Western blot analysis of Phospho-Rb (S807) on different lysates with Rabbit anti-Phospho-Rb (S807) antibody (ET1602-36) at 1/2,000 dilution. Lane 1: K-562 cell lysate (no heat) Lane 2: K-562 cell lysate Lane 3: K-562 cell lysate (no heat), treated with λpp for 1 hour Lane 4: K-562 cell lysate, treated with λpp for 1 hour Notice: no heat means the lysate is not boiled. Lysates/proteins at 15 µg/Lane. Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-36) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.