Alexa Fluor® 488 anti-mouse CD3ε 100321 from BioLegend

Product Specs
ItemAlexa Fluor® 488 anti-mouse CD3ε
CompanyBioLegend
Price $232.00Supplier Page View Company Product Page
Catalog Number100321
NCBI Gene AliasesCD3, CD3epsilon, T3e
Quantity100UG
ReactivityMouse
IsotypeArmenian Hamster IgG
ApplicationsFC
Conjugate/TagAlexa Fluor® 488
HostArmenian Hamster
Clone145-2C11
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, epsilon polypeptide
Concentration0.5000mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenH-2K^b-specific mouse cytotoxic T lymphocyte clone BM10-37
ClonalityMonoclonal
TargetCD3epsilon

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Longitudinal microneedle sampling resolves tissue-restricted immune-microbiome dynamics in skinmedRxivMay 5, 2026Ashok Kumar Dhinakaran, Anita Yvonne Voigt, Arthur Szacik, Soo Yeon Kang, Sebastian Giarratana, Julia Oh, Sasan Jaliliantibodies were used for surface staining: CD45 (BUV395, Cat. 564279, BD Biosciences), CD3ε (AF488, Cat. 100321, BioLegend), CD11b (BV785, Cat. 101243, BioLegend), NK1.1 (BV421, Cat. 108732, BioLegend), F4/80 (PE,

(2) Directed evolution-based discovery of ligands for in vivo restimulation of chimeric antigen receptor T cellsNature Biomedical EngineeringAugust 25, 2025Tomasz M. Grzywa, Alexandra Neeser, Ranjani Ramasubramanian, Anna Romanov, Ryan Tannir, Naveen K. Mehta, Benjamin Cossette, Duncan M. Morgan, Beatriz Goncalves, Ina Sukaj, et al.treated with Fc blocker (Innovex) and stained with antibody solution (1:100 anti-CD3e AF488 (BioLegend 100321), 1:100 anti-B220 AF594 (BioLegend 103254) and 1:75 anti-CD11c AF647 (BD Biosciences 565587)) diluted

(3) SMAD4 and KRAS Status Shape Cancer Cell-Stromal Crosstalk and Therapeutic Response in Pancreatic CancerCancer ResApril 15, 2025Eloise G. Lloyd, Muntadher Jihad, Judhell S. Manansala, Wenlong Li, Priscilla S.W. Cheng, Gianluca Mucciolo, Marta Zaccaria, Sara Pinto Teles, Joaquín Araos Henríquez, Sneha Harish, et al.(108749; Biolegend; RRID:AB_2564304), TCRβ-Alexa488 (109215; Biolegend; RRID:AB_493344); CD3ε-Alexa488 (100321; Biolegend; RRID:AB_389300), CD4-APC (100516; Biolegend; RRID:AB_312719) and CD8a-APC/Cy7 (100713; Biologend;

(4) SMAD4 and KRAS Status Shape Cancer Cell-Stromal Crosstalk and Therapeutic Response in Pancreatic CancerCancer ResearchJanuary 22, 2025Eloise G. Lloyd, Muntadher Jihad, Judhell S. Manansala, Wenlong Li, Priscilla S. W. Cheng, Gianluca Mucciolo, Marta Zaccaria, Sara Pinto Pinto Teles, Joaquín Araos Araos Henríquez, Sneha Harish, et al.(108749; Biolegend; RRID:AB_2564304), TCRβ-Alexa488 (109215; Biolegend; RRID:AB_493344); CD3ε-Alexa488 (100321; Biolegend; RRID:AB_389300), CD4-APC (100516; Biolegend; RRID:AB_312719) and CD8a-APC/Cy7 (100713; Biologend;

(5) Evaluation of STK17B as a cancer immunotherapy target utilizing highly potent and selective small molecule inhibitorsFrontiers in ImmunologyOctober 21, 2024Felix Scheuplein, Florian Renner, John E. Campbell, Robert Campbell, Chris De Savi, Jan Eckmann, Holger Fischer, Jie Ge, Luke Green, Peter Jakob, et al.USA) Antibodies from BioLegend were used for flow cytometry for the detection of CD3 (Clone 145-2C11 Cat# 100321) 1:100 dilution, CD8 (Clone 53-6.7 Cat # 100732) 1:100 dilution, IL-2 receptor alpha chain (CD25) (Clone

(6) Enrichment of novel CD3+F4/80+ cells in brown adipose tissue following adrenergic stimulationFrontiers in ImmunologyAugust 27, 2024Hee-Don Chae, Jelena Leviantibodies. The following fluorochrome-conjugated antibodies were used: Alexa Fluor® 488 anti-mouse CD3ϵ (#100321), Brilliant Violet 510™ anti-mouse CD4 (#100449), PerCP/Cyanine5.5 anti-mouse CD8a (#100734), PerCP/Cyanine5.5

(7) Primary nasal influenza infection rewires tissue-scale memory response dynamicsImmunityAugust 1, 2024Samuel W. Kazer, Colette Matysiak Match, Erica M. Langan, Marie-Angèle Messou, Thomas J. LaSalle, Elise O’Leary, Jessica Marbourg, Katherine Naughton, Ulrich H. von Andrian, Jose Ordovas-MontanesAB_2814069" "CCR2, APC",R&D Systems,Cat#FAB5538A-100; Clone 475301 "CD3ε, Alexa Fluor® 488",Biolegend,Cat#100321; Clone 145–2C11; RRID: AB_389300 "CD11b, Alexa Fluor® 594",Biolegend,Cat#101254; Clone M1/70; RRID:

(8) Generating an organ-deficient animal model using a multi-targeted CRISPR-Cas9 systemScientific reportsMay 9, 2024Lim JJ, Murata Y, Yuri S, Kitamuro K, Kawai T, Isotani A.100516), PE anti-mouse CD3ε (1:50, Biolegend 100308), Alexa Fluor 488 anti-mouse CD3ε (1:50, Biolegend 100321), APC anti-mouse CD4 (1:50, Biolegend 100516), PerCP anti-mouse CD8 (1:50, Biolegend 100732), APC anti-rat

(9) Leukemic mutation FLT3-ITD is retained in dendritic cells and disrupts their homeostasis leading to expanded Th17 frequencyFrontiers in immunologyJanuary 1, 2024Flynn PA, Long MD, Kosaka Y, Long N, Mulkey JS, Coy JL, Agarwal A, Lind EF.Bio CD45.2,APC-Cy7,104,109824,BioLegend CD11c,BV421,N418,117330,BioLegend CD8a,PacificBlue,53-6.7,100725,BioLegend CD44,BV605,IM7,103407,BioLegend NK1.1,BV650,PK136,108736,BioLegend CD19,BV711,605,115555,BioLegend CD3e,Alexa Fluor488,145-2C11,100321,BioLegend I/A-I/E,PerCP,M5/114.15.2,107624,BioLegend CD4,PerCP-Cy5.5,RM4-4,116012,BioLegend Zombie

(10) Targeting PGLYRP1 promotes antitumor immunity while inhibiting autoimmune neuroinflammationNature immunologyNovember 1, 2023Schnell A, Huang L, Regan BML, Singh V, Vonficht D, Bollhagen A, Wang M, Hou Y, Bod L, Sobel RA, et al.following antibodies were purchased from Biolegend (clone, catalog number, dilution): CD3 (145–2C11, 100321, 1:500), CD4 (RM4–5, 100512, 1:500), CD8 (53–6.7, 100708, 1:500), CD11b (M1/70, 101212, 1:500), CD11c

(11) Network analysis of large-scale ImmGen and Tabula Muris datasets highlights metabolic diversity of tissue mononuclear phagocytesCell reportsFebruary 28, 2023Gainullina A, Mogilenko DA, Huang LH, Todorov H, Narang V, Kim KW, Yng LS, Kent A, Jia B, Seddu K, et al.RRID:AB_493568 CD11c PE clone N418,Biolegend,Cat#117308; RRID:AB_313777 CD3e AF488 clone 145-2C11,Biolegend,Cat#100321; RRID:AB_389300 CD19 FITC clone 1D3/CD19,Biolegend,Cat#152404; RRID:AB_2629813 NK1.1 AF488 clone PK136,Biolegend,Cat#108718;

(12) Do more with Less: Improving High Parameter Cytometry Through Overnight StainingCurr ProtocNovember 1, 2022Whyte CE, Tumes DJ, Liston A, Burton OT.Violet 421,GK1.5,1:1000,3,BioLegend,100443 Anti‐mouse CD3 Alexa Fluor 488,145‐2C11,1:1000,3,BioLegend,100321 Anti‐mouse CCR7 APC‐Fire 810,4B12,"1:50, 1:200",4,BioLegend,120135 Anti‐mouse CD3 Spark Blue 550,17A2,"1:500,

(13) Intratumoral Treatment with 5-Androstene-3β, 17α-Diol Reduces Tumor Size and Lung Metastasis in a Triple-Negative Experimental Model of Breast CancerInternational journal of molecular sciencesOctober 8, 2022Ruiz Manzano RA, Nava-Castro KE, Palacios-Arreola MI, Hernández-Cervantes R, Del Río-Araiza VH, Segovia-Mendoza M, Pérez-Torres A, Girón-Pérez MI, Morales-Montor J.were stained with the following panels. For T lymphocyte: AlexaFluor 488-conjugated anti-CD3ε (Cat# 100321, Clone 145-2C11, RRID:AB_389301) 1:100, PE-conjugated anti-CD4 (Cat# 100407, Clone GK1.5, RRID:AB_2075573)

(14) Selective activation and expansion of regulatory T cells using lipid encapsulated mRNA encoding a long-acting IL-2 muteinNature communicationsJuly 5, 2022de Picciotto S, DeVita N, Hsiao CJ, Honan C, Tse SW, Nguyen M, Ferrari JD, Zheng W, Wipke BT, Huang E.eFluor780 (ThermoFischer L34961) at 1:100 dilution, and surface antibodies CD3e AF488 (1:400, Biolegend 100321), CD4 BV510 (1:1600, Biolegend 100449), CD8a BV570 (1:400, Biolegend 100740), NK1.1 AF700 (1:800, Biolegend

(15) The impact of CD160 deficiency on alloreactive CD8 T cell responses and allograft rejectionTranslational research : the journal of laboratory and clinical medicineJanuary 1, 2022Del Rio ML, Nguyen TH, Tesson L, Heslan JM, Gutierrez-Adan A, Fernandez-Gonzalez R, Gutierrez-Arroyo J, Buhler L, Pérez-Simón JA, Anegon I, et al.

(16) Itaconate confers tolerance to late NLRP3 inflammasome activationCell reportsMarch 9, 2021Bambouskova M, Potuckova L, Paulenda T, Kerndl M, Mogilenko DA, Lizotte K, Swain A, Hayes S, Sheldon RD, Kim H, et al.monoclonal Ab",Biolegend,Cat# 653904; RRID:AB_2564508 "CD3, AF488, hamster monoclonal Ab",Biolegend,Cat# 100321; RRID:AB_389300 "CD4, BV711, rat monoclonal Ab",Biolegend,Cat# 100549; RRID:AB_11219396 "CD8, BV421,

(17) Comprehensive Profiling of an Aging Immune System Reveals Clonal GZMK⁺ CD8⁺ T Cells as Conserved Hallmark of InflammagingImmunityJanuary 12, 2021Mogilenko DA, Shpynov O, Andhey PS, Arthur L, Swain A, Esaulova E, Brioschi S, Shchukina I, Kerndl M, Bambouskova M, et al.

(18) Humanized Mouse Model as a Novel Approach in the Assessment of Human Allogeneic Responses in Organ TransplantationFrontiers in immunologyJanuary 1, 2021Ajith A, Mulloy LL, Musa MA, Bravo-Egana V, Horuzsko DD, Gani I, Horuzsko A.1:300); Anti-human/mouse antibody Granzyme B (515403, clone GB11, 1:300); Anti-mouse antibodies: CD3 (100321, clone 145-2C11, 1:300), CD4 (100407, clone GK1.5, 1:200), CD8 (100713, clone 53-6.7, 1:300), and CD25

(19) Digenic mutations in ALDH2 and ADH5 impair formaldehyde clearance and cause a multisystem disorder, AMeD syndromeScience advancesDecember 1, 2020Oka Y, Hamada M, Nakazawa Y, Muramatsu H, Okuno Y, Higasa K, Shimada M, Takeshima H, Hanada K, Hirano T, et al.421, no. 135313, BioLegend), CD34 (APC/Fire 750, no. 135014, BioLegend), CD3ε (Alexa Fluor 488, no. 100321, BioLegend), CD19 (APC, no. 152410, BioLegend), CD4 (PE, no. 130310, BioLegend), and CD8a (APC, no.

(20) SOX2 promotes resistance of melanoma with PD-L1 high expression to T-cell-mediated cytotoxicity that can be reversed by SAHAJournal for immunotherapy of cancerNovember 1, 2020Wu R, Wang C, Li Z, Xiao J, Li C, Wang X, Kong P, Cao J, Huang F, Li Z, et al.Cell Signaling Technology (Danvers, MA, USA). Flow cytometry antibodies: Alexa Fluor 488 antimouse CD3ε (No. 100321), Alexa Fluor 647 antihuman/mouse GranzymeB (No. 515406) and PerCP/Cyanine5.5 antimouse IFNγ (No. 505821)

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1/7/2019Successful Detection of Tumour Infiltrating T cells Jamie WallisThis antibody was used to quantify T cell infiltration into tumours. Staining was clear but required a lot of antibody.

International journal of molecular sciences

Frontiers in oncology

International journal of molecular sciences

Frontiers in oncology

International journal of molecular sciences

Alexa Fluor® 488 anti-mouse CD3ε from BioLegend

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Alexa Fluor® 488 anti-mouse CD3ε from BioLegend

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