Immunocytochemical analysis of CD49b (Integrin alpha 2) in MCF-7 cells using a CD49b (Integrin alpha 2) Monoclonal antibody (Product # MA5-32306) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western blot was performed using Anti-CD49b (Integrin alpha 2) Recombinant Rabbit Monoclonal Antibody (SN0752) (Product # MA5-32306) and bands at 160, 120 kDa corresponding to CD49b were observed across the cell lines and tissue tested except U-937 which is reported to be negative. Membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), T-47D (Lane 3), A-431 (Lane 4), U-2 OS (Lane 5), HEK-293 (Lane 6), A549 (Lane 7), U-937 (Lane 8) and Mouse Spleen (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).