Fig 1: The characteristics of terminal cardiomyocytes. A, Plot map shows the expression of genes involved in cell proliferation in the cardiomyocytes. B, Plot map shows the correlation of unique molecular identifiers (UMIs) and genes in each cell of terminal cardiomyocytes (tCMs). C, Comparison of the relative expression of the highest 300 genes in the 3 cardiomyocytes. D, Gene Ontology (GO) analyses of the 300 most highly expressed genes in the terminal cardiomyocytes. E, Monocle analyses showing the ordering of atrium cardiomyocyte (top), ventricle cardiomyocyte (bottom), and terminal cardiomyocyte (middle) along pseudo‐time trajectories. F, BioRender infographic summarizes the predicted relationship of the 3 cardiomyocyte clusters. G, Plot map shows the expression of genes involved in RNA polymerase II in the cardiomyocytes. H, Immunofluorescence (IF) of TTN (titin) and TAF1 (TATA‐box binding protein associated factor 1) in the atrium and ventricles: TTN (red, cardiomyocyte marker) and TAF1 (green, RNA polymerase II marker). Nuclei are counterstained with 4′,6‐diamidino‐2′‐phenylindole (DAPI) (blue). Scale bars, 50 μm. nGene indicates number of genes; nUMI, number of unique molecular identifiers; and RPS3, ribosomal protein S3.
Fig 2: The characteristics of terminal cardiomyocytes. A, Plot map shows the expression of genes involved in cell proliferation in the cardiomyocytes. B, Plot map shows the correlation of unique molecular identifiers (UMIs) and genes in each cell of terminal cardiomyocytes (tCMs). C, Comparison of the relative expression of the highest 300 genes in the 3 cardiomyocytes. D, Gene Ontology (GO) analyses of the 300 most highly expressed genes in the terminal cardiomyocytes. E, Monocle analyses showing the ordering of atrium cardiomyocyte (top), ventricle cardiomyocyte (bottom), and terminal cardiomyocyte (middle) along pseudo‐time trajectories. F, BioRender infographic summarizes the predicted relationship of the 3 cardiomyocyte clusters. G, Plot map shows the expression of genes involved in RNA polymerase II in the cardiomyocytes. H, Immunofluorescence (IF) of TTN (titin) and TAF1 (TATA‐box binding protein associated factor 1) in the atrium and ventricles: TTN (red, cardiomyocyte marker) and TAF1 (green, RNA polymerase II marker). Nuclei are counterstained with 4′,6‐diamidino‐2′‐phenylindole (DAPI) (blue). Scale bars, 50 μm. nGene indicates number of genes; nUMI, number of unique molecular identifiers; and RPS3, ribosomal protein S3.
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