Ribosomal Protein S3 (D50G7) XP ® Rabbit mAb 9538S from Cell Signaling Technology

Product Specs
ItemRibosomal Protein S3 (D50G7) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number9538S
Quantity100 µl
ApplicationsW, IP, IF-IC
ReactivityH, M, R, Mk
TargetRPS3
HostRabbit
NCBI Gene AliasesS3, uS3
ClonalityMonoclonal
NCBI Full Gene Nameribosomal protein S3
Research AreaTranslational Control
IsotypeIgG
CloneD50G7
Molecular Weight27

Questions and Answers

What species does this antibody react with, and has it been used in relevant studies?

The Ribosomal Protein S3 (D50G7) XP® Rabbit mAb detects endogenous levels of total ribosomal protein S3 and shows reactivity with mouse, non-human primate, human, and rat. It has been cited in 19 scientific articles, indicating its utility in various research contexts.

Can this antibody be used in studies involving nutrient stress and ribosomal protein dynamics?

Yes, this antibody has been utilized in studies such as the one by Heeseon et al. (), which investigated ribosomal protein homeostasis during nutrient stress. The research highlighted the role of ribosomal proteins, including RPS3, in translational suppression and proteome remodeling under stress conditions.

Is there any evidence of this antibody being used in cancer research?

Yes, the antibody has been referenced in studies like Huang et al. (), where RPS3 was shown to interact with lipocalin 2, influencing NF-κB pathway activation and affecting cisplatin sensitivity in oral squamous cell carcinoma. This suggests its relevance in cancer biology.

How has this antibody been applied in the context of translation regulation?

The Ribosomal Protein S3 antibody has been employed in research examining translation dynamics, such as in the study by Ichihara et al. (), which explored the dependence of translation initiation on eIF2 and identified RPS3's role in translation initiation at near-cognate codons.

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Website: Technical Support

(1) Locally synthesized glycyl aminoacyl-tRNA synthetase is important for local translation in neuronsLife Sci AllianceApril 15, 2026Tyler Brent de Leon, Adi Golani-Armon, Bar Cohen, Yoav S Arava,Cell Signaling,#4542,1:250 NEFM,Abcam,ab7794,1:250 Puromycin,Abcam,ab315887,1:100 Puromycin,Developmental Studies Hybridoma Bank (DSHB),PMY-2A4,IF 1:10; Western 1:100 RPS3,Cell Signaling Technology,#9538,1:100 Antibodies.

(2) Depurination of sarcin/ricin loop 25S rRNA is signaled through the small ribosomal subunit during translationRNADecember 1, 2025Tanya Prashar, Katalin A. Hudak131505), monoclonal anti-ubiquitin (1:1000; Santa Cruz 8017), monoclonal anti-Rps3 (1:1000; Cell Signaling 9538), monoclonal anti-RpL3 (1:5000; provided by Dr. J.R. Warner), polyclonal anti-eEF2 (1:10,000; Kerafast

(3) A selective RPL15 PROTAC degrader enhances anti-PD-1 immunotherapy in a murine melanoma tumor modelOncogeneNovember 23, 2025Runa Takahashi, Kazuki Yamamoto, Hikaru Toya, Haruka Shoji, Kohei Kawanishi, Kyoka Momosaki, Miyuki Yabe, Ken Takashima, Ryuta Muromoto, Satoshi Ichikawa, et al.antibody (VPA00489KT, ×1000 dilution, Bio-Rad Laboratories, Hercules, CA, USA), anti-RPS3 antibody (#9538, D50G7, ×1000 dilution, CST), anti-p53 antibody (sc-6243, ×500 dilution, Santacruz), anti-p21 antibody

(4) Proximity interactome mapping furthers a role for spinophilin in protein homeostasisiScienceNovember 21, 2025Emily T. Claeboe, Keyana L. Blake, Nikhil R. Shah, Cameron W. Morris, Basant Hens, Brady K. Atwood, Sabrina Absalon, Amber L. Mosley, Emma H. Doud, Anthony J. BaucumBip (C50B12) Rabbit mAb,Cell Signaling,"3177, RRID:AB_2119845" Ribosomal Protein S3 (D50G7) XP® Rabbit mAb,Cell Signaling,"9538, RRID:AB_10622028" Recombinant anti ALFA mouse,(SYSY) Synaptic Systems,"N1582, RRID:AB_3065196" eGFP

(5) Dual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in the tumor microenvironmentNature communicationsMay 19, 2025Aviles-Huerta D, Del Pizzo R, Kowar A, Baig AH, Palazzo G, Stepanova E, Amaya Ramirez CC, D'Agostino S, Ratto E, Pechincha C, et al.cat. 1620115). Antibodies used were RPL10a (Abcam; cat. Ab174318, 1:1,000), RPS3 (Cell Signaling; cat. 9538, 1:1,000), eGFP (Santa Cruz; cat. sc-9996, 1:1,000), mCherry (Abcam; cat. Ab167453 1:1,000), Neongreen

(6) Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificityNature Cell BiologyMarch 1, 2025Joanna R. Kovalski, Goksu Sarioglu, Vishvak Subramanyam, Grace Hernandez, Gilles Rademaker, Juan A. Oses-Prieto, Macey Slota, Nimmy Mohan, Kaylee Yiakis, Isabelle Liu, et al.(Bethyl, A301–743A, 1:1000), anti-RPS12 (ProteinTech, 16490–1-AP, 1:1000), anti-RPS3 (Cell Signaling, 9538S, 1:1000), anti-RPS20 (ProteinTech, 15692–1-AP, 1:1000), anti-RPS8 (Bethyl, A305–016A, 1:1000), anti-

(7) Localized K63 Ubiquitin Signaling Is Regulated by VCP/p97 During Oxidative StressMolecular & Cellular ProteomicsMarch 1, 2025Austin O. Maduka, Sandhya Manohar, Matthew W. Foster, Gustavo M. Silvaubiquitin (1:5000, Cell Signaling Technology, 8081S), anti-RPS3/uS3 (1:10,000, Cell Signaling Technology, #9538S), anti-tubulin (1:7500, Novus Biologicals, MAB93441), anti-TRAPa (1:10,000, Abcam, ab240562), anti-puromycin

(8) Skeletal muscle ribosome analysis: A comparison of common assay methods and utilization of a novel RiboAb antibody cocktailPhysiol RepJanuary 7, 2025Joshua S. Godwin, J. Max Michel, C. Brooks Mobley, Gustavo A. Nader, Michael D. RobertsUSA, cat #: 14568S), 5 μL rpl11 (Cell Signaling, cat #: 18163S), 5 μL rps3 (Cell Signaling, cat #: 9538S), and 5 μL rps6 (Cell Signaling, cat #: 2217). These four antibodies were chosen based on a few criteria.

(9) Nuclear eIF1 release increases mitotic stringency of start codon selectionNatureNovember 14, 2024Jimmy Ly, Kehui Xiang, Kuan-Chung Su, Gunter B. Sissoko, David P. Bartel, Iain M. Cheesemanpaper), anti-eIF5 (1:2000, Proteintech, 11155–1-AP), anti-RPS3 (1:1000, Cell Signaling Technology, #9538), anti phospho-histone H3 (Ser10) (1:1000, Cell Signaling Technology, #9701), anti-CDC20 (1:200, Santa Cruz Biotechnology

(10) Redox control of the deubiquitinating enzyme Ubp2 regulates translation during stressThe Journal of biological chemistryNovember 1, 2024Santos CM, Cizubu BK, Okonkwo DA, Chen CY, Maske N, Snyder NA, Simões V, Washington EJ, Silva GM.anti-uL5/Rpl11 (1:6000; Cell Signaling, cat. #18163), anti-uS3/Rsp3 (1:6000; Cell Signaling, cat. #9538), anti-PGK1 (1:8000; Invitrogen, cat. #22C5D8), anti-GAPDH (1:3000, Abcam, cat. #ab9485), anti-HA (1:3000,

(11) ADAR3 modulates neuronal differentiation and regulates mRNA stability and translationNucleic Acids ResearchOctober 28, 2024Victor Karlström, Eduardo A Sagredo, Jordi Planells, Charlotte Welinder, Jennifer Jungfleisch, Andrea Barrera-Conde, Linus Engfors, Chammiran Daniel, Fátima Gebauer, Neus Visa, et al.Merck Millipore, 1:10 000), anti-phospho-eIF2α (Cell Signaling #3398 1:5000), anti-RPS3 (Cell Signaling #9538 1:1000), anti-YBX1 (Abcam #ab12148 1:100), anti-STAU1 (Abcam #137100 1:100) and anti-RPL7 (Abcam #ab72550

(12) Localized K63 ubiquitin signaling is regulated by VCP/p97 during oxidative stressbioRxivJune 23, 2024Austin O. Maduka, Sandhya Manohar, Matthew W. Foster, Gustavo M. Silvaanti-K48 ubiquitin (1:5,000, Cell Signaling Technology, 8081S), anti-RPS3/uS3 (1:10,000, Cell Signaling, #9538S), anti-tubulin (1:7,500, Novus Biologicals, MAB93441), anti-TRAPa (1:10,000, Abcam, ab240562), anti-puromycin

(13) Polyglutamine-mediated ribotoxicity disrupts proteostasis and stress responses in Huntington’s diseaseNature Cell BiologyJune 1, 2024Ranen Aviner, Ting-Ting Lee, Vincent B. Masto, Kathy H. Li, Raul Andino, Judith Frydman1:1,000), rabbit anti-RPS2 (Bethyl Laboratories A303–794, 1:1,000), rabbit anti-RPS3 (Cell Signaling 9538, 1:1,000), rabbit anti-Atf4 (Cell Signaling 11815, 1:1,000), goat anti-Poliovirus VP1 (Thermo Fisher

(14) Proteomic Profiling of Chemotherapy Responses in FOLFOX-Resistant Colorectal Cancer CellsInternational journal of molecular sciencesJune 8, 2023Tam SY, Islam Khan MZ, Chen JY, Yip JH, Yan HY, Tam TY, Law HK.USA), phosphor-RAC1 (Ser71) (#PA5-104640, Invitrogen), RPL26 (D8F6) (#5400, CST) and RPS3 (D50G7) (#9538, CST) at 4 °C and 1:1000 concentration in TBST with 5% BSA. The membrane was then incubated by the secondary

(15) The ufmylation modification of ribosomal protein L10 in the development of pancreatic adenocarcinomaCell death & diseaseJune 7, 2023Wang K, Chen S, Wu Y, Wang Y, Lu Y, Sun Y, Chen Y.antibody (Santa Cruz, #H2409), anti-UFM1 antibody (Abcam, #ab109305), anti-RPS3 antibody (CST technology, #9538) or anti-flag antibody (CST technology, #8146) for 16 h at 4 °C. Then, 30 μl Agarose (Millipore, Massachusetts,

(16) BTG1 mutation yields supercompetitive B cells primed for malignant transformationScience (New York, N.Y.)January 20, 2023Mlynarczyk C, Teater M, Pae J, Chin CR, Wang L, Arulraj T, Barisic D, Papin A, Hoehn KB, Kots E, et al.anti-MYC (rabbit, Cell Signaling Technology 5605, 1:1000), or anti-RPS3 (rabbit, Cell Signaling Technology 9538, 1:3000 in 5% BSA). Membranes were then incubated with HRP-conjugated anti-mouse or anti-rabbit secondary

(17) Spautin-1 inhibits mitochondrial complex I and leads to suppression of the unfolded protein response and cell survival during glucose starvationScientific ReportsDecember 1, 2022Kazuhiro Kunimasa, Chika Ikeda-Ishikawa, Yuri Tani, Satomi Tsukahara, Junko Sakurai, Yuka Okamoto, Masaru Koido, Shingo Dan, Akihiro Tomida(Thr389) (Cat#: 9234), anti-phospho-eIF2α (Ser51) (Cat#: 3398), anti-RPL7 (Cat#: 2403), anti-RPS3 (Cat#: 9538) (all from Cell Signaling Technology, Danvers, MA, USA), anti-eIF2α (Cat#: ab5369; Abcam, Cambridge,

(18) Redox-sensitive E2 Rad6 controls cellular response to oxidative stress via K63-linked ubiquitination of ribosomesCell reportsMay 24, 2022Simões V, Cizubu BK, Harley L, Zhou Y, Pajak J, Snyder NA, Bouvette J, Borgnia MJ, Arya G, Bartesaghi A, et al.PA1-981), anti-Rad6 (1:8,000; Abcam, cat. no. ab31917), anti-Rps3/uS3 (1:6,000; Cell Signaling, cat. no. 9538S), anti-Rpl11/uL5 (1:6,000; Cell Signaling, cat. no. 18163S), anti-HA (1:10,000; ThermoFisher, cat. no.

(19) Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codonsNucleic acids researchJuly 21, 2021Ichihara K, Matsumoto A, Nishida H, Kito Y, Shimizu H, Shichino Y, Iwasaki S, Imami K, Ishihama Y, Nakayama KI.(Thermo Fisher Scientific, PA5-23278), eIF3D (Bethyl Laboratories, A301-758A), RPS3 (Cell Signaling Technology, 9538), RPL5 (Cell Signaling Technology, 14568), ATF4 (Proteintech, 10835-1-AP), V5 tag (Thermo Fisher Scientific,

(20) Dual Inhibition of DKC1 and MEK1/2 Synergistically Restrains the Growth of Colorectal Cancer CellsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2021Kan G, Wang Z, Sheng C, Chen G, Yao C, Mao Y, Chen S.(Affinity Biosciences, Cincinnati, OH, USA, #DF3708), RPS3 (Cell Signaling Technology, Boston, USA, #9538), Myc (RM1003, Ray Antibody, Beijing, China), Flag (Sigma, St. Louis, USA, #F1804), and His (Proteintech,

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9/26/2019Highly Sensitive and Specific Antibody against RPS3Dr. H. AnI recommend this antibody to others.

Ribosomal Protein S3 (D50G7) XP ® Rabbit mAb

Ribosomal Protein S3 (D50G7) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Ribosomal Protein S3 (D50G7) XP ® Rabbit mAb from Cell Signaling Technology

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