Fig 1: Flow cytometry analysis to characterize iPSC-RPCs and iPSC-RGCs. (A,B) FACS analysis of iP SC-RPCs at day 15 showed that over 95.5% and 82% of the cells expressed ki67 and Chx10, respectively. About 82% of the cells expressed both the RPC markers. (C–F) FACs analysis at day 35 showed that 87%, 93%, 85.5%, and 22.5% of the iPSC-RGCs were positive for BRN3, SNCG, CD90, and RBPMS, respectively. (G) A subset of the iPSC-RGC population (insert from (D)) expressed all the four RGC markers; namely 87% of the cells were positive for BRN3 and SNCG, whereas 81% was positive for CD90 and 19% was positive for RBPMS.
Fig 2: Immuno-staining of the transplanted hiPSC-RGCs in mouse retina with RGC-specific markers(A–H) Flattened whole-mount images of transplanted hiPSC-RGCs (n = 8) were co-stained with RGC-specific markers (A) BRN3, (B) RBPMS, (C) TUJ1, (D) MAP2, (E) VGLUT2, and (F) PSD95. Donor hiPSC-RGCs were also co-stained with (G) human nuclear antigen (HNA) and (H) Ku80 to confirm the donor origin of SNCG-eGFP + cells. * Since the human nuclear antigen antibody was raised in mice, some cross-reactivity with the murine retina was observed. Z-1: Zoom-1 (scale bar is 10 µm), Z-2: Zoom-2 (scale bar is 10 µm), and Z-4: Zoom-4 (scale bar is 5 µm).
Supplier Page from Novus Biologicals, a Bio-Techne Brand for RBPMS Antibody (OTI2G10) [Alexa Fluor® 647]
Available conjugates: Available conjugates: Alexa Fluor 647Sizes Available: 0.1 ml