Fig 1: Increased macrophage CD68 expression indicative of enhanced phagocytic activity, in vitro. A MP images representing unaltered CD206 expression, M2 macrophages, in 28w and 57w GlaKO mice. Scale bar 50 µm. Male: 28 weeks wt mice n = 4, GlaKO mice n = 4, 57 weeks wt mice n = 4, GlaKO mice n = 6. B Aged GlaKO mice showed a significant upregulation of the endo/lysosomal marker CD68 in DRG. Scale bar 50 µm. Male: 28 weeks wt mice n = 4, GlaKO mice n = 4, 57 weeks wt mice n = 4, GlaKO mice n = 7. C Lyso-Gb3 or (D) Gb3 activated phagocytosis of BV-2 (1% DMSO was used as vehicle). Wells per condition n = 6. Statistical significance was assessed using Kruskal–Wallis test followed by pairwise comparisons using Wilcoxon rank sum exact test and Bonferroni correction. Values are given as mean ± SEM. * p < 0.05, ** p < 0.01, if not otherwise stated