are shown as mean ± SD. *P<0.05; **P<0.01; ***P<0.001. miR, microRNA; NC, negative control; WT, wild type; MUT, mutant.