Western blot analysis of eIF4EBP1 on different lysates with Rabbit anti-eIF4EBP1 antibody (ET1701-83) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: L6 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse skeletal muscle tissue lysate Lane 7: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 13 kDa Observed band size: 17/18 kDa Exposure time: 26 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-83) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.