Fig 2: Mast cell-derived exosomal miR-181a-5p regulated trophoblast cell migration and invasion. (A, B) The effect of up- or down-regulation of miR-181a-5p on miR-181a-5p level in exosomes or co-culture systems was detected by qRT-PCR. (C) The effect of mast cell-derived miR-181a-5p on the viability of trophoblast cells was detected by MTT. (D, E) The effect of miR-181a-5p up- or down-regulation in exosomes or co-culture systems on trophoblast cell migration was determined using wound healing assay. (F, G) The cell invasion in each group was detected by Transwell. (H) Western blot was applied to measure the protein expressions of YY1, N-cadherin, E-cadherin, Vimentin, and MMP-9, with GAPDH as the control. ## p < 0.01, ### p < 0.001 versus MC; ^ p < 0.05, ^^^ p < 0.001 versus IC; +++ p < 0.001 versus control (HTR-8/SVneo cells)

Fig 3: YY1 overexpression reversed the regulation of mast cell-derived exosomal miR-181a-5p on trophoblast cells. (A) After co-culture of exosomes containing miR-181a-5p mimic or mimic control with YY1-overexpressing HTR-8/SVneo cells, cell viability was detected by MTT assay. (B, C) Cell migration rates were measured in wound healing assays 48 h after cell treatment. (D, E) Transwell assay was employed to determine the effects of YY1 overexpression and exosomal miR-181a-5p on trophoblast cell invasion. (F) The protein expressions of N-cadherin, E-cadherin, Vimentin and MMP-9 were detected by Western blot. ?? p < 0.01, ??? p < 0.001 versus MC-exo + NC; †† p < 0.01, ††† p < 0.001 versus MC-exo + YY1; OO p < 0.01, OOO p < 0.001 versus M-exo + NC

Fig 4: YY1 targeted by miR‐181a‐5p. (A) TargetScan (http://www.targetscan.org/vert_72/) was used to predict the targeting relationship of miR‐181a‐5p to YY1. (B) Dual‐luciferase reporter assay was used to verify the binding of miR‐181a‐5p to YY1. (C) Transfection of YY1 overexpression plasmid increased YY1 level in HTR‐8/SVneo cells as detected by qRT‐PCR. δδδ p < 0.001 versus YY1‐WT + MC; ∆∆∆ p < 0.001 versus NC (negative control)