Fig 1: Inefficient CENTRE leads to accumulation of protein aggregates. (A) Confocal imaging of the Myc-ER-GFP protein and mCherry-Sec61ß. HeLa cells depleted of the indicated proteins were transiently transfected with a plasmid expressing Myc-ER-GFP, a reference plasmid expressing mCherry-Sec61ß, and HA, CBP80R-WT-HA, or CBP80R-L34E-HA. Scale bar, 10 µm; n = 3. (B and C) PLA for CBP80 and SRP54. HeLa cells, either undepleted or depleted of SRa, were subjected to PLA (B) Quantitation of PLA signals for endogenous CBP80 and SRP54. More than 50 cells from three biological replicates were quantitated. (C) PLA images. The antibodies raised in a mouse or rabbit are colored in yellow or red, respectively. mIgG, nonspecific mouse IgG; rIgG, nonspecific rabbit IgG; scale bar, 10 µm; n = 3. (D) PLA for eIF4E and SRP54. A previously known interaction between Y14 and MAGOH served as a positive control; n = 3; scale bar, 10 µm.