Fig 1: (A) Relative protein expression level of the proinflammatory‐related gene Il‐1β in the peripheral blood in the three groups (WT, wild‐type mice intravenous (IV) saline administration; 5FAD, 5×FAD mice IV saline administration; 5FAD+DNT, 5×FAD+DNT, 5×FAD mice IV DNT administration) were detected by ELISA. n = 3 mice per group; means ± SEM, ns, nonsignificant; *p < 0.05; **p < 0.01; unpaired Student's t‐test. (B, C) Western Blotting of anti‐inflammatory and proinflammatory cytokines in the whole brain tissue after IV DNT treatment. n = 3 mice per group; mean ± SEM, ns, nonsignificant, *p < 0.05; unpaired Student's t‐test. (D) Principal component analysis (PCA) of the saline‐treated wild‐type mice (WT1,2), and the AD mice brain tissue bulk transcriptomes with (DNT1,2) or without DNT treatment but saline treatment (AD1,2). (E) Heatmap of changed gene expression in brain tissues of the three groups of mice by RNA‐seq. Each column represents expression levels from one individual mouse, n = 2 mice per group. Color code presents a linear scale.