For immunofluorescence analysis,THP-1 cells were fixed and permeabilized for detection of endogenous TREM2 using Anti-TREM2 Recombinant Rabbit Monoclonal Antibody (Product # 702886, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representative control cells that were stained for detection and localization of TREM2 protein (green) with reduced membrane signal in undifferentiated cells compared to panel e-h) clearly showing enhanced membrane localisation in cells differentiated to dendritic cell phenotype demonstarting specificity. The images were captured at 60X magnification.
For immunofluorescence analysis,THP-1 cells were fixed and permeabilized for detection of endogenous TREM2 using Anti-TREM2 Recombinant Rabbit Monoclonal Antibody (Product # 702886, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representative control cells that were stained for detection and localization of TREM2 protein (green) with reduced membrane signal in undifferentiated cells compared to panel e-h) clearly showing enhanced membrane localisation in cells differentiated to dendritic cell phenotype demonstarting specificity. The images were captured at 60X magnification.
Supplier Page from Thermo Fisher Scientific for TREM2 Antibody